Method for removing DEHP in landfill leachate
A technology for landfill leachate and suspension, which is applied in chemical instruments and methods, contaminated groundwater/leachate treatment, water/sludge/sewage treatment, etc. problems, to achieve a practical effect
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Embodiment 1
[0038] Setting of Optimum Hydraulic Parameters of A / A / O Process
[0039] A: DEHP removal rate under different hydraulic retention time conditions
[0040]The A / A / O reactor consists of a 6-L anaerobic tank (A1), a 6-L anoxic tank (A2), a 24-L aerobic tank (O) and a 25-L sedimentation tank, all Made of plexiglass. The sludge is returned to the anaerobic tank from the bottom of the sedimentation tank by a peristaltic pump. The mixed solution is circulated from the aerobic tank to the anoxic tank by a peristaltic pump. The mixed liquor suspended solids (MLSS) in the A / A / O reactor was 3000 mg / L. The entire A / A / O reactor is placed in a constant temperature room at 25°C. The ratio of hydraulic retention time in anaerobic pool, anoxic pool and aerobic pool is 1:1:4. The dissolved oxygen concentrations in the anaerobic pool, anoxic pool, and aerobic pool are controlled at 0.1-0.2 mg / L, 0.2-0.6 mg / L and 6-7.5 mg / L, respectively.
[0041] In the specific implementation, a certain a...
Embodiment 2
[0054] Under the optimal hydraulic parameters of the A / A / O process set in Example 1, the preparation and dosage of microbial suspension were carried out
[0055] Isolation, cultivation and identification of A-degrading dominant bacteria
[0056] Separation: The source of separation is sludge from the sedimentation tank of a landfill leachate treatment system, and the inorganic salt medium (g / L) is specifically: K 2 HPO 4 ·3H 2 O 0.4; (NH 4 ) 2 HPO 4 0.5; MgSO 4 ·7H 2 O 0.05; CaCl 2 0.1; FeCl 3 ·6H 2 O 0.01; agar 18.0; pH=7.4. Sterilize with damp heat at 121°C for 30 minutes. Take 1g of sludge, dissolve it in 40mL sterile water, and oscillate evenly. Take 1 mL of the above solution and inoculate it on 15 mL of inorganic salt plate medium by plate coating method. The inorganic salt medium uses DEHP as the only carbon source and energy source (100-500mg / L). After 7 days of dark culture in an incubator at 30°C, the well-developed colonies were picked out and inocula...
Embodiment 3
[0064] On the basis of Example 2, the preparation and addition of immobilized microorganisms
[0065]Prepare a mixed solution of sodium alginate with a mass fraction of 4%, polyvinyl alcohol with a mass fraction of 4%, and activated carbon with a mass fraction of 1% with distilled water, wherein the activated carbon is used to increase the permeability of the immobilized particles, and the mixed solution is used as Immobilize the embedding agent, mix the immobilized embedding agent with the microbial suspension prepared in Example 2 at a volume ratio of 1:1 to obtain a mixed microbial embedding agent, absorb the mixed microbial embedding agent and add to In calcium chloride with a mass fraction of 5%, cross-link for 5 hours to make immobilized microorganisms with a diameter of about 4mm. The prepared immobilized microorganisms were added to the aerobic tank of the A / A / O reactor in stable operation in Example 2, and the dosage of the immobilized microorganisms was 15% of the ef...
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