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Method for detecting content of heterotrophic bacteria

A detection method and a technology for heterooxygen bacteria, which are applied in the field of microorganism detection, can solve problems such as low detection accuracy, and achieve the effects of improving work efficiency, reducing difficulty and improving accuracy.

Pending Publication Date: 2022-05-27
CHINA SHENHUA COAL TO LIQUID & CHEM CO LTD +1
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  • Abstract
  • Description
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Problems solved by technology

[0006] The purpose of the present invention is to provide a detection method for the content of heterooxic bacteria in order to solve the problem of low detection accuracy when detecting the content of heterooxygen bacteria

Method used

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  • Method for detecting content of heterotrophic bacteria
  • Method for detecting content of heterotrophic bacteria
  • Method for detecting content of heterotrophic bacteria

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preparation example Construction

[0020] In a preferred embodiment, the method for preparing the mixed solution includes: first dissolving TTC in sterile water to obtain an aqueous TTC solution with a TTC content of 0.5-2 wt%; dissolving PCA in pure water, adding hydroxide sodium to obtain a PCA aqueous solution with a pH value of 7.0-7.5 and a PCA content of 2.2-2.5 wt%; and then mixing the TTC aqueous solution with the PCA aqueous solution to obtain a mixed solution containing PCA and TTC.

[0021] In the present invention, the sterile water refers to the water obtained after the pure water is sterilized at 121°C and 0.2MPa for 15 minutes; the pure water refers to the conductivity ≤0.10us directly prepared by the pure water machine / cm of primary water. TTC is 2,3,5-triphenyltetrazolium chloride, analytically pure. PCA is a plate counting medium, containing 0.5wt% tryptone, 0.25wt% yeast extract, 0.1wt% glucose, 1.5wt% agar, 97.65wt% pure water, and pH 7±0.2.

[0022] In a further preferred embodiment, the...

Embodiment 1

[0049] (1) Dissolve TTC in sterile water first to obtain a TTC aqueous solution with a TTC content of 2.0 wt%; then dissolve PCA in pure water, stir at 95° C. until completely dissolved, and use medical absorbent cotton and absorbent gauze for thermal filtration , after filtering, adding sodium hydroxide to adjust the pH value to obtain a PCA aqueous solution with a pH value of 7.2-7.4 and a PCA content of 2.35wt%; then mixing the above-mentioned TTC aqueous solution with the above-mentioned PCA aqueous solution to obtain a mixed solution containing PCA and TTC; wherein , the content of PCA in the mixed solution is 2.35wt%, and the content of TTC is 0.002wt%;

[0050] Sterilize the above mixture at 121°C and 0.2MPa for 15min to obtain PCA-TTC medium, and observe the color of PCA-TTC medium;

[0051] (2) Mix the primary circulating water and the secondary circulating water in a volume ratio of 1:1 to obtain a 500 mL mixed water sample, first add 0.4 mg of sodium thiosulfate to ...

Embodiment 2

[0054] The method is the same as that in Example 1, except that the contents of PCA and TTC in step (1) and the incubation time are different. The specific contents and test results are shown in Table 1.

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Abstract

The invention relates to the technical field of microbiological detection, and discloses a method for detecting the content of heterotrophic bacteria, which comprises the following steps: (1) sterilizing a mixed solution containing PCA and TTC at 114-126 DEG C and 0.12-0.3 MPa for 10-30 minutes to obtain a PCA-TTC culture medium; wherein the content of PCA in the mixed solution is 2.2 wt%-2.5 wt%, the content of TTC in the mixed solution is 0.0005 wt%-0.02 wt%, and the mass ratio of PCA to TTC in the mixed solution is (800-2200): 1; and (2) inoculating a water sample to be detected to the PCA-TTC culture medium for culture, and then counting according to a counting method to obtain the content of heterotrophic bacteria. According to the method provided by the invention, the difficulty of TTC sterilization operation can be reduced, the color contrast between the culture medium and a bacterial colony is increased, the accuracy of a heterotrophic bacteria detection result and the detection rate of an unqualified water sample are improved, the culture time is shortened to 66 hours from the existing 72 hours, the working efficiency can be improved, and the method is suitable for industrial popularization.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a method for detecting the content of heterooxobacteria. Background technique [0002] Heterotrophic bacteria are bacteria that utilize organic matter in the environment for oxidative fermentation to obtain nutrients required by cells. In industrial circulating cooling water, the growth and reproduction of heterotrophic bacteria is the fastest and the number is the largest, which is an indicative and representative bacterial species in circulating cooling water. Such bacteria can produce dense mucus, adhere to fine suspended solids in industrial circulating cooling water and other filamentous bacteria, molds, algae, protozoa, etc., thereby forming slime, clogging pipes, corroding equipment, affecting heat exchange efficiency, and seriously. can cause parking. [0003] my country's industrial circulating cooling water treatment design code (GB / T 50050-2017) requi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/06C12R1/01
CPCC12Q1/045C12Q1/06
Inventor 娄红杰张冠楠刘威
Owner CHINA SHENHUA COAL TO LIQUID & CHEM CO LTD
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