Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of nano polymer microspheres with high fluorescence intensity

A nano-polymer, fluorescence intensity technology, applied in the direction of microsphere preparation, microcapsule preparation, etc., can solve the problems of affecting swelling efficiency and difficult penetration of dye molecules, so as to improve fluorescence intensity, good photobleaching resistance, and improve detection sensitivity Effect

Active Publication Date: 2022-04-26
SOUTHEAST UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polymer chains of the polymer microspheres are tightly entangled, and it is difficult for the dye molecules to penetrate into the microspheres, which affects the swelling efficiency.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of nano polymer microspheres with high fluorescence intensity
  • Preparation method of nano polymer microspheres with high fluorescence intensity
  • Preparation method of nano polymer microspheres with high fluorescence intensity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of nanopolymer fluorescent microspheres of the present invention comprises the following steps:

[0031] (1) Nanopolymer microspheres were prepared by soap-free emulsion polymerization: 1 mL of styrene was added to 29 mL of deionized water, and the air in the reaction device was driven away with high-purity nitrogen gas. After magnetic stirring for 0.5 h, the temperature was raised to 75 °C, and 1 mL of supernatant was added. Potassium sulfate aqueous solution (18mg / mL), after reacting for 5h, add 0.1mL of succinic acid mono[2-[(2-methyl-acryloyl)oxy]ethyl] ester for copolymerization, react for 18h, cool to room temperature, take out micro Spherical emulsion, centrifuged and washed 5 times with absolute ethanol and deionized water to obtain nano-polymer microspheres with good spherical shape and uniform particle size;

[0032] (2) Incorporate dye molecules into nanopolymer microspheres by swelling method: take 0.1g polymer microspheres and dispers...

Embodiment 2

[0034] The preparation method of nanopolymer fluorescent microspheres of the present invention comprises the following steps:

[0035] (1) Nanopolymer microspheres were prepared by soap-free emulsion polymerization: Nanopolymer microspheres were prepared by soap-free emulsion polymerization: 1 mL of styrene was added to 29 mL of deionized water, and the air in the reaction device was driven away with high-purity nitrogen. After magnetic stirring for 0.5h, the temperature was raised to 75°C, 1mL potassium persulfate aqueous solution (18mg / mL) was added, and 0.2mL lauryl methacrylate (unsaturated olefin with multiple double bonds, which can double bond to open, thereby improving the molecular chain structure and increasing the flexibility of the molecule), after another 3 hours of reaction, add 0.1 mL of succinic acid mono[2-[(2-methyl-acryloyl)oxy]ethyl] ester, and cool down after 18 hours of reaction to room temperature, take out the microsphere emulsion, and centrifuge and wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of nano polymer microspheres with high fluorescence intensity, which comprises the following steps: adding a plasticizer and a carboxyl functional monomer for copolymerization during preparation of the nano polymer microspheres to obtain polymer microspheres, and doping a fluorescent dye into the polymer microspheres by a swelling method to obtain the nano polymer fluorescent microspheres. According to the method disclosed by the invention, not only can the nano polymer microspheres with good monodispersity and clean surfaces be obtained, but also the influence of additives such as a stabilizer and a surfactant on the performance of the microspheres can be avoided; the polymer microsphere polymer chain prepared by the method has good flexibility and can be quickly opened during swelling, so that dye molecules can quickly permeate into the microspheres, and the swelling efficiency is improved; the fluorescent microspheres prepared by the method have high fluorescent molecule doping amount, so that the fluorescence intensity of the polymer microspheres is greatly improved, and the detection sensitivity is effectively improved; in addition, good photobleaching resistance is also realized.

Description

technical field [0001] The invention relates to a preparation method of nanometer polymer microspheres with high fluorescence intensity. Background technique [0002] Immunochromatography has the advantages of simplicity, speed, low cost, no need for complex equipment, and visible results. It is widely used in food safety, water quality monitoring, and medical diagnosis. However, compared with other detection technologies, such as electrochemical sensors and enzyme-linked immunosorbent assays, traditional immunochromatography methods have low sensitivity and cannot meet the growing detection requirements in various fields. The further improvement of the sensitivity of immunochromatography technology has become a bottleneck problem that needs to be solved urgently. There are four main strategies to improve the sensitivity of immunochromatography: select highly specific antibodies to increase coupling efficiency; improve test strip materials, sample addition methods, and chro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/14B01J13/20C08F212/08C08F222/26C08F220/18
CPCB01J13/14B01J13/20C08F212/08C08F222/26C08F220/1812
Inventor 王志飞杨雪邢肖肖姜勇
Owner SOUTHEAST UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com