Peanut rhizosphere microbial strain and method for applying biological bacterial fertilizer thereof to soil improvement and remediation
A technology of microbial bacteria and strains, applied to peanut rhizosphere microbial strains and the biological fertilizer prepared by it in the field of soil improvement and restoration, can solve the problems of no medicine available for diseases, no consideration of interaction, inhomogeneity, etc.
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Embodiment 1
[0045] Isolation and purification of strains
[0046] Take 10g soil samples from peanut rhizosphere soil samples in 3 natural villages including Chizhi Village, Cheng'an Town, Xinle City, Shijiazhuang City, Hebei Province, the Farm of Hebei Science and Technology Teachers College, Changli County, Qinhuangdao City, Hebei Province, and Baituozi, Changli County, and put them in a 250mL conical flask , add 90mL sterile water, and put three glass beads, shake in a shaker at 220rpm for 30min, and let it stand for 30sec to prepare 10 -1 Soil dilution; pipette from 10 -1 Aspirate 1mL from the soil dilution solution, add it to a large test tube filled with 9mL sterile water, and mix well to prepare 10 -2 Soil dilution; again from 10 -2 Aspirate 1mL from the soil dilution solution, add it into a large test tube filled with 9mL sterile water, and mix well to obtain 10 -3 Soil dilution, and so on, were prepared to obtain 10 -3 , 10 -4 , 10 -5 Soil dilutions were subsequently pipette...
Embodiment 2
[0048] The identification of embodiment 2 bacterial strains
[0049] (1) Refer to the "Common Bacteria System Identification Manual" for morphological identification: GF-32a is a Gram-negative bacterium with round colonies and protrusions, such as half a glass ball; colorless and transparent, smooth surface, viscous, large elasticity, Can be drawn into filaments ( figure 1 ), thick and long rod-shaped, thick capsule, large spore, oval mesophyte, cell size of 0.9~1.0μm32.5~4.0μm( figure 2 ). GD-16 is a Gram-positive bacterium, the colony is round, milky white, smooth, viscous and easy to pick up, opaque, with neat edges, rod-shaped, multiple double rods, spores, 1.32-1.7033.73-6.60 μm.
[0050] After GF-32a was activated on silicate medium and GD-16 strain was activated on LB medium, the physiological and biochemical characteristics were analyzed and determined by Mérieux VITEK-2Compact automatic bacterial identification and analysis system and biochemical identification ca...
Embodiment 3
[0058] Example 3 Preliminary Verification of Dissolving Phosphorus, Potassium and Nitrogen Fixation Ability
[0059] In the ultra-clean operation bench, the bacterium solution of the GF-32a bacterial strain was placed on the silicate medium (the formula is as follows: sucrose 5.0g, Na 2 HPO 4 2.0g, MgSO 4 0.5g, CaCO 3 0.1g, 0.5% FeCl 3 2 h 2(O 1.0mL, agar 20.0g, distilled water constant volume to 1000.0mL, pH7.2-7.4) for activation, the GD-16 and GF-3 strains were in LB medium (the formula is as follows: yeast extract powder 5.0g, peptone 10.0 g, NaCl 10.0g, agar 20.0g, distilled water 1000.0mL, pH 7.0) for activation, use a 1mL tip of a pipette gun to make bacterial cakes of the same size on the activated culture, and then separate the bacterial cakes Put it on the potassium solution medium (GF-32a, the medium formula is as follows: glucose 5g, ammonium sulfate 0.5g, yeast powder 0.5g, magnesium sulfate 0.3g, disodium hydrogen phosphate 2g, ferrous sulfate 0.03g, mang...
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