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Method for extracting dimerized furanone compounds from litsea coreana and application of dimerized furanone compounds

A technology for dimerizing furanones and compounds, which is applied in organic chemistry, drug combination, antineoplastic drugs, etc., and can solve problems such as no reports of dimerizing furanones

Pending Publication Date: 2022-07-29
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Now for the research on chemical components in eagle tea, compounds such as flavonoids, polyphenols, coumarins, lignans, volatile oils, polysaccharides have been reported, or a kind of furanone compound and its The method and application of extraction and separation from eagle tea, but there have been no related reports on dimeric furanone compounds

Method used

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  • Method for extracting dimerized furanone compounds from litsea coreana and application of dimerized furanone compounds
  • Method for extracting dimerized furanone compounds from litsea coreana and application of dimerized furanone compounds
  • Method for extracting dimerized furanone compounds from litsea coreana and application of dimerized furanone compounds

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Experimental program
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Effect test

Embodiment 1

[0040] The method for extracting and separating the above-mentioned dimeric furanones from eagle tea includes the following:

[0041] 1) Take 17.81kg of eagle tea leaves from the source of leopard skin camphor, after drying and pulverizing, reflux extraction with 90% methanol for 3 times, each extraction is 3~4h, and the extract is concentrated under reduced pressure to obtain 2020g of methanol extract;

[0042] 2) after the methanol extract in 1) was dispersed in water, extracted 3 times with petroleum ether, ethyl acetate and n-butanol successively, each extraction part was concentrated under reduced pressure to obtain the extract of each part, wherein 889g of ethyl acetate extract was obtained ;

[0043] 3) Separating the ethyl acetate part extract in 2) by silica gel medium pressure column chromatography, using petroleum ether-ethyl acetate in volume ratios of 9:1, 4:1, 3:2, 2:3, 1:4, 1:0 gradient elution, divided into 6 components, numbered Fr.1~Fr.6;

[0044] 4) The th...

Embodiment example 2

[0048] Example 2: MTT assay to detect the cytotoxic effect of the difuranones on HCT-116, SW1990 and OSC-19 cell lines:

[0049] HCT-116, SW1990 and OSC-19 cells in logarithmic growth phase were selected, and the cell concentration was adjusted to 3 × 10 with 10% fetal bovine serum medium. 4 Cells / mL, 100 μL per well, seeded into 96-well flat-bottom cell culture plates, placed at 37°C, 5% CO 2 After culturing in the incubator for 12 h, 100 μL of samples were added, and the concentrations of the samples were 1, 5, 20, 40, and 80 μM, respectively, and five replicate wells were set up. at 37°C, 5%, CO 2Continue to cultivate for 48h in the incubator. Then, 20 μL MTT solution (5 mg / mL) was added to each well to continue culturing for 3 h, and the supernatant was discarded. Finally, add 150 μL DMSO to each well, shake at low speed for 20 min to fully dissolve the crystals, detect the absorbance value at a wavelength of 490 nm, and calculate the cell viability, cell viability=abso...

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Abstract

The invention discloses a method for extracting and separating a dimerized furanone component with a new skeleton structure from litsea coreana in a separation and extraction technology in the chemical field, which comprises the following steps: reflux extraction, solvent extraction, medium-pressure silica gel column chromatography, MCI column chromatography and semi-preparative high performance liquid separation, and finally the dimerized furanone compound is obtained. An MTT activity test shows that the compound has a remarkable cytotoxic effect on human colon cancer cells HCT-116, human tongue squamous carcinoma cells OSC-19 and human pancreatic cancer cells SW1990, IC50 of the compound is 7.436 mu M, 10.88 mu M and 9.476 mu M respectively, and the compound can be used for preparing novel potential anti-cancer drugs.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction, in particular to a method for extracting dimerized furanones from eagle tea and its application. Background technique [0002] The young shoots and leaves of Litsea coreana Lévl.var.lanuginosa (Migo) Yang et P.H.Huang is a medicinal and edible herbal tea widely distributed in Guizhou, Yunnan, Sichuan and other places, as well as remote mountain village residents in Xishui, Wuchuan, Xifeng, Meitan and Kaiyang in Guizhou still retain the habit of drinking eagle tea. Eagle tea has the aroma of Lauraceae, which has the effect of improving eyesight, relieving summer heat, quenching thirst, digestion and bloating. Modern pharmacological studies have shown that eagle tea has antioxidant, hypoglycemic, lipid-lowering, anti-inflammatory, antibacterial, antiviral, antitumor and other pharmacological activities, and has great development and application prospects. [0003] Now for the re...

Claims

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Application Information

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IPC IPC(8): C07D493/20A61P35/00
CPCC07D493/20A61P35/00Y02A50/30
Inventor 肖世基方亦可孙成新董敏健张茂生王刚杨建文
Owner ZUNYI MEDICAL UNIVERSITY
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