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Laboratory nucleic acid product removing method and device

A cleaning device and laboratory technology, applied in separation methods, chemical instruments and methods, gas treatment, etc., can solve problems such as cumbersome operation, experimental failure, and weak elimination effect, and achieve improved light conversion rate, improved removal effect, and high The effect of clearance

Pending Publication Date: 2022-04-29
XIAMEN HAIHONGXING INSTR
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology also has technical problems that extremely small amounts of pollution can also cause false positives. Amplified product contamination is the most common pollution problem in PCR reactions, and severe cases may lead to the failure of the entire experiment.
[0003] At present, the methods for eliminating the pollution of amplification products are mainly to wipe or inactivate the experimental equipment with alcohol and other solvents, or hydrogen peroxide atomization and fumigation, and at the same time assist the irradiation of ultraviolet lamps, but the elimination effect of the above methods is very small , and there are technical defects such as cumbersome operation, time-consuming and labor-intensive process, and solvent residue

Method used

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  • Laboratory nucleic acid product removing method and device
  • Laboratory nucleic acid product removing method and device
  • Laboratory nucleic acid product removing method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Refer to attached figure 1 , this embodiment provides a laboratory nucleic acid product removal device, which is applied to a biological detection laboratory, and is used to reduce and eliminate aerosol pollution caused by nucleic acid and products, as well as related substances, and improve the effectiveness of laboratory test results , avoid the detection of false positive samples, and improve the continuous detection ability of the laboratory.

[0023] The device described in this embodiment includes an airtight chamber. The airtight compartment includes a detachably connected lamp bead illuminating board 1 and a compartment body 2 . When disinfecting samples, remove the lamp bead irradiation board 1, place the sample on the sample tray 4 in the chamber body 2, and then cover the lamp bead irradiation board 1 back to the top of the chamber body 2, and the edge of the lamp bead irradiation board 1 is provided with The rubber material makes it airtightly connected wit...

Embodiment 2

[0057] The present embodiment provides the device described in embodiment one to the elimination experiment of aflatoxin B1 in rice, and experimental condition is as follows:

[0058] Select 5g of mold-contaminated rice, and its aflatoxin B1 content is determined to be 30ug / kg. Spread the sample on the sample tray 4 and place it at the bottom of the airtight chamber. Select UVC lamp beads with a wavelength range of ultraviolet rays of 260-270nm and a center wavelength of 265nm. The optical power of the UVC lamp bead assembly is 13W. On the lamp bead irradiation board 1, turn on the UVC lamp bead assembly, irradiate the samples for disinfection, the irradiation distance is 5cm, collect the samples at three time points with irradiation time of 2, 5, and 10 seconds respectively, and the samples collected at each time point Sample utilizes liquid chromatography mass spectrometry to detect aflatoxin B1, and detection result is respectively 3ug / kg, 0.1ug / kg and undetected (detection...

Embodiment 3

[0060] This embodiment provides the device described in Embodiment 1 to eliminate the PCR aerosol experiment, and the experimental conditions are as follows:

[0061] Select the PCR amplification product with a concentration of 1ug / ml, place the sample collection tube at the bottom of the airtight chamber, and create an aerosol pollution model in the airtight chamber through a nebulizer. The wavelength range of ultraviolet rays is 260-270nm, and the central wavelength is 265nm UVC lamp beads, the optical power of the UVC lamp bead assembly is 13W, fixed on the lamp bead irradiation board 1, turn on the UVC lamp bead assembly, irradiate the sample to disinfect, the irradiation distance is 5cm, and the irradiation time is respectively collected. 2, 5, and 10 seconds for the samples at three time points, the CT values ​​of the samples collected at each time point detected by fluorescent quantitative PCR were 30.1, 33.3 and 39.4 (20.5 for the non-irradiated control sample), indicatin...

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Abstract

The invention discloses a laboratory nucleic acid product removing method and device, the device comprises a UVC lamp bead assembly, the UVC lamp bead assembly is used for disinfecting and killing a sample, the UVC lamp bead assembly comprises UVC lamp beads, the optical power range of the UVC lamp beads is 50-100 mW, the optical power range of the UVC lamp bead assembly is 30-100 W, the wavelength range of ultraviolet rays emitted by the UVC lamp beads is 260-270 nm, the center wavelength is 265 nm, and the UVC lamp bead assembly is used for disinfecting and killing the sample. Or the wavelength range of the ultraviolet rays emitted by the UVC lamp beads is 270-280 nm, and the central wavelength is 275 nm. The UVC lamp bead capable of generating a certain deep ultraviolet band is utilized to physically irradiate a polluted sample or formed aerosol and toxin, so that nucleic acid and product pollution can be effectively removed within a short time, the removal rate is high, and ultraviolet leakage is avoided.

Description

technical field [0001] The invention relates to the technical field of ultraviolet disinfection and sterilization, in particular to a method and device for removing nucleic acid products in a laboratory. Background technique [0002] In modern biological testing laboratories, polymerase chain reaction (PCR) is usually used to detect nucleic acids, viruses, and bacteria. This technology is an in vitro nucleic acid amplification technology developed in the 1980s. It has specific , Sensitive, efficient, simple, repeatable, easy automation and other outstanding advantages. However, this technology also has technical problems that extremely small amounts of pollution can also cause false positives. Among them, amplification product contamination is the most common pollution problem in PCR reactions, and severe cases may lead to the failure of the entire experiment. [0003] At present, the methods for eliminating the pollution of amplification products are mainly to wipe or inac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D53/00
CPCB01D53/007B01D2259/804
Inventor 张荣宝崔生辉曹进李景云
Owner XIAMEN HAIHONGXING INSTR
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