Lactobacillus rhamnosus strain LRa05 as well as application and product thereof

A technology of Lactobacillus rhamnosus and strains, which is applied in the field of Lactobacillus rhamnosus strain LRa05 and its uses and products, can solve problems that have not been reported, achieve the effect of reducing weight loss and great application prospects

Pending Publication Date: 2022-07-22
JIANGSU WECARE BIOTECHNOLOGY CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lactobacillus rhamnosus (Lactobacillus rhamnosus) is a kind of probiotic, although it has been reported that it has a relieving effect on diarrhea, but the effect of Lactobacillus rhamnosus on EPEC diarrhea with higher mortality is not known in the art Not yet reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus rhamnosus strain LRa05 as well as application and product thereof
  • Lactobacillus rhamnosus strain LRa05 as well as application and product thereof
  • Lactobacillus rhamnosus strain LRa05 as well as application and product thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0072] Experimental example 1. Isolation, screening and identification of Lactobacillus rhamnosus

[0073] (1) Isolation and screening of strains

[0074] Collect fecal samples from healthy human beings, take 1g to 9mL of sterile physiological saline, fully shake and disperse the samples, take 100μL of samples for gradient dilution, select an appropriate gradient and evenly spread them on MRS agar plates, place them under anaerobic conditions, and cultivate at 37 °C for 36 After ~48h, select suspected single colonies, carry out Gram staining, observe the morphological characteristics of the colonies by microscopy, and preliminarily screen the suspected Lactobacillus strains. The strain was repeatedly streaked and purified on MRS solid medium to obtain a purified strain of Lactobacillus.

[0075] MRS medium formula (g / L): peptone 10, beef extract 5, yeast powder 5, glucose 20, anhydrous sodium acetate 5, diammonium hydrogen citrate 2, Tween 80 1, K 2 HPO 4 2. MgSO 4 0.2, ...

experiment example 2

[0084] Experimental Example 2. Inhibitory effect of Lactobacillus rhamnosus LRa05 on EPEC

[0085] The Lactobacillus rhamnosus LRa05 stored in the glycerol tube was first activated on the MRS plate for 2-3 times, and then a single colony was picked and inoculated into the MRS liquid medium, and the seed liquid was obtained by anaerobic cultivation at 37 °C; The solution was inoculated into MRS liquid medium at a ratio of 2%, and cultured at 37° C. for 17-24 h to obtain a culture medium. Collect the centrifuged cells, resuspend them in sterile saline, and adjust the concentration to 10 8 -10 9 CFU / mL to get bacterial suspension. After collection and centrifugation, the supernatant was filtered through a 0.22 μm microporous membrane to obtain metabolites.

[0086] After the indicator strain EPEC was activated on TSA solid plate medium, a single colony was picked and inoculated into TSA liquid medium. After continuous transfer for two generations, centrifugation was performed to...

experiment example 3

[0090] Experimental example 3. Lactobacillus rhamnosus LRa05 reduces the adhesion rate of EPEC to colonic epithelial cells

[0091] HT-29 cells were cultured in DMEM complete medium (containing 10% fetal bovine serum, 1% penicillin and streptomycin solution) at 37°C containing 5% CO 2 In a constant temperature cell incubator, the cells were grown to a density of 80%, digested and passaged to a 24-well plate for about 10 5 cells / well, and after growing to a monolayer, replace with fresh medium without antibiotics.

[0092] Three experiments were used to study the adhesion inhibition effect of Lactobacillus rhamnosus LRa05 on EPEC. The EPEC used in the experiment was 10 7 CFU / well, 10 for Lactobacillus rhamnosus 8 CFU / well. The experiment was divided into four groups, each group did three parallel experiments, and repeated the experiment three times: (1) positive control group: EPEC was added to incubate for 3 hours; (2) competition group: EPEC and L. rhamnosus LRa05 were sim...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a lactobacillus rhamnosus strain LRa05 as well as application and a product of the lactobacillus rhamnosus strain LRa05, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus strain LRa05 is CGMCC (China General Microbiological Culture Collection Center) No.24377, and the lactobacillus rhamnosus strain LRa05 is a lactobacillus rhamnosus strain LRa05. The invention also provides application of the strain LRa05 in preparation of a product for relieving EPEC diarrhea and a product for relieving EPEC diarrhea based on the strain LRa05. The lactobacillus rhamnosus strain LRa05 can well inhibit EPEC in vitro, can greatly reduce the adhesion effect of EPEC on intestinal epithelial cells, and can greatly reduce the death rate, diarrhea score and weight loss of EPEC challenged mice.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a Lactobacillus rhamnosus strain LRa05 and its use and products. Background technique [0002] Diarrhea is a common gastrointestinal symptom, which refers to the frequency of defecation significantly exceeding the usual habit (more than 3 times / day), thin stool, increased water content, daily defecation volume exceeding 200g, or containing undigested food or pus and blood , A clinical state of mucus. Diarrhea is often accompanied by symptoms such as a sense of urgency to defecate, anal discomfort, and incontinence. Diarrhea can be divided into infectious diarrhea and non-infectious diarrhea according to the etiology. Infectious diarrhea is caused by pathogens. Common pathogens include bacteria, viruses, and parasites. Among them, EPEC diarrhea is a kind of infectious diarrhea. EPEC diarrhea refers to diarrhea caused by Enteropathogenic E. coli (EPEC). At pre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/12A61P31/04A23L33/135C12R1/225
CPCC12N1/20A61K35/747A61P1/12A61P31/04A23L33/135A23V2002/00A23V2400/175A23V2200/3204Y02A50/30
Inventor 方曙光朱明明左慧玉孔素芬刘欢陈明阳宋沙沙黄琴琴
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap