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Signal turnover type photoelectrochemical biosensor for detecting cancer markers as well as preparation method and application of signal turnover type photoelectrochemical biosensor

A biosensor, photoelectrochemical technology, applied in the biological field, can solve the problems of reduced photoelectric conversion efficiency, increased electron transfer path, toxic photostability, etc., and achieves the effect of improving the inversion efficiency, shortening the electron transfer path, and having a good application prospect.

Pending Publication Date: 2022-05-13
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the core element of the photocurrent direction inversion system, the photoelectric active material directly affects its inversion performance. The two photoelectric active materials in the existing inversion system usually require biological components (such as antibodies, nucleic acids, polypeptides) to be connected without direct contact, resulting in electron Increased transfer path, reduced photoelectric conversion efficiency
At the same time, the inversion systems based on cadmium sulfide, cadmium telluride, and copper oxide also limit their practical applications due to toxicity or poor photostability.

Method used

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  • Signal turnover type photoelectrochemical biosensor for detecting cancer markers as well as preparation method and application of signal turnover type photoelectrochemical biosensor
  • Signal turnover type photoelectrochemical biosensor for detecting cancer markers as well as preparation method and application of signal turnover type photoelectrochemical biosensor
  • Signal turnover type photoelectrochemical biosensor for detecting cancer markers as well as preparation method and application of signal turnover type photoelectrochemical biosensor

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Embodiment 1

[0055] see figure 1 , Embodiment 1 of the present invention provides a signal inversion photoelectrochemical biosensor for detecting cancer markers, the sensor is mainly prepared by the following steps, the preparation steps include preparing indium tin oxide / titanium dioxide electrodes, preparing indium tin oxide / titanium dioxide / Single-stranded auxiliary probe electrode, preparation of conjugate Ab 1 -Fe 3 o 4 @Au-DNA, preparation of conjugate Ab 2 -AuNPs-DNA, construction of intermediate probes and preparation of sensors and other steps.

[0056] 1. Preparation of Indium Tin Oxide / Titanium Dioxide Electrode

[0057] will be like figure 2 The metal-organic framework material MIL-125 shown in A was carbonized in a tube furnace at 380 °C for 4 h to obtain the following figure 2 Titanium dioxide polyhedron shown in B; disperse the titanium dioxide polyhedron in ultrapure water to prepare a suspension of titanium dioxide polyhedron with a concentration of 1 mg / mL; add ...

Embodiment 2

[0103] This embodiment provides an application of the biosensor for detecting cancer markers provided in Example 1 in detecting cancer markers.

[0104]Specifically, during the preparation process of the above-mentioned biosensor provided in Example 1, the adsorption reaction time of the single-stranded auxiliary probe on the indium tin oxide / titanium dioxide electrode was 90 min, and the carbon nitride quantum dots on the indium tin oxide / titanium dioxide / single The adsorption time on the chain-assisted probe / intermediate probe electrode was 60 min; the biosensor was placed in 0.1 M ascorbic acid in Tril-HCl buffer solution (pH 7.4, 0.1 M), and a bias voltage of -0.2 V was applied for Photoelectrochemical test, detect the photocurrent signal of sCD146 at the concentration of 10, 100, 200, 1000, 10000, 50000, 100000, 1000000 and 5000000 fg / mL respectively; construct the linearity between the concentration of sCD146 and the photocurrent signal according to the detection results ...

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Abstract

The invention provides a preparation method of a signal turnover type photoelectrochemical biosensor for detecting cancer markers. The preparation method comprises the following steps: preparing an indium tin oxide / titanium dioxide electrode, preparing an indium tin oxide / titanium dioxide / single-chain auxiliary probe electrode, preparing a conjugate Ab1-Fe3O4 (at) Au-DNA, preparing a conjugate Ab2-AuNPs-DNA, constructing an intermediate probe and preparing the sensor. The invention also provides the biosensor prepared by the method and application thereof. The biosensor for detecting the cancer marker is based on the organic combination of a direct contact type photocurrent direction overturning strategy, a targeted induction three-dimensional dual-support DNA walker circulation signal amplification technology and a metal organic framework material adsorption characteristic, and photocurrent signal overturning design is carried out, so that the biosensor can be used for detecting the cancer marker. The biosensor can realize environment-friendly detection of cancer markers, and has the advantages of high sensitivity, high selectivity, good reproducibility, high stability and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a signal flipping photoelectrochemical biosensor for detecting cancer markers and a preparation method and application thereof. Background technique [0002] Soluble CD146 (sCD146) is closely related to the occurrence and development of cancer. As a molecular marker, it plays an important role in the early diagnosis and treatment of cancer. The advantages of photoelectrochemical biosensors in detecting molecular markers have attracted much attention, but most of the reported sensors are constructed based on a unidirectional signal response strategy, which is not conducive to eliminating the adverse effects of interfering substances in actual samples on the detection anti-interference ability and sensitivity , false positive or false negative errors may occur, which seriously limits its application and development in the analysis and detection of sCD146. [0003] Signal inversion typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 杨瑞英蒋桂花刘慧敏刘利娥阿有梅吴拥军
Owner ZHENGZHOU UNIV
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