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Phosphorus-solubilizing fungus, fungicide and application thereof

A fungus and phosphorus-dissolving technology, applied in the field of microorganisms, to achieve the effect of strong phosphorus-dissolving ability, broad application prospects and market value

Pending Publication Date: 2022-06-24
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, more and more attention has been paid to the isolation of phosphate-solubilizing bacteria, but these studies are mainly concentrated on dry land. Long-term flooding leads to a completely different ecological environment in paddy fields and dry land. Available phosphorus content for rice seedlings to grow and absorb

Method used

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  • Phosphorus-solubilizing fungus, fungicide and application thereof
  • Phosphorus-solubilizing fungus, fungicide and application thereof
  • Phosphorus-solubilizing fungus, fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening and identification of the phosphorus-dissolving fungus Penicillium ISA-C26 strain

[0026] 1. Strain screening

[0027] (1) Domestication and culture of phosphorus-dissolving fungi: Take soil samples of 0-5 cm on the surface of subtropical paddy fields in Changsha City, Hunan Province, place them in sterile bags for low-temperature storage, and bring them back to the laboratory for storage at 4°C. The topsoil of the paddy field was made into a soil suspension, and the concentration was diluted to 10 -6 Add 0.5 mL of insoluble calcium phosphate culture medium (NBRIY medium) to a sterile 96 deep-well plate, add 0.1 g / L bromocresol violet as an acid indicator to the culture medium, and inoculate 10 uL with a dilution concentration of 10 -6 times the soil suspension and placed in a constant temperature incubator (28°C) for 7-10 days. Then carry out the serial dilution again, take the prepared 10 -4 times and 10 -5 200 uL of 200 uL diluted bacterial ...

Embodiment 2

[0036] Example 2: Determination of phosphorus-dissolving activity of the phosphorus-dissolving fungus Penicillium ISA-C26

[0037] Phosphorus-dissolving ability of phosphate-solubilizing fungi in calcium phosphate solid medium, based on the phosphorus-solubilizing index of phosphate-solubilizing fungi on calcium phosphate solid petri dishes: three-point inoculation method Inoculate the screened fungi into calcium phosphate solid medium and place in a constant temperature incubator (28°C) for 5 days, and the camera took pictures to record the growth of phosphate-solubilizing fungi on the petri dish. figure 1 This is the topography of the phosphate-dissolving circle after the phosphate-dissolving fungus ISA-C26 was cultured in calcium phosphate solid medium for 5 days. Strong ability to dissolve phosphorus, and then use the image processing software Fiji to calculate the area of ​​the dissolved phosphorus circle S (cm 2 ) and strain area s (cm 2 ), calculate the phosphorus sol...

Embodiment 4

[0041] Example 4 Effect of Phosphorus Solubilizing Fungus ISA-C26 on Phosphorus Transformation in Paddy Soil

[0042] Soil sample collection and pretreatment: Soil samples were collected from the NPK (NPK) and NPK (NK) treatments in the long-term positioning rice experimental field in Jinjing, Hunan Province. The experimental field was in the drying stage. Three plots with different treatments were selected, and three topsoil cores (0-20 cm) were randomly taken from each plot with a soil drill, with a total of 9 soil cores, mixed uniformly, and used as a sample. The collected soil samples were manually removed from residual roots, and ddH was added. 2 O submerge, mix well, pass through a 2mm sieve, and let the soil settle at room temperature (about 2 to 3 days). Pour off the supernatant, divide each sample into two evenly, one is pre-incubated directly in a jar at 25°C, and the other is put into the same jar and autoclaved at 121°C for 30 min. Measure the respiration quotien...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a phosphorus-solubilizing fungus, a fungicide and application of the phosphorus-solubilizing fungus and the fungicide. The phosphorus-solubilizing fungus strain disclosed by the invention is named as Penicillium sp., the classification name is Penicillium sp., the phosphorus-solubilizing fungus strain is preserved in China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.23239, the preservation date is September 18, 2021, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The Penicillium sp. ISA-C26 has strong phosphate-solubilizing ability in both low-phosphorus environment and high-phosphorus environment, and the phosphate-solubilizing ability of the Penicillium sp. ISA-C26 is stronger than that of common phosphate-solubilizing fungi. The penicillium ISA-C26 is developed into a microbial inoculum, is applied to plant fertilizers, has important significance in effectively improving the content of rapidly available phosphorus in soil and the utilization rate of a phosphate fertilizer, and has wide application prospects and market values.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a phosphorus-dissolving fungus, an inoculum and an application thereof. Background technique [0002] The subtropical red soil area is the main producing area of ​​rice. Due to long-term weathering and leaching, the soil in this area is seriously deficient in phosphorus, which seriously affects the yield and quality of rice. At present, phosphate fertilizers mainly come from non-renewable phosphate rock. It is estimated that my country's phosphate rock will be exhausted within 50 years, which will put pressure on food security. The accumulation of total phosphorus in the soil exceeds 85 million tons of P 2 O 5 , indicating that the content of phosphorus in the soil is not low, but due to the strong adsorption of phosphorus by mineral particles, the soil phosphorus availability is poor, resulting in a low phosphorus utilization rate of only about 10%. Therefore, developi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/80
CPCC12N1/14C05F11/08Y02W30/40
Inventor 肖谋良魏晓梦祝贞科田宝虎葛体达陈剑平
Owner NINGBO UNIV
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