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Selenium-rich peptide with high antioxidant activity and application thereof

An oxidation-active, selenium-enriched technology, applied in the field of anti-oxidation, can solve problems such as research needs to be further deepened, and achieve the effect of high selenium content

Active Publication Date: 2022-05-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on Moringa seed protein peptides is mostly carried out in the form of mixtures, and the research on its peptide composition, amino acid sequence and activity mechanism needs to be further studied
At present, there is a patent for a natural oligopeptide with protective effect on liver cell oxidative damage, which discloses a polypeptide with protective effect on cell damage isolated from Moringa oleifera seed, but it is not a selenium-rich polypeptide

Method used

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  • Selenium-rich peptide with high antioxidant activity and application thereof
  • Selenium-rich peptide with high antioxidant activity and application thereof
  • Selenium-rich peptide with high antioxidant activity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The separation and purification of embodiment 1 selenium-enriched peptide

[0042] The DPPH and ABTS free radical scavenging rate and selenium content of the F1-3 fraction separated from the selenium-enriched Moringa oleifera seed protein hydrolyzate were determined. The test process and results are as follows:

[0043] (1) DPPH free radical scavenging rate

[0044] Testing process:

[0045] Add 100 μL of sample solution to 100 μL of 0.15 mM DPPH solution. The mixture was then left at room temperature in the dark for 30 minutes. Measure the absorbance of the 96-well plate at 517 nm using a microplate reader. The calculation formula of DPPH free radical scavenging rate is:

[0046] DPPH free radical scavenging rate (%)=[1-(A 样品 -A 空白 ) / A 对照 ]×100 (1)

[0047] A 样品 : Absorbance of adding 100 μL sample solution to 100 μL DPPH solution;

[0048] A 空白 : Absorbance of adding 100 μL ethanol to 100 μL sample solution;

[0049] A 对照 : Absorbance of 100 μL DPPH soluti...

Embodiment 3

[0072] Antioxidant activity assay of embodiment 3 selenium-enriched peptides

[0073] The antioxidant activities of the two selenium-rich peptides obtained in Example 2 were determined.

[0074] (1) Toxic effect (MTT) detection of selenium-enriched peptides on HepG2 cells

[0075] HepG2 cell culture: 10% FBS mixed with DMEM medium, the cells were placed in 5% CO 2 Cultured in a cell culture incubator. When the HepG2 cells adhered to the wall and fused into a dense monolayer and the cell coverage reached more than 80%, they were digested and passaged with 0.25% trypsin solution or carried out to the next step.

[0076] Toxicity detection of selenium-rich peptides on HepG2 cells: 1×10 5 cells / mL of HepG2 cells were inoculated in 96-well plates (6 replicate wells for each group), set up control group and test group, added 100 μL cell suspension to the control group and test group, cultured for 24 hours, and the cells adhered to the wall, and the control group was added Add 10...

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Abstract

The invention relates to a selenium-rich peptide with high antioxidant activity and application thereof. The selenium-rich peptide has the following amino acid sequence: FLML or LAMMVL, sulfur element in methionine M in the FLML is substituted by selenium, and the selenium-rich peptide is marked as FLSeML; the sulfur element in the first methionine M in the LAMMVL is substituted by selenium. The selenium-rich peptide with high antioxidant activity is separated and purified from the selenium-rich moringa seed protein powder, and the selenium-rich peptide has high selenium content, high antioxidant activity and cell damage protection effect, and can block the interaction of Keap1-Nrf2.

Description

technical field [0001] The invention belongs to the field of anti-oxidation technology, and in particular relates to a selenium-rich peptide with high anti-oxidation activity and an application thereof. Background technique [0002] Selenium (Se) is one of the micronutrients necessary to maintain human and animal health, and has important physiological functions, including anti-oxidation, anti-cancer, anti-fatigue and immune regulation. It is estimated that 1 billion people in the world have a series of diseases caused by insufficient selenium intake. Studies have found that natural organic selenium has more advantages than inorganic selenium, such as low toxicity, high bioavailability and various physiological activities. Therefore, in recent years, there have been more and more studies on natural selenium-enriched products, including selenium-enriched rice, selenium-enriched green tea, selenium-enriched Agaricus bisporus, and selenium-enriched yeast. [0003] Moringa bel...

Claims

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Application Information

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IPC IPC(8): C07K5/107C07K7/06A61K38/08A61K38/07A61K8/64A61K8/23A61Q19/08A61P39/06A23L33/18
CPCC07K5/1016C07K7/06A61K8/645A61K8/23A61Q19/08A61P39/06A23L33/18A61K38/00A61K2800/78A23V2002/00A23V2200/30A23V2250/55
Inventor 苗建银陈冰冰梁兴唐尹艳镇
Owner SOUTH CHINA AGRI UNIV
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