Functional flora for producing kaempferol as well as preparation and application of functional flora

A technology of functional bacteria and kaempferol, applied in biochemical equipment and methods, bacteria, fungi, etc., can solve the problems of inability to directly utilize natural raw materials, high application cost, and high complexity of fermentation process, and achieve the promotion of industrialization development and cost. The effect of low and broad application prospects

Active Publication Date: 2022-05-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the entire process avoids the use of fossil fuel derivatives, the fermentation process is still highly complex and cannot directly use natural raw materials. It is necessary to extract specific sugars from plants first, and then pass through a series of fermentation processes, and the application cost is relatively high.

Method used

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  • Functional flora for producing kaempferol as well as preparation and application of functional flora
  • Functional flora for producing kaempferol as well as preparation and application of functional flora
  • Functional flora for producing kaempferol as well as preparation and application of functional flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preliminary screening of functional microorganisms

[0027] In order to solve the problems of serious pollution and high cost in the production process of kaempferol, it is first necessary to select a suitable strain. According to the existing research basis, the Fusarium spp. producing kaempferol was determined as the main fermentation strain. The co-fermentation strain mainly considers that it has the ability to hydrolyze the cell wall, change the permeability of the cell membrane, and withstand pressure (adapt to harsh fermentation conditions), can antagonize chloramphenicol, can produce a large amount of biofilm, and can coexist with Fusarium. And the growth and metabolism are strong. The main focus has been on various Bacillus species and Rhizobium species. Further, to reduce the antagonism between strains and exert their synergistic effect, mainly select Bacillus and Rhizobium to co-ferment together. The co-fermentation effects of different combinati...

Embodiment 2

[0037] Embodiment 2: Composition and source of functional microorganism

[0038] The microorganisms involved in the present invention are all purchased from various bacterial species preservation banks, respectively Fusarium solani ATCC 36031, Bacillus velezensis DSM 28326, Bacillus pumilus ATCC 7061, Bacillus subtilis Bacillus subtilis ATCC6051, Rhizobium alamii LMG 24466 and Rhizobium mesosinicum LMG 24135.

[0039] The strains purchased from the American Type Culture Collection (ATCC) in May 2018 include: Fusarium solani ATCC 36031, Bacillus pumilus ATCC 7061, and Bacillus subtilis ATCC 6051.

[0040] The strains purchased from the German Microorganism and Cell Collection (DSM) in October 2017 include: Bacillus velezensis DSM 28326.

[0041] Purchased from the National Type Culture Collection (NTCC) in October 2017, including: Rhizobium alamii LMG 24466 and Rhizobium mesosinicum LMG 24135.

Embodiment 3

[0042] Embodiment 3: the cultivation method of each microbial strain

[0043] Bacillus Velez and Rhizobium sinensis were inoculated into the culture medium to obtain seed solution. The culture conditions were: temperature 26°C, rotation speed 160rpm, initial pH value 7.0, dark culture to logarithmic phase. For the entire fermentation process of the Fusarium, the separate culture conditions in the early stage are that the temperature is 26°C, the rotation speed is 160rpm, the initial pH value is 6.5, and the dark culture time is 3 days; The medium used was all potato dextrose medium supplemented with 0.12g / L chloramphenicol.

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Abstract

The invention discloses a functional flora for producing kaempferol as well as preparation and application thereof, and belongs to the technical field of kaempferol production through microbial fermentation. According to the method, potatoes and glucose are taken as substrates, the cell membrane permeability is changed by utilizing bacillus, the mediation effect of rhizobium is utilized, and the bacillus and fusarium are co-fermented to produce kaempferol. The production method is simple, low in production cost, environment-friendly and capable of producing various other flavonoid compounds at high yield. When the method is used for producing the kaempferol, the yield of the kaempferol of the fusarium is obviously improved. According to the method, the problems of serious pollution and high cost in the kaempferol production process are solved, a more natural and sustainable substitute can be provided for consumers, the industrial development of a biological fermentation technology is promoted, and the large-scale production of kaempferol can be realized.

Description

technical field [0001] The invention relates to a functional bacterial group producing kaempferol and its preparation and application, and belongs to the technical field of kaempferol produced by microbial fermentation. Background technique [0002] Flavonoids are currently the focus of research and development in the field of medicine and nutritional products at home and abroad. The related ingredients of this type of compound can be applied in a wide range of directions, including skin, inflammation, immune and other product formulations. According to the market data released by InsightSLICE, by 2031, the global market share of flavonoids is expected to achieve a compound annual growth rate of 5.5%, and the market size will reach US$1.45 billion. [0003] Kaempferol is a kind of polyol hydroxyflavonoid compound widely present in various plants, and has various pharmacological activities such as anticancer, antioxidation, lowering blood pressure, lowering blood fat, and an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P17/06C12R1/07C12R1/41C12R1/77
CPCC12N1/20C12N1/14C12P17/06
Inventor 刘学端张双飞梁伊丽孙崇然周瑾张国庆
Owner CENT SOUTH UNIV
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