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Spinning labeled starch and preparation method thereof

A technology of spin labeling and starch, applied in the field of starch modification, can solve problems such as destroying the original conformation of molecules, and achieve the effects of easy operation, simple process route and stable chemical structure

Pending Publication Date: 2022-05-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only nuclear magnetic resonance, atomic force microscopy, and single-molecule force spectroscopy can study starch molecules at the molecular level, but these methods must deal with starch molecules and destroy the original conformation of the molecules.

Method used

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  • Spinning labeled starch and preparation method thereof
  • Spinning labeled starch and preparation method thereof
  • Spinning labeled starch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 2.0 g of corn starch and add 25 mL of anhydrous DMSO to dissolve at 25 ° C for 10 h to prepare a starch solution for later use. Dissolve 1 g of hexamethyl diisocyanate and 50 mg of 4-amino Tempo in 10 mL of anhydrous DMSO, and after continuous stirring for 2 h, mix it with the above starch solution and continue stirring for 24 h to obtain a mixed sample.

[0040] Use a dialysis bag with a molecular weight of 8000-16000 to hold the mixed sample and seal it, then submerge the dialysis bag into a 30% volume fraction of ethanol solution for 3 times of dialysis, each dialysis for 4 to 5 hours to remove unreacted 4-amino Tempo, HDI and DMSO molecular. After dialysis, the mixed samples were taken out and freeze-dried, pre-frozen in a -20°C refrigerator for 12 hours, then taken out and dried in a freeze dryer for 48 hours to obtain the desired spin-labeled cornstarch.

[0041] Take 50 mg of spin-labeled cornstarch in a quartz tube for electron paramagnetic resonance (EPR)...

Embodiment 2

[0044] 2.0 g of amylose was added to 35 mL of anhydrous DMSO and dissolved at 25 °C for 10 h to prepare a starch solution for later use. Dissolve 1 g of hexamethyl diisocyanate and 10 mg of 4-amino Tempo in 10 mL of DMSO, and after continuous stirring for 2 h, mix it with the above starch solution and continue stirring for 24 h to obtain a mixed sample.

[0045] Use a dialysis bag with a molecular weight of 8000-16000 to hold the mixed sample and seal it, then submerge the dialysis bag into a 30% volume fraction of ethanol solution for 3 times of dialysis, each dialysis for 4 to 5 hours to remove unreacted 4-amino Tempo, HDI and DMSO molecular. After the dialysis, the mixed sample was taken out and freeze-dried. It was pre-frozen in a -20°C refrigerator for 12 hours, and then taken out and dried in a freeze dryer for 48 hours to finally obtain the desired spin-labeled amylose.

[0046] Take 50mg of spin-labeled amylose in a quartz tube for electron paramagnetic resonance (EPR...

Embodiment 3

[0049] 2.0 g of wheat starch was added to 35 mL of anhydrous DMSO and dissolved at 25 °C for 10 h to prepare a starch solution for later use. Dissolve 1 g of hexamethyl diisocyanate and 80 mg of 4-amino Tempo in 10 mL of anhydrous DMSO, and after continuous stirring for 2 h, mix it with the above starch solution and continue stirring for 24 h to obtain a mixed sample.

[0050]Use a dialysis bag with a molecular weight of 8000-16000 to hold the mixed sample and seal it, then submerge the dialysis bag into a 30% volume fraction of ethanol solution for 3 times of dialysis, each dialysis for 4 to 5 hours to remove unreacted 4-amino Tempo, HDI and DMSO molecular. After the dialysis, the mixed samples were taken out and freeze-dried. They were pre-frozen in a -20°C refrigerator for 12 hours, then taken out and dried in a freeze dryer for 48 hours to finally obtain the desired spin-labeled wheat starch.

[0051] Take 50 mg of spin-labeled wheat starch in a quartz tube for electron p...

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Abstract

The invention relates to the technical field of starch modification, in particular to a method for self-spinning labeling of starch molecules and self-spinning labeling starch prepared through the method. According to the method, a spin marker with a spin characteristic is combined with starch molecules by a covalent binding method, so that the starch molecules have the spin characteristic; and the spin marker is a 4-amino-2, 2, 6, 6-tetramethyl piperidine oxygen free radical. The structural formula of the prepared spin-labeled starch is as follows: nitroxide free radicals with a spin characteristic are labeled on the starch in a covalent binding manner, and the labeled starch molecules have the spin characteristic in a magnetic field, so that the conformation and the structure of the starch molecules can be further analyzed under the condition of not damaging a starch chain structure.

Description

technical field [0001] The invention relates to the technical field of starch modification, in particular to a method for spin-labeling starch molecules and the spin-labeled starch prepared by the method. [0002] technical background [0003] Starch is a macromolecular carbohydrate, which is not only the source of human energy intake, but also one of the main raw materials of renewable materials. Starch is one of the most common natural materials in life. Due to its advantages of high yield, low cost and natural degradation, it is widely used in food, paper, pharmaceutical and other industries. The study of starch molecules is closely related to the progress and development of human production and life. [0004] At present, the research methods of starch mainly include differential scanning calorimetry, confocal laser scanning microscope, small angle x-ray scattering method, etc. These methods usually only work at the granular level, with certain limitations. Only methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B31/00C08B33/00
CPCC08B31/00C08B33/00
Inventor 杜先锋梅丽萍余谢天林丽李圆圆
Owner ANHUI AGRICULTURAL UNIVERSITY
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