Preparation method and use method of injectable bionic antioxidant hydrogel

A hydrogel and antioxidant technology, applied in medical science, bandages, etc., can solve the problems of limited normal activities, re-infection, and inability to keep the wound moist, so as to solve the problem of insufficient contact time and reduce the pain of patients.

Pending Publication Date: 2022-04-12
GUANGXI XINYE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the coverage of the wound dressing is not easy to fix. When using it, it is necessary to pay attention to waterproofing, and the wound dressing needs to be changed, etc., or the fixation of the wound restricts normal activities and cannot keep the wound moist. There are many

Method used

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  • Preparation method and use method of injectable bionic antioxidant hydrogel

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Example Embodiment

[0021] Example 1

[0022] Step (1) Combined with the complex of the composite protein: Take 0.4 g of mussel protein, 0.2 g of silk fibroin, 0.07 g of hyaluronate, 0.15 g of acetyl cysteine, mixed into 100 ml of water and mix evenly obtained a uniform solution Then, the homogeneous solution was obtained using a rotary evaporation method to obtain a concentrated dialysis bag, and the dialysis bag was placed in sterilized distilled water, and the dialysis was completed for freeze drying to obtain a composite protein powder;

[0023] Step (2) Preparation of VE, VC object clamp: The composite protein powder of step (1) is 0.005 g to dissolve with 100 ml of sterile water under sterile conditions, stirring to obtain a composite solution; then the 10 ml volume concentration is 0.1% of VE-ethanol solution drift into the composite protein solution, a shaker of 40 ° C is placed in a shaker at 40 ° C, distilled down 40 ° C to 50 mL to obtain 50 mL of a viscous aqueous solution, add 0.1 gVc, 0...

Example Embodiment

[0031] Example 2

[0032] A method of preparing an injectable bionic antioxidant hydrogel, which includes the steps of:

[0033] Step (1) Combined with the complex of the composite protein: Take 0.5 g of mussel protein, 0.25 g of silkase protein, 0.08 g of sodium hyaluronate, 0.16 g of acetyl cystine, mixed into 100 ml of water and mix evenly obtained a uniform solution Then, the homogeneous solution was obtained using a rotary evaporation method to obtain a concentrated dialysis bag, and the dialysis bag was placed in sterilized distilled water, and the dialysis was completed for freeze drying to obtain a composite protein powder;

[0034] Step (2) Preparation of VE, VC object clamp: Composite protein powder of step (1) 0.01 g of 100 ml of sterile water dissolved under sterile conditions, stirring to obtain a composite protein solution; then subjected to a composite protein solution; then a 10 ml volume concentration is 0.1% of the VE-ethanol solution drift into the composite pro...

Example Embodiment

[0042] Example 3

[0043] Step (1) Combined with complex protein: Take 0.6 g of mussel protein, 0.3 g of silk fibroin, 0.09 g of sodium hyaluronate, 0.17 g of acetyl cystine, mixed into 100 ml of water and mix the uniform solution Then, the homogeneous solution was obtained using a rotary evaporation method to obtain a concentrated dialysis bag, and the dialysis bag was placed in sterilized distilled water, and the dialysis was completed for freeze drying to obtain a composite protein powder;

[0044] Step (2) Preparation of VE, VC Object Packing: The composite protein powder of step (1) is 0.015 g to dissolve with 100 ml of sterile water under sterile conditions, stirring to obtain a composite solution; then the 10 ml volume concentration is 0.1% of the VE-ethanol solution drift into the composite protein solution, and the shaker of 50 ° C was placed at 50 ° C to distillation, and the solution was evaporated under reduced pressure to obtain 50 mL of the viscous aqueous solution, ...

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Abstract

The invention relates to the technical field of hydrogel, in particular to a preparation method and a use method of injectable bionic anti-oxidation hydrogel, and the injectable bionic anti-oxidation hydrogel is constructed through crosslinking synthesis of compound protein, preparation of VE and VC guest inclusion complexes and preparation of the anti-oxidation hydrogel. The wound dressing can simulate a cell antioxidant defense system to construct the wound dressing, eliminate the overexpression of reactive oxygen species (ROS) in wound tissues, reduce the damage of the overexpression ROS to cells, tissues and organs, not only can eliminate extracellular reactive oxygen of skin tissues, but also can effectively eliminate reactive oxygen in cells of the skin tissues; the tissue adhesion and the self-healing capability of the wound dressing help the wound dressing to resist mechanical deformation caused by limb movement, and the problems that the dressing is insufficient in contact time and low in durability are effectively solved.

Description

technical field [0001] The invention relates to the technical field of hydrogels, in particular to a method for preparing and using an injectable biomimetic anti-oxidation hydrogel. Background technique [0002] Oxidative stress injury is one of the important mechanisms of multiple organ dysfunction in early burn patients. In the early stage of severe burns, a large amount of body fluid is lost. A large amount of reactive oxygen species (ROS) produced in the early stage of burn can damage the lipid, protein and nucleic acid components of tissues and cells, and cooperate with inflammatory factors and vasoactive substances to cause changes in cell structure and function, resulting in severe oxidation. damage. [0003] At present, traditional wound treatment and repair methods are mainly covered by wound dressings. For example, patent CN111265710A discloses a preparation method of a new type of bionic skin wound dressing based on MAP / PRF composite. Biomimetic synthesis of MA...

Claims

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Application Information

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IPC IPC(8): A61L26/00
Inventor 苏雪梁文棋梁奕福梁超余曾金萍方祖丽李艺华辜衍丽余勇华
Owner GUANGXI XINYE BIOLOGICAL TECH
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