Lactococcus lactis subsp.lactis HFY14 and application thereof
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A technology of Lactococcus lactis and HFY14, applied in the field of microorganisms, to reduce the positive rate, improve renal function abnormalities, and regulate immune functions
Pending Publication Date: 2022-07-01
善恩康生物科技(苏州)有限公司
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Lactococcus lactis subspecies lactic acid is commonly used as a probiotic in the field of food technology, but little research has
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Embodiment 1
[0033] Example 1 Isolation and identification of bacterial strains
[0034] 1. Experimental materials
[0035] The traditional naturally fermented yak yogurt was collected from the herdsmen's homes in Hongyuan County, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan Province. After fully mixing the yogurt with a sterile spoon, 50 mL was drawn into a sterilized centrifuge tube with a sterile syringe and filled with low-temperature food. The sampling box was brought back to the laboratory and stored in an ultra-low temperature freezer at -80°C for future use.
[0036] 2. Experimental method
[0037] 1 Isolation and purification of lactic acid bacteria
[0038] Take 1 mL of yogurt samples respectively, and make 10-fold gradient dilution with sterile normal saline to 10 -6 CFU / mL, get bacterial liquid, then take 10 -4 CFU / mL, 10 -5 CFU / mL, 10 -6 100 μL of CFU / mL 3 gradients of bacterial solution were used for plate coating, cultured at 37°C for 24-48 h, and the colony mo...
Embodiment 2
[0061] Example 2 Animal Experiment Verification
[0062] 1. Materials and Reagents
[0063] Six-week-old SPF grade C57BL / J6 mice, half male and half male, totaling 50 mice, weighing 23 ± 2 g, were purchased from Chongqing Enswell Biotechnology Co., Ltd. The experiments in this study were approved by the Animal Experiment Ethics Committee of Chongqing Functional Food Collaborative Innovation with the approval number 2021050008B.
[0064] Mass fraction ≥ 98% pristane (Sigma Company, USA); mass fraction ≥ 97% prednisone (≥ 97%) (Beijing Bailingwei Technology Co., Ltd.); interleukin 2 (IL-2), IL-12, interferon γ (IFN-γ), immunoglobulin G (IgG), transforming growth factor-β (TGF-β) ELISA kit (Wuhan Saipei Biotechnology Co., Ltd.); serum creatinine (SCr), total cholesterol (TC), Urea nitrogen (BUN), albumin (ALB), triglyceride (TG) assay kit (Wuhan Saipei Biotechnology Co., Ltd.); TRIzol reagent, SYBR Green PCR Mastermix, SDS-PAGE gel preparation kit, BCA protein Concentration de...
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Abstract
The invention discloses a lactococcus lactis subsp. Lactis HFY14 and application thereof, and belongs to the technical field of biology, the lactococcus lactis subsp. Lactis is named as HFY14, the preservation number is CGMCC No.16647, the strain is separated from naturally fermented yoghurt, and the strain has the advantages that the strain can be used for preparing the lactococcus lactis subsp. Lactis HFY14 and the application of the lactococcus lactis subsp. Lactis HFY14; animal experiments, histopathologic observation and other experiments prove that the renal dysfunction of lupus nephritis mice induced by hypophytane through intragastric administration of viable bacterial liquid of the strain can be obviously improved, and the effect is close to that of a medicine prednisone. The invention provides a theoretical basis for the subsequent development and utilization of the lactococcus lactis subsp. Lactis HFY14 and the application of the lactococcus lactis subsp. Lactis HFY14 as a probiotic to improve the renal function of nephropathy, and the strain has the application potential of performing long-term intervention on lupus nephritis to improve the renal function.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactococcus lactis subsp. lactis HFY14 and its application in the preparation of medicines. Background technique [0002] The Qinghai-Tibet Plateau has a special geographical environment, with high altitude, low temperature and strong sunshine. These geographical factors have promoted the formation of large grasslands on the Qinghai-Tibet Plateau, which has been a natural area with developed animal husbandry since ancient times. Tibetan herdsmen living on the Qinghai-Tibet Plateau have a tradition of raising yak since ancient times. In addition to obtaining yak meat through animal husbandry, they also formed the habit of consuming yak milk and fermented yak milk to obtain yogurt. Modern scientific research has found that yak milk contains more nutrients than ordinary milk, and is rich in protein and trace elements. Yak yogurt obtained by natural fermentation of yak milk ...
Claims
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