Freeze-drying protective agent and application thereof

Abstract

The invention discloses a freeze-drying protective agent and application thereof. The freeze-drying protective agent comprises the following components in parts by weight: 5-20 parts of stachyose; 10 to 30 parts of maltodextrin; 0.01 to 0.2 part of gelatin; 0.1 to 5 parts of a surfactant; and 100 parts of water. The freeze-drying protective agent is applied to preparation of freeze-drying microspheres of a real-time fluorescent quantitative PCR reaction solution. The freeze-drying microspheres prepared by adopting the freeze-drying protective agent disclosed by the invention have the characteristics of regular form, stable structure, smooth and round surface, low hygroscopicity and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the field of PCR (polymerase chain reaction) reagent preparation, in particular to a protective agent for preparing real-time fluorescent quantitative PCR reaction liquid freeze-dried microspheres. Background technique [0002] In recent years, PCR reagents have been widely used in food testing, pathogen testing, and cancer gene diagnosis. However, PCR reagents have strict requirements on storage and transportation conditions. Generally, they need to be stored at -20°C and there are restrictions on the number of freeze-thaw solutions. Dry ice transportation is generally required. If these conditions are not met, the performance of the PCR reagent will be greatly affected, and even the reagent will fail. [0003] In order to solve the above problems, freeze-dried reagents have appeared, and the technical route includes two methods: direct freeze-drying in PCR reaction tubes and liqui...

AI Technical Summary

Problems solved by technology

In terms of function, because liquid nitrogen granulation and freeze-dried microspheres need to be sorted and packaged, if the structure of the spheres is irregular, the mechanical strength of the spheres will be unstable, which will affect the subsequent production steps. Affect the stability of the reagent

However, it is difficult to prepare microspheres with a complete structure. The reason is that when liquid nitrogen granules or mold granules are formed into balls, the requirements for protective agents are very high, and the function of qPCR (real-time fluorescent quantitative PCR) reagents cannot be affected. , it is also necessary to ensure a certain mechanical strength, and it is necessary to conduct in-depth research on the protective agent

Second, the problem of moisture absorption of microspheres after freeze-drying

But even so, if exposed to the air for a long time, it will cause deformation and collapse of the ball due to moisture absorption, causing production quality problems

Although directly freeze-dried into PCR tubes, there is also the problem of moisture absorption after freeze-drying, but freeze-dried microspheres are more likely to deform and collapse the spherical structure due to moisture absorption

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