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Lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of lactobacillus jensenii

A Lactobacillus jansnii, bacterial technology, applied in the field of Lactobacillus jansii, can solve the problem of less Lactobacillus and achieve the effect of reducing the possibility

Active Publication Date: 2022-04-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few effective lactobacilli that can regulate immunity, and further excavation and development are needed.

Method used

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  • Lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of lactobacillus jensenii
  • Lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of lactobacillus jensenii
  • Lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of lactobacillus jensenii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Lactobacterium inhibited vaginal Gardney biofilm formation

[0044] Centrifuge at 4 ° C for 10 min, collect the concentration of corresponding cultures 10 8 CFU / ml of Lactobacillus supernatant. Culture of BHIS medium in vaginal Gardini 24h to 10 8 CFU / ml, transfer 100 μl of vaginal Gednini suspension to 96-well plates, add 100 μl of Lactobacillus supernatant. MRS is used as a blank control. The plate was incubated at 37 ° C for 24 h. After 24 hours of biofilm formation, the floating cell was taken 3 times with PBS, and 100 μl of methanol (National Medicine Group Chemical Reagent Co., Ltd.) was added to 30 minutes per well. After abandoning methanol, the biofilm was crystallized from 0.1% crystalline purple to 5 minutes. The crystalline purple was discarded and washed 3 times with PBS, and 200 μl of 33% ice acetic acid (National Medicine Group Chemical Reagent Co., Ltd.) per well was added. The absorbance of each hole at 570 nm was measured by the enzyme gauge...

Embodiment 2

[0048] Example 2: Adhesion of Jean Lactobacillus

[0049] The adhesion capacity of Lactobacillus has been reported in previous studies to suppress pathogens in vaginal environments, and regulate the immune defense response of host localities, and thus evaluate the adhesion of Lactifer.

[0050] Test of in vitro adhesion:

[0051] The growth of 80% of HeLa cells was digested, and the sterile cover slides were placed in a 6-well culture plate, and 2 ml / well cells were completely cultured (1 × 10). 5 1 / ml), 5% CO is placed under 37 ° C 2 Culture in the incubator, after the cell is attached, discard the medium, add 2 ml / well lactoform suspension (1 × 10 8 CFU / mL) and then incubated for 2 h. After the end, was washed with phosphate buffer to remove unneained Lactobacillus, and the methanol was fixed for 20 min to perform Gram dye, microscope observation. 20 fields were randomly selected for evaluation, and the number of bacteria adhered to 100 cells was raided, and the number o...

Embodiment 3

[0053] Example 3: Application of Jean Lactobacillus CCFM1213 in alleviating bacterial vaginitis

[0054] Experimental animals and strains:

[0055] SPF grade BALB / C mice, female, 7 weeks old, weight 18-20g, purchased in Beijing Wirong Lihua Experimental Animal Technology Co., Ltd. (Production license number SCXK (Beijing) 2012-0001).

[0056] Vaginal Gardney is used in vaginal Gardney ATCC 14018, purchased in GDMCC, a bacteria Square Square, Guangdong Microbiology Research Institute.

[0057] Experimental schemes and groups:

[0058] Table 1. Mouse experiment group

[0059]

[0060] The mice were randomly divided into 6 groups according to the body weight, referring to Table 1, and all groups of mice were normally raised.

[0061] The bacterial vaginitis model group and intervention group need to experience infection of vagina in 5 days (0-4 days), infection (the specific operation of the infection), the sterile of vaginal Gardney with a gun 20 μl of liquid (the concentration ...

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Abstract

The invention discloses lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of the lactobacillus jensenii, and belongs to the technical field of microorganisms. The lactobacillus jensenii is screened from the vagina of a healthy woman, and the lactobacillus jensenii can remarkably relieve and improve bacterial vaginitis caused by gardnerella, regulate the level of inflammatory factors, relieve the condition that vaginal epithelial cells fall off and improve the recovery capacity of vaginal tissue. The lactobacillus jensenii can be used for preparing products for relieving bacterial vaginitis and has a wide application prospect.

Description

Technical field [0001] The present invention relates to a Jasper Lactobacillus and its application thereof that alleviate the inflammation caused by Cathopani, and belongs to the field of microbial technology. Background technique [0002] Bacterial vaginitis is the most common cause of the abnormalities of the vaginal secretion of childbearing age, characterized by vaginal stealing augantoxic bacteria and lost normal advantage bacteria, the clinical manifestation is mutually itching, burning sensation, a large number of vaginal secretions Rotten fish smell. At the same time, bacterial vaginitis is related to a wide range of health problems, including premature birth, pelvic inflammatory disease, increased susceptibility to HIV infection, and other chronic health problems. These complications are a common phenomenon in women of childbearing age, and only more than 10% of pregnant women are affected by pregnant women. At the same time, high medical expenses have also been produced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/04C12R1/225
CPCY02A50/30
Inventor 张秋香陈卫程秋涵崔树茂毛丙永唐鑫赵建新张灏
Owner JIANGNAN UNIV
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