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Peanut rhizosphere microbial strain and method for applying biological bacterial fertilizer thereof to soil improvement and remediation

A technology of microbial bacteria and strains, applied to peanut rhizosphere microbial strains and the biological fertilizer prepared by it in the field of soil improvement and restoration, can solve the problems of no medicine available for diseases, no consideration of interaction, inhomogeneity, etc.

Active Publication Date: 2022-05-06
HEBEI JINGAN BIOMASS ENERGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some excellent strains have been reported in the biological control of peanut fruit rot, but the effect of biocontrol microorganisms in the field control practice is unstable and uneven, resulting in no drug available for the disease in actual production.
This is because the biocontrol strains only consider their inhibitory effect on pathogenic bacteria, but do not consider the soil microenvironment in which they live and the interaction with other microorganisms or plants, resulting in their inability to colonize in the field, even if they are partially determined. After the colonization, the soil environment on which the pathogenic bacteria depended was not changed, nor did it promote the synergistic effect of microorganisms, crops and soil

Method used

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  • Peanut rhizosphere microbial strain and method for applying biological bacterial fertilizer thereof to soil improvement and remediation
  • Peanut rhizosphere microbial strain and method for applying biological bacterial fertilizer thereof to soil improvement and remediation
  • Peanut rhizosphere microbial strain and method for applying biological bacterial fertilizer thereof to soil improvement and remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation and purification of strains

[0046] Take 10g soil samples from peanut rhizosphere soil samples in 3 natural villages including Chizhi Village, Cheng'an Town, Xinle City, Shijiazhuang City, Hebei Province, the Farm of Hebei Science and Technology Teachers College, Changli County, Qinhuangdao City, Hebei Province, and Baituozi, Changli County, and put them in a 250mL conical flask , add 90mL sterile water, and put three glass beads, shake in a shaker at 220rpm for 30min, and let it stand for 30sec to prepare 10 -1 Soil dilution; pipette from 10 -1 Aspirate 1mL from the soil dilution solution, add it to a large test tube filled with 9mL sterile water, and mix well to prepare 10 -2 Soil dilution; again from 10 -2 Aspirate 1mL from the soil dilution solution, add it into a large test tube filled with 9mL sterile water, and mix well to obtain 10 -3 Soil dilution, and so on, were prepared to obtain 10 -3 , 10 -4 , 10 -5 Soil dilutions were subsequently pipette...

Embodiment 2

[0048] The identification of embodiment 2 bacterial strains

[0049] (1) Refer to the "Common Bacteria System Identification Manual" for morphological identification: GF-32a is a Gram-negative bacterium with round colonies and protrusions, such as half a glass ball; colorless and transparent, smooth surface, viscous, large elasticity, Can be drawn into filaments ( figure 1 ), thick and long rod-shaped, thick capsule, large spore, oval mesophyte, cell size of 0.9~1.0μm32.5~4.0μm( figure 2 ). GD-16 is a Gram-positive bacterium, the colony is round, milky white, smooth, viscous and easy to pick up, opaque, with neat edges, rod-shaped, multiple double rods, spores, 1.32-1.7033.73-6.60 μm.

[0050] After GF-32a was activated on silicate medium and GD-16 strain was activated on LB medium, the physiological and biochemical characteristics were analyzed and determined by Mérieux VITEK-2Compact automatic bacterial identification and analysis system and biochemical identification ca...

Embodiment 3

[0058] Example 3 Preliminary Verification of Dissolving Phosphorus, Potassium and Nitrogen Fixation Ability

[0059] In the ultra-clean operation bench, the bacterium solution of the GF-32a bacterial strain was placed on the silicate medium (the formula is as follows: sucrose 5.0g, Na 2 HPO 4 2.0g, MgSO 4 0.5g, CaCO 3 0.1g, 0.5% FeCl 3 2 h 2(O 1.0mL, agar 20.0g, distilled water constant volume to 1000.0mL, pH7.2-7.4) for activation, the GD-16 and GF-3 strains were in LB medium (the formula is as follows: yeast extract powder 5.0g, peptone 10.0 g, NaCl 10.0g, agar 20.0g, distilled water 1000.0mL, pH 7.0) for activation, use a 1mL tip of a pipette gun to make bacterial cakes of the same size on the activated culture, and then separate the bacterial cakes Put it on the potassium solution medium (GF-32a, the medium formula is as follows: glucose 5g, ammonium sulfate 0.5g, yeast powder 0.5g, magnesium sulfate 0.3g, disodium hydrogen phosphate 2g, ferrous sulfate 0.03g, mang...

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Abstract

The invention provides a flowering plant rhizosphere microbial strain and a method for applying a biological bacterial fertilizer of the flowering plant rhizosphere microbial strain to soil improvement and remediation, and relates to the technical field of soil improvement. The invention comprises a bacterial strain which has an inhibition effect on pathogenic bacteria of peanut fruit rot, a bacterial strain which has the capabilities of dissolving phosphorus, dissolving potassium, fixing nitrogen, adjusting pH and degrading autotoxic substances of peanuts, and a bacterial strain which secretes a plant growth regulator. When the strains are compounded and applied to peanut planting, the obvious growth promoting, disease preventing and yield increasing effects are achieved. Meanwhile, according to the biological bacterial fertilizer prepared from the peanut rhizosphere microbial bacterial system, the microbial bacterial system, organic matter and inorganic compound fertilizer are applied in a matched mode to prevent and treat peanut fruit rot, and the yield can be increased through the synergistic interaction effect of growth-promoting bacteria and the growth-promoting effect of the biological organic fertilizer; and peanut fruit rot can be prevented and controlled through the synergistic effect of the biocontrol strain and the growth-promoting strain, so that the yield is increased.

Description

technical field [0001] The invention belongs to the technical field of soil improvement, and in particular relates to a method for soil improvement and restoration of a peanut rhizosphere microbial strain and the biological bacterial fertilizer prepared therefrom. Background technique [0002] Peanut is one of the main oil crops and economic crops in my country. Peanut fruit rot is a kind of soil-borne disease that generally occurs in the world. It has been reported in North America, Australia, Asia, Africa and other places. In recent years, peanut fruit rot has occurred in major peanut producing areas such as Hebei, Henan, Shandong, and Liaoning, and has a tendency to increase year by year. It has become a major disease in major peanut producing areas in my country. Peanut fruit rot can cause about 30% of peanut pods to rot, which seriously threatens the healthy development of peanut industry. [0003] Heavy cropping (continuous cropping obstacle) can aggravate the occurre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05D1/00C05G3/60C05G3/80C09K17/14C12R1/07
CPCC12N1/20C05D1/00C05G3/60C05G3/80C09K17/14C09K2101/00C05D1/02C05D3/02C05F3/00C05F5/00C05F11/02C05F11/08
Inventor 孙伟明冯丽娜邢单润胡朋举焦镇李步阳宋亚辉
Owner HEBEI JINGAN BIOMASS ENERGY TECH CO LTD
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