Ticagrelor-related dyspnea genotype detection reagent or kit

Abstract

The invention relates to the technical field of medical biological detection, and provides novel application of a CYP3A5 gene mutation site rs776746, in particular to application in preparation of a diagnostic reagent or a diagnostic kit for detecting ticagrelor-related dyspnea genotypes. The invention further provides a biological preparation and a detection kit for carrying out ticagrelor related dyspnea genotype diagnosis by utilizing quantitative PCR (Polymerase Chain Reaction) detection aiming at the mutation site rs776746 of the CYP3A5 gene. The kit and the detection method disclosed by the invention are simple, convenient, reliable, short in period, high in specificity and easy to clinically popularize.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, and relates to the application of detecting the CYP3A5 gene mutation site rs776746 as a molecular marker in the diagnosis of ticagrelor-related dyspnea genotypes, in particular to the CYP3A5 gene mutation site rs776746 in the preparation of ticagrelor Application of related dyspnea genotype diagnostic reagents or kits. Background technique [0002] Ticagrelor is a new type of P2Y12 adenosine diphosphate (ADP) receptor antagonist. It is an active drug itself and does not require liver drug enzyme metabolism, and can directly and quickly inhibit platelet aggregation. [1] . The latest European and American guidelines recommend the use of ticagrelor antiplatelet therapy as a priority for ACS patients [2,3] . The PLATO study showed that although the composite incidence of cardiovascular death, myocardial infarction, and stroke was lower in the ticagrelor group than in the clopi...

AI Technical Summary

Problems solved by technology

Sanger sequencing method is a traditional method of detecting SNP. Sanger sequencing method starts from a fixed point of nucleotides, ends at a specific base at random, and performs fluorescent labeling after each base. , produce four groups of nucleotides of different lengths ending with A, T, C, G, and then detect by electrophoresis on urea-denatured PAGE gel, so as to obtain the visible DNA base sequence. This method has high accuracy. However, this method requires strong equipment support, and it is difficult for general laboratories to have this condition; correspondingly, the principle of fluorescent quantitative PCR method is based on DNA fragments with different GC content, and their Tm values ​​are different, so it can be judged according to the melting curve that contains SNPs The base composition type of the DNA fragment at the locus, compared with the Sanger method, fluorescent quantitative PCR only needs PCR to detect, and most laboratories have such conditions

[0006] At present, there is still a lack of a simple and accurate detection method for dyspnea caused by taking ticagrelor. If the SNPs related to dyspnea caused by ticagrelor can be found as biomarkers and the corresponding diagnostic kits can be developed, It will effectively promote the development of individualized antiplatelet therapy in my country, provide data support for clinicians to make medication decisions, and improve patient compliance and long-term prognosis

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