Paralichthys olivaceus male related Dmrt1 recombinant protein and application thereof
A technology of recombinant protein and flounder, applied in the field of molecular biology, can solve the problems of not allowed to use, unsuitable for application, high cost of use, etc.
Pending Publication Date: 2022-07-15
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Abstract
The invention belongs to the technical field of molecular biology, and particularly relates to paralichthys olivaceus Dmrt1 recombinant protein and application. The paralichthys olivaceus Dmrt1 recombinant protein is obtained for the first time by utilizing an in-vitro recombinant expression technology, a preparation method of the recombinant protein is provided, and the paralichthys olivaceus gonad tissue is incubated by utilizing the recombinant protein, so that the paralichthys olivaceus Dmrt1 recombinant protein has biological activity. The recombinant Dmrt1 protein is injected into an abdominal cavity of a female adult paralichthys olivaceus, so that cell nucleus chromatin of an oocyte in an ovarian tissue of the female adult paralichthys olivaceus is condensed. Further injecting the vaccine into the abdominal cavity of the fry with undifferentiated gonad to respectively cause the obvious decrease and increase (plt) of the estrogen and androgen levels in the juvenile fish body; 0.05), and the male rate is increased from 0% to 82%. The recombinant protein can be used for sex control of fishes such as paralichthys olivaceus, and a new method and way can be provided for sex control and unisexual production in fish culture.
Application Domain
Microorganism based processesPisciculture and aquaria +2
Technology Topic
OocyteGonad tissue +14
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