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Preparation method of embedded biological enzyme preparation

A technology of biological enzymes and preparations, applied in the field of preparation of embedded biological enzyme preparations, can solve the problems of poor stability of enzyme preparations, achieve the effect of improving stability and reducing self-instability problems

Active Publication Date: 2022-07-29
南京永正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art, the present invention provides a preparation method for embedding biological enzyme preparations, which solves the problem of poor stability of the existing enzyme preparations, and uses electrostatic adsorption to form an embedding protective film of polyanionic polysaccharides, and at the same time, utilizes fatty acid Form a reversible esterification reaction with polyanionic polysaccharides, and form a secondary embedding on the surface of the enzyme powder, which greatly improves the stability of the enzyme powder

Method used

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  • Preparation method of embedded biological enzyme preparation

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Effect test

Embodiment 1

[0024] In the preparation method of the embedded biological enzyme preparation, the enzyme powder is wrapped with fatty acid and polyanionic polysaccharide as a coating layer, and the enzyme powder is coated by the way of wrapping to obtain the embedded enzyme preparation.

[0025] The enzyme powder uses protease.

[0026] The polyanionic polysaccharide uses xanthan gum.

[0027] The fatty acid adopts water-soluble fatty acid, and adopts butyric acid.

[0028]The enzyme powder is doped with nano-ammonium carbonate, and the preparation method of the nano-ammonium carbonate includes the following steps: a1, passing ammonia gas into diethyl ether to form a solution to obtain ammonia gas-diethyl ether liquid, and the ammonia gas The concentration in the ether is 100g / L; a2, the sodium hydroxide is dissolved in the glycerol and stirred evenly to form a sodium hydroxide solution, the concentration of the sodium hydroxide in the glycerol is 200g / L, and the stirring speed is 100r / mi...

Embodiment 2

[0035] In the preparation method of the embedded biological enzyme preparation, the enzyme powder is wrapped with fatty acid and polyanionic polysaccharide as a coating layer, and the enzyme powder is coated by the way of wrapping to obtain the embedded enzyme preparation.

[0036] The enzyme powder uses mannanase.

[0037] The polyanionic polysaccharide adopts a combination of gum arabic and gelatin, and the mass ratio of the gum arabic and gelatin is 2:1.

[0038] The fatty acid adopts water-soluble fatty acid, and adopts butyric acid.

[0039] Further, the enzyme powder is doped with nano-ammonium carbonate, and the preparation method of the nano-ammonium carbonate includes the following steps: a1, passing ammonia gas into ether to form dissolution, and obtaining ammonia gas-ether liquid, so The concentration of the ammonia in the ether is 400g / L; a2, the sodium hydroxide is dissolved in the glycerol and stirred to form a sodium hydroxide solution, and the concentration of...

Embodiment 3

[0046] In the preparation method of the embedded biological enzyme preparation, the enzyme powder is wrapped with fatty acid and polyanionic polysaccharide as a coating layer, and the enzyme powder is coated by the way of wrapping to obtain the embedded enzyme preparation.

[0047] The enzyme powder adopts amylase.

[0048] The polyanionic polysaccharide adopts a combination of gum arabic and gelatin, and the mass ratio of the gum arabic and gelatin is 2:1.

[0049] The fatty acid adopts water-soluble fatty acid, and adopts butyric acid.

[0050] Further, the enzyme powder is doped with nano-ammonium carbonate, and the preparation method of the nano-ammonium carbonate includes the following steps: a1, passing ammonia gas into ether to form dissolution, and obtaining ammonia gas-ether liquid, so The concentration of the ammonia in the ether is 300g / L; a2, the sodium hydroxide is dissolved in the glycerol and stirred to form a sodium hydroxide solution, and the concentration of...

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Abstract

The invention belongs to the technical field of enzyme preparations, and particularly relates to a preparation method of an embedded biological enzyme preparation, which comprises the following steps: wrapping enzyme powder by using fatty acid and polyanion polysaccharide as a wrapping layer, and wrapping the enzyme powder in a wrapping manner to obtain the embedded biological enzyme preparation. The problem that an existing enzyme preparation is poor in stability is solved, an embedding protective film of polyanion polysaccharide is formed in an electrostatic adsorption mode, meanwhile, a reversible esterification reaction is formed by fatty acid and polyanion polysaccharide, secondary embedding is formed on the surface of enzyme powder, and the stability of the enzyme powder is greatly improved; during washing, ammonium carbonate mixed with the enzyme powder and a negative charge system of the polyanion polysaccharide repel each other in like polarity, and an excellent moisture absorption performance is utilized to destroy a precipitated coating system, so that the polyanion polysaccharide is re-dissolved, and the enzyme powder achieves a release effect.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparations, in particular to a preparation method of an embedded biological enzyme preparation. Background technique [0002] The application of enzyme preparation is an important milestone in the development process of liquid detergent technology. With the development of detergent formulation technology, whether it is powder detergent or liquid detergent, enzyme preparation has gradually become an indispensable builder in the formulation. The washing product added with the enzyme preparation not only increases the removal function of organic stains, but also improves the disadvantage of poor comprehensive detergency, reduces the washing temperature, saves energy, and at the same time alleviates the environmental problems caused by phosphate eutrophication. [0003] But enzymes, as biological agents, are easily deactivated by temperature, pH, and chemicals. How to solve the stability of the enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N9/96C01C1/26B82Y40/00
CPCC12N11/14C12N11/10C12N9/96C01C1/26B82Y40/00
Inventor 王仁
Owner 南京永正生物科技有限公司
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