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Liquid chromatographic analysis method for detecting ziprasidone in blood

A technology for liquid chromatography analysis and ziprasidone, which is applied in the field of liquid chromatography analysis for detecting ziprasidone in blood, can solve the problems of large sample volume demand, long analysis time and high analysis cost, and achieves good sensitivity and Accuracy, simplification of preprocessing steps, the effect of shortening analysis time

Pending Publication Date: 2022-07-29
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, high-performance liquid chromatography-mass spectrometry has high analysis costs, high requirements on the site, and requires professionals to operate, so it is not suitable for widespread use in small and medium-sized hospitals.
High-performance liquid chromatography has the advantages of accurate quantification, good reproducibility, and low cost. However, most of the reported methods in the literature have problems such as complicated pretreatment operations, large sample volume requirements, and long analysis time, which are not yet suitable. For high-throughput sample detection

Method used

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  • Liquid chromatographic analysis method for detecting ziprasidone in blood
  • Liquid chromatographic analysis method for detecting ziprasidone in blood
  • Liquid chromatographic analysis method for detecting ziprasidone in blood

Examples

Experimental program
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Effect test

Embodiment 1

[0122] The present embodiment is used to illustrate the specific detection steps of the liquid chromatographic analysis method for detecting ziprasidone in blood:

[0123] 1. Calibration of standard solutions

[0124] 1.1 Preparation of standard working solution:

[0125] Accurately weigh 2 mg of ziprasidone standard, put it in a 2 mL cryovial, add 2 mL of anhydrous methanol, and obtain standard stock solution A;

[0126] Take an appropriate amount of standard stock solution A, dilute it with 50v / v% methanol aqueous solution, the concentration of ziprasidone in the obtained standard working solution is 6400ng / mL, and then continue to dilute with 50v / v% methanol aqueous solution to prepare the concentration ziprasidone solutions of 200ng / mL, 400ng / mL, 600ng / mL, 800ng / mL, 1600ng / mL, 3200ng / mL, and 6400ng / mL, respectively, and stored at -80°C;

[0127] 1.2 Obtain the standard curve:

[0128] Firstly, 10μL of standard working solution and 90μL of blank serum were respectively t...

Embodiment 2

[0157] This example is used to illustrate the linear relationship and the limit of quantification of the method of the embodiment of the present invention:

[0158] Add 10 μL of the ziprasidone standard working solution of each concentration prepared above, add 90 μL of blank serum and mix for 1 min, add 300 μL of acetonitrile, vortex and mix for 3 min at 2000 rpm, centrifuge at 14000 r / min for 10 min, and draw 350 μL of the supernatant was injected and analyzed, and the concentration of ziprasidone was in the range of 20ng / mL to 640ng / mL. According to the measurement conditions of this example, the concentration was measured from low to high, and the quantitative chromatographic peak area-concentration was plotted , obtain the standard curve; prepare a series of low-concentration standard solutions, add 10 μL of ziprasidone standard solutions of various concentrations prepared above, add 90 μL of blank serum or plasma respectively, and mix well, after pretreatment, injection a...

Embodiment 3

[0163] This example is used to illustrate the recovery rate and precision of the method of the embodiment of the present invention.

[0164] The standard working solution of ziprasidone was prepared into three concentrations of low, medium and high for sample addition recovery experiment and precision experiment, and the measurement was carried out according to the method of this example. The analysis and determination were repeated for 3 batches, and ziprasidone was recovered The rate and precision are shown in Table 2, respectively. The average recoveries of ziprasidone in the low, medium and high addition levels ranged from 99.0% to 101.3%, and the relative standard deviations were 0.83% to 1.96%. The results are shown in Table 2.

[0165] Table 2 Ziprasidone spiked recovery and precision

[0166] Scalar 40ng / mL 80ng / mL 320ng / mL The average recovery rate 100.5％ 101.3％ 99.0％ Precision RSD 6.76％ 1.20％ 1.40％

[0167] Based on the above ver...

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Abstract

The invention relates to the technical field of medicine detection, in particular to a liquid chromatographic analysis method for detecting ziprasidone in blood. Comprising the following steps: calibrating a standard solution to obtain a standard curvilinear equation; treating to-be-detected blood to obtain a to-be-detected sample, adding the to-be-detected sample into the online solid-phase extraction device, and extracting the to-be-detected sample to obtain an extract; adding the extract into a high performance liquid chromatography analyzer for analysis, and calculating through a standard curve equation to obtain the concentration of ziprasidone in the sample to be detected. The method provided by the invention can simplify the pretreatment steps, reduce the sample amount and shorten the analysis time, is suitable for large-flux sample detection, improves the detection efficiency, and has good sensitivity and accuracy.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a liquid chromatography analysis method for detecting ziprasidone in blood. Background technique [0002] Ziprasidone is an atypical antipsychotic drug belonging to the benzothiazole piperazine class of compounds. Its chemical structural formula is as follows: [0003] [0004] In vitro studies have shown that ziprasidone has an antagonistic effect on dopamine D2, 5HT2A, and 5HT1D receptors, and an agonistic effect on 5HT1A receptors. It has high affinity for receptors such as dopamine D2, D3, serotonin 5HT2A, 5HT2C, 5HT1A, 5HT1D, α1-adrenergic, and moderate affinity for histamine H1 receptors. No affinity was seen for other tested receptors / binding sites. Ziprasidone inhibits synaptic reuptake of serotonin and norepinephrine. Ziprasidone is mainly used for the treatment of schizophrenia, and it has a good effect on acute or chronic, initial or relapsing schizoph...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/60G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/6052G01N30/8627G01N30/74G01N2030/027G01N2030/062G01N2030/047Y02A50/30
Inventor 刘杏立贾永娟刘春冉倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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