Freeze-drying protection method and freeze-drying protection solution for biomolecule or cell enrichment screen
A technology of freeze-drying protection solution and biomolecules, which is applied in the field of freeze-drying protection methods and freeze-drying protection solutions of biomolecules or cell enrichment screens, and can solve problems such as damage, high concentration of plasticizer sucrose, and formation. Achieve excellent resolubility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Example 1: Evaluation of excipient recalloration
[0048] The excipients of the lyophilized protective liquid were prepared according to the ratio of the following Table 1, and the solvent was PBS buffer.
[0049] The formulation of the test phase reagent is as follows:
[0050] 1) Mother liquor 1: 20% sucrose PBS solution: 20 g Sucrose is dissolved in 100 mL of PBS buffer (a pH of 7.2, concentration of 1 mol / L);
[0051] 2) Mother liquor 2:20% mannitol PBS solution: 20 g Sucrose is dissolved in 100 mL of PBS buffer (a pH of 7.2, concentration is 1 mol / L);
[0052] 3) Mother liquor 3: 20% sorbitol PBS solution: 20 g Sucrose is dissolved in 100 mL of PBS buffer (a pH of 7.2, concentration is 1 mol / L);
[0053] 4. Mother liquor 4: thickened preservative: 2G PVP K30, 10 mg thiosa mercury is dissolved in 100 mL of PBS buffer (a pH of 7.2, concentration is 1 mol / L);
[0054] The above four kinds of mother liquors were filtered through a 5.22 μmol of filtration and steril...
Embodiment 2
[0063] Example 2: Evaluation of the protective effect of lyophilized protective solution on polymer materials on enrichment screen
[0064] Test Scheme: In the enriched screen processing engineering, after the incubation of polymer materials (mainly hyaluronic acid (HA)), the antibody is no longer continued, and the frozen of the formulation based on Embodiment 1 is directly performed. Lyophilization treatment of dry protective liquid. After the lying is completed, accelerated aging experiment, accelerated aging conditions at a constant temperature of 45 ° C, and the experiment selection 3 nodes, accelerated aging treatment for 7 days, 14 days, 21 days, and 5 techniques for each group. After the accelerated aging time is completed, the dry matter residual removal of the lyophilized protective liquid is removed using PBS, and then the normal antibody package is carried out. In view of the presence of antibody adsorption characteristics in the form of the hole plate used, the absorb...
Embodiment 3
[0068] Example 3: Evaluation of the protective effect of lyophilized protective solution on the effect of the coated screen to be protected by antibody
[0069] In order to more intuitively evaluate the protective effect of the lyophilized protective liquid to the mesh chip binding antibody, EPCAM antibody after lyophilizing liquid lyophilized liquid lyophilized liquid liquid liquid obtained by ELISA is evaluated. Based on the ability of the secondary antibody labeled with HRP, the developing reaction of the HRP catalytic substrate TMB is reacted in the form of the mesh chip in the form of the mesh chip in the lyophilized antibody activity. Experimental data is shown in Table 3.
[0070] table 3
[0071]
[0072] Conclusion: The lyophilized protective liquid of the present embodiment has no effect on the effect of the EPCAM antibody on the mesh chip, and the lyophilized protective liquid can protect the activity of the EPCAM antibody bonded to the mesh chip.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More