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Method for determining L-anserine in skipjack inulin tablet candy

A method of determination, the technology of anserine, applied in the field of detection, can solve the problems of high detection cost, achieve the effect of high durability and accuracy, and ensure quality

Pending Publication Date: 2022-06-24
BY HEALTH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the cost of improving the determination conditions and the determination equipment itself is relatively high, and the influence of the test sample on the accuracy and efficiency of the measurement is very important, so it is necessary to explore a new method for the determination of anserine, which should provide A method for preparing test samples that can be used to improve measurement accuracy and efficiency

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  • Method for determining L-anserine in skipjack inulin tablet candy
  • Method for determining L-anserine in skipjack inulin tablet candy
  • Method for determining L-anserine in skipjack inulin tablet candy

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Embodiment 1

[0032] (1) Preparation of test solution:

[0033] Take 10 samples, grind and mix evenly, accurately weigh 6 samples, each about 0.1g, put them in 50ml digestion tubes, add 5ml 6mol / L hydrochloric acid and 0.1g phenol, and shake evenly. Digest for 5h in an electric heating blast drying oven at 110°C. After taking it out and letting it cool, the solution was all transferred to an evaporating dish, and the solvent was evaporated to dryness in a 90°C water bath. After the evaporating dish is cooled, add an appropriate amount of water to dissolve the residue by ultrasonic, transfer the solution to a 100ml volumetric flask, make up to the mark with water, and shake well. Precisely measure 1ml of the above solution, put it in a 10ml volumetric flask, dilute to the mark with water, shake well, and filter through a filter membrane to obtain the test solution.

[0034] (2) The determination conditions of liquid chromatography and mass spectrometry are as follows:

[0035] Liquid chro...

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Abstract

The invention provides a determination method of L-anserine, the detection method mainly comprises optimization of mass spectrum conditions, selection of a chromatographic column, optimization of a mobile phase, and preparation of a standard solution and a test solution, the determination method is a liquid chromatography and mass spectrum combined method, and formic acid and acetonitrile are used as the mobile phase. According to the determination method, through optimization of a test sample preparation method and improvement of test conditions, compared with an existing determination method, the determination method is higher in precision, durability and accuracy, the content of the L-anserine in the skipjack inulin tablet candies can be rapidly and accurately obtained, and the quality of the candies is further guaranteed.

Description

technical field [0001] The invention belongs to the technical field of detection methods, and relates to a method for the determination of L-anserine in bonito inulin powder tablet candies. Background technique [0002] Anserine is a histidine dipeptide in animals, which is widely present in the skeletal muscle and brain of animals. Carnosine bioactive peptides have significant functions such as anti-oxidation, anti-aging, maintaining body pH stability and neuromodulation. In recent years, with the increasing demand of anserine in the field of special medical food and health food, it is more and more urgent to develop a fast and reliable detection method for anserine. [0003] At present, there is no relevant national standard for the detection of anserine, and the determination methods mainly include high performance liquid chromatography, ion exchange chromatography and capillary electrophoresis. However, these three methods have high requirements on the separation and pu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 黄成安刘光兰黄玲付小蓉蔡良平潘拾朝李丹
Owner BY HEALTH CO LTD