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A method for vegetative propagation of dormant buds of Acer japonica

A technology of dormant buds and white maple, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of no regeneration plant formation, lack of research, low induction rate, etc., to improve breeding efficiency and popularize Good performance and high efficiency

Active Publication Date: 2022-06-28
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research on the in vitro regeneration system of Acer japonica is relatively lacking.
Researchers such as Liu Baoguang obtained adventitious buds through callus from immature zygotic embryos of Acer japonica, and formed regenerated plants after rooting, but the induction rate was low (Liu Baoguang, Shi Ying, Journal of Plant Physiology, 2010, 46(6) :613-614); and researchers such as Gu Defeng carry out dormant bud culture in vitro, obtain germination, but do not have regeneration plant to form (Gu Defeng etc., Northern Gardening, 2010 (20): 154-155)

Method used

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  • A method for vegetative propagation of dormant buds of Acer japonica
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  • A method for vegetative propagation of dormant buds of Acer japonica

Examples

Experimental program
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Effect test

example 1

[0019] Example 1 The germination and multiplication of dormant buds of Acer japonica

[0020] In the early spring, in the Heilongjiang Forest Botanical Garden, the budded branches of the maple in the dormant period were selected, and the branches were cut into about 3.0cm budded stems, rinsed with running water for 10 minutes, and then disinfected in the ultra-clean workbench as follows: Soak in 75% alcohol for 5 minutes, soak in 5% sodium hypochlorite for 12 minutes, rinse with sterilized water for 5 times, and finally dry the surface moisture on sterile filter paper. The sterilized buds were peeled off the bud scales in the ultra-clean bench, and the cut buds were inoculated under the following culture conditions: containing 20g / L sucrose and 7.47g / L agar plant gel, and different plant growth regulators (BA, NAA 、GA 3 , KT, TDZ, IBA, ZT) cultured in DKW medium, and cultured under light. It was found that dormant buds began to germinate within 1 week, adventitious buds bega...

example 2

[0024] Example 2 Proliferation of Adventitious Buds of Acer japonica

[0025] The adventitious buds that example 1 obtains are cut into 2.0cm length, inoculate under following conditions: contain 20g / L sugar and 7.47g / L agar plant gel, and plant growth regulator (NAA, GA 3 , ZT, BA, TDZ) in the DKW medium for proliferation culture, and cultured under light. The proliferation of adventitious buds after 4 weeks of cultivation, the statistical results are shown in Table 2. The results showed: in the culture condition DKW+0.03mg / L TDZ+0.01 mg / L NAA+0.5mg / L GA 3 Under the above conditions, the induction rate of adventitious buds was the highest and the number of induced adventitious buds was more, 88.9% and 7.5, respectively, and the growth state of the seedlings was the best. Finally, this condition was determined to be the most suitable culture condition for adventitious bud proliferation.

[0026] The effect of table 2 plant growth regulators on the proliferation of adventitio...

example 3

[0028] Example 3 Adventitious shoots take root

[0029] Cut the tender adventitious buds obtained in Example 2, the length is 2.0cm, inoculate into the 1 / 2DKW medium containing IAA, IBA, NAA, 20g / L sucrose, 0.1% gac, 7.47g / L agar to induce rooting, form regeneration whole plant. After 3 weeks of cultivation, the statistical test results showed that as shown in Table 3, the rooting rate of 1 / 2DKW+0.1% activated carbon+0.5mg / L IBA+1.0mg / L IAA was higher, 50%, and the average rooting number was 4.

[0030] The influence of table 3 auxin plant growth regulator on rooting

[0031]

[0032] Note: "-" means no root build

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Abstract

A through the white cow maple ( Acer mandshuricum The invention discloses a method for asexual propagation of dormant buds, belonging to the field of forestry biotechnology. In order to solve the problem of insufficient supply of fine seedlings of Acer japonica at present, a vegetative propagation system through dormant buds was established. The main technical process is as follows: take the early spring dormant buds of the white cow maple as explants, inoculate them under sterile culture conditions with plant growth regulators after disinfection, induce their germination and adventitious bud formation, and then realize a large number of adventitious buds Propagation, and by rooting adventitious buds, a large number of neat and consistent asexual propagation seedlings are obtained, and then transplanted into the soil to grow for afforestation. The invention can overcome the limitation of the seedling output of the traditional Acer japonica seed propagation method and the problem that the excellent germplasm traits are not easy to be stably inherited, and provide technical support for the breeding of improved varieties and genetic breeding.

Description

technical field [0001] The invention relates to a method for vegetative propagation of dormant buds of Acer mandshuricum Maxim. The invention belongs to the technical field of forestry biology. Background technique [0002] White cow maple (Acer mandshuricum Maxim.), scientific name Northeast Acer, belongs to Acer family Acer, deciduous tree (Wang Xiangdong et al., Green Science and Technology, 2012 (5): 32), is famous for its bright red leaves in autumn, and is a popular species in my country. An important color-leaved tree species in Northeast China. Due to its high landscape value and ecological value, the demand for its seedlings is increasing at this stage. [0003] At present, the supply of seedlings for Acer japonica in the market is mainly based on seed propagation, but there are problems of low seed yield, small scale of seedling production and difficulty in maintaining its good woody species, so the market is in short supply of colored seedlings The status quo ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 由香玲王灵宇赵曦阳曲冠证张世凯
Owner NORTHEAST FORESTRY UNIVERSITY
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