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In-situ detection method for microalgae content in ship ballast water

A technology of ship ballast water and detection method, which is applied in the direction of measuring device, biological test, material inspection product, etc. Capacitance, reduce requirements, simplify the effect of detection steps

Active Publication Date: 2022-06-03
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all these existing detection methods based on chlorophyll content need to extract chlorophyll in microalgae first, and need to go through steps such as filtration, grinding, refrigeration, and centrifugation.
At the same time, organic solvents such as acetone (acid) and methanol need to be used in the chlorophyll extraction process, causing harm to the environment
At present, in the literature and patent reports, there is still a lack of a simple, fast and reliable detection method to obtain the biomass of microalgae in ballast water.

Method used

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  • In-situ detection method for microalgae content in ship ballast water
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  • In-situ detection method for microalgae content in ship ballast water

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Effect test

Embodiment 1

[0035] Step 1): using high temperature co-precipitation synthesis method, with sodium yttrium fluoride as the matrix, Yb and Er ions as the luminescent center, the upconversion luminescent nanoparticle NaYF with a size of 20 nm coated with an oleic acid ligand on the surface was prepared 4 : 50% Yb, 10% Er, dispersed in cyclohexane;

[0036] The specific preparation steps are: use a pipette to absorb 3ml of oleic acid, 7ml of octadecene and 1.6ml of Y(CH) respectively. 3 CO 2 ) 3 (0.2M), 2ml Yb(CH 3 CO 2 ) 3 (0.2M), 0.4ml Er(CH 3 CO 2 ) 3 (0.2M) aqueous solution into a 50ml two-necked flask; place the two-necked flask in a heating mantle and keep warm at 130°C for 60min; after the temperature of the liquid in the two-necked flask drops to room temperature, use a pipette to absorb 2ml NaOH (0.5M) and 4ml NH 4 F (0.4M) methanol solution was transferred to a 15ml centrifuge tube, capped tightly, shaken well, and quickly poured into a two-necked flask, kept at a constant ...

Embodiment 2

[0043] Step 1): Utilize high temperature co-precipitation synthesis method, take yttrium oxysulfide as matrix, Tm, Er ion as luminescent center, prepare surface is covered by oleic acid and oleylamine ligand, the size is 80nm up-conversion luminescent nanoparticles Y 2 O 2 S: 4% Tm, 40% Er dispersed in cyclohexane;

[0044] The specific preparation steps are as follows: suck 2.5ml oleic acid, 17ml oleylamine and 20ml octadecene with a pipette respectively, and weigh 0.28mol yttrium acetylacetonate, 0.2mol erbium acetylacetonate, 0.02mol thulium acetylacetonate and 0.5mol acetylacetonate Sodium was placed in a 50ml two-necked flask; the two-necked flask was placed in a heating mantle and kept at 120°C for 30min; after the temperature of the liquid in the two-necked flask dropped to room temperature, 0.5mol S powder was added, vacuumed at 110°C for 8min, and then passed through Nitrogen for 3min, repeated three times; adjust the temperature of the heating mantle so that the so...

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Abstract

The invention belongs to the technical field of ship ballast water microalgae content detection, and particularly relates to an in-situ detection method for the ship ballast water microalgae content. According to the method, the emission peak of a fluorescent probe is highly overlapped with the absorption peak of ballast water microalgae chlorophyll a to generate competitive emission to cause the change of the fluorescence intensity of the probe, and a relation model between the microalgae density and the luminous intensity of the probe is established, so that the biomass of microalgae in a ballast water sample is quickly calculated. Near-infrared light (NIR) is adopted as an excitation light source, so that the phototoxicity is low, the background fluorescence is low, and the detection sensitivity is high; the adopted inorganic upconversion nano particles are stable in light physical and chemical properties, free of light flickering and light bleaching phenomena and suitable for corrosive ballast water environments; the surface functional modification endows the probe with good water solubility and biocompatibility, the probe can directly enter microalgae cells, and in-situ rapid detection of the number of microalgae cells in ballast water is realized.

Description

technical field [0001] The invention belongs to the technical field of detection of microalgae content in ship ballast water, and in particular relates to the use of surface-functionalized red up-conversion nano-fluorescent probes to achieve in-situ rapid detection of microalgae content through a competitive fluorescence analysis method. Background technique [0002] With the rapid development of society, the economy tends to be globalized, the international economic and trade is increasingly prosperous, and ships carry more than 90% of the freight tasks. In order to improve the seaworthiness of the ship by adjusting the draft and stability of the ship, reducing the deformation of the hull and the resistance of the hull and reducing the vibration of the hull, the ship generally uses water as the ballast. However, the discharge of ship's ballast water has also become the main way for marine organisms and pathogens to spread, increasingly damaging the marine biological chain a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/64G01N21/01G01N33/569G01N33/58
CPCG01N21/359G01N21/6486G01N21/01G01N33/56961G01N33/582G01N2333/405
Inventor 田莹辛芳云付姚邢明铭庞强李佳瑶
Owner DALIAN MARITIME UNIVERSITY