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Detection system and detection method for instant blood coagulation function detection

A detection system and functional detection technology, applied in the field of biomedical engineering, can solve the problems of microfluidic technology, such as low sample consumption, integration level, limited practical application, and dependence, and achieve blood sample with less reagent volume, short detection time, and good correlation Effects of Sex and Consistency

Pending Publication Date: 2022-05-06
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Microfluidic technology has the advantages of small size, less sample consumption, and high integration, which provide a powerful tool for instant diagnostic coagulation analysis, but currently reported microfluidic coagulation devices use droplet microfluidics, acoustic waves, optics, micropores Arrays, mechanical resonance, etc., the realization of which depends on complex instruments such as syringe pumps, acoustic sensors, and fluorescence microscopes, which limits their practical applications

Method used

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  • Detection system and detection method for instant blood coagulation function detection
  • Detection system and detection method for instant blood coagulation function detection
  • Detection system and detection method for instant blood coagulation function detection

Examples

Experimental program
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Embodiment 1

[0040] Example 1 Preparation of digital microfluidic chip based on PCB board

[0041] Such as figure 1 As shown, a digital microfluidic chip based on a PCB adopts a single-plate or double-plate structure; the single-plate structure includes a base, an electrode layer embedded on the base, and an interlayer covering the electrode layer from bottom to top. The electric layer, the hydrophobic layer on the dielectric layer and the zero electrode in the hydrophobic layer; the double-plate structure is composed of the upper and lower plates, and there is a gap between the upper and lower plates; the lower plate is from bottom to top, including the base, The electrode layer embedded on the substrate, the dielectric layer covering the electrode layer, the hydrophobic layer on the dielectric layer and the zero electrode in the hydrophobic layer are composed; the upper plate is from bottom to top, including the hydrophobic layer and ITO glass. Use the PCB board as the substrate, immers...

Embodiment 2

[0042] Example 2 Detection system and detection method for blood coagulation function detection

[0043] Combining the digital microfluidic chip based on the PCB board of the above-mentioned Example 1 with the image collection and processing platform to build a detection system for blood coagulation function detection, the blood droplets are driven by voltage to move back and forth on the electrode array, and the blood flow is monitored at the same time. The blood coagulation analysis is carried out according to the exercise situation, and the image processing and data output are carried out through MATLAB 2020a, ORIGIN 2020 and other software.

[0044] The detection system used for blood coagulation function detection is as follows: figure 2 As shown, it includes a personal computer, a single-chip microcomputer control module, a high-voltage drive module, a high-voltage amplifier, a digital microfluidic chip based on a PCB, and a digital microscope. The output end of the pe...

Embodiment 3

[0052] Example 3 Optimization and verification of blood coagulation function test method

[0053] Based on the detection system and detection method for coagulation function detection described in the above-mentioned embodiment 2. The hardware part of the detection system and the biochemical parameters of the analysis experiment were optimized, and the influence of driving voltage, refresh rate, temperature, coagulant and anticoagulant on the coagulation experiment was explored.

[0054] 1. Optimization of test parameters

[0055] (1) Regarding the hardware settings of the digital microfluidic chip on the PCB, the voltage of the electrodes and the refresh rate of the voltage signal are the main parameters that affect the measurement. The magnitude of the voltage will affect the strength of the electric field, thereby affecting the driving force of the blood droplet movement, which may have a certain impact on the coagulation experiment. The voltage was adjusted systematicall...

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Abstract

The invention discloses a detection system and a detection method for instant blood coagulation function detection. According to the detection system, blood liquid drops are driven according to the dielectric wetting effect, and a blood sample is tested and analyzed. The invention provides a novel method for clinically detecting the blood coagulation function for the first time, blood liquid drops are driven through a digital micro-fluidic technology of a PCB (Printed Circuit Board), a blood sample is dynamically monitored, the speed and the length of the liquid drops are respectively calculated, and the blood coagulation time and the clot rigidity are calculated; the obtained experimental result has correlation and consistency with the measurement result of a commercial standard instrument, and the method is proved to have a great application prospect. Besides, the detection system has the advantages of miniaturization, portability, low cost, small blood sample reagent amount, short detection time and the like, can be used for real-time diagnosis occasions such as perioperative period nursing, severe nursing, wound first-aid sites and the like, and has a great application prospect in real-time diagnosis (POCT) occasions.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering. Specifically, it relates to a detection system and a detection method for instant blood coagulation function detection. Background technique [0002] Coagulation monitoring is important for the diagnosis of hemorrhagic and thrombotic disorders, perioperative care, emergency care, etc. At present, clinical coagulation testing mainly focuses on four time-based coagulation items, which mainly focus on two aspects for evaluation. On the one hand, it is based on the end point detection of coagulation time, such as prothrombin time (prothrombin time, PT), thrombin time (thrombin time, TT), activated partial prothrombin time (activated partial thromboplastintime, APTT). On the other hand, it involves biochemical functions and measuring the concentration of related proteins, such as coagulation factors (Clotingfactors), fibrinogen (fibrinogen, FIB), D-dimer (dimer). [0003] Although th...

Claims

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Application Information

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IPC IPC(8): G01N33/86B01L3/00
CPCG01N33/86B01L3/5027B01L2300/0645B01L2300/166B01L2300/12B01L2200/10B01L2200/027B01L2300/0887
Inventor 李自达李东豪柴语鹃刘昕宇郑卫东
Owner SHENZHEN UNIV