Preparation method of regenerated silk fibroin/epsilon-polylysine composite hydrogel as well as product and application of regenerated silk fibroin/epsilon-polylysine composite hydrogel
A technology of regenerating silk fibroin and composite hydrogel, applied in bandages, photovoltaic power generation, medical science, etc., to achieve good mechanical properties
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Embodiment 1
[0036] Example 1. Preparation of RSF / ε-PLL composite hydrogel
[0037] The regenerated silk fibroin (RSF) and ε-polylysine (ε-PLL) were 15:0, 14:1, 13:2, 11:4, 7:8 according to the mass ratio, and RSF and ε were controlled -The total concentration of PLL is 75mg / mL, first add RSF solution and then add ε-PLL solution, add ddH 2 O make up the volume to 820 μL, turn the mixture on the rotary mixer to fully mix the system, and the conditions are 4 °C and 6 rpm for 10 min; then add 100 μL of 10×PBS solution to the system, and then continue to add 40 μL of 100 mg / mLLEDC solution and 40 μL of 100 mg / mL NHS solution; continue to invert on a rotary mixer to fully mix the system, and the conditions are 4°C and 6rpm for 5min.
[0038] At the same time, the system without the cross-linking agent EDC / NHS was set as the control, the difference was that the EDC and NHS solutions were added and replaced with an equal volume of ddH 2 O.
[0039] The composite hydrogel preparation system sa...
Embodiment 2
[0041] Example 2. Characterization of composite hydrogels
[0042] 1) Macroscopic morphology of RSF / ε-PLL composite hydrogel
[0043] The sample prepared in Example 1 was placed in a -20°C refrigerator for more than 12 hours. After taking it out, the lid of the centrifuge tube was quickly opened and placed in a freeze-drying machine that was cooled in advance for freeze-drying. The freeze-drying time was 48 hours. Carefully take out the dried sponge-like support, carefully cut a thin piece inside with a blade, be careful not to squeeze the cross-section to cause deformation; flatly adhere the thin piece of sample to the electron microscope sample stage containing conductive tape, and plated with gold for 90s; put the electron microscope After the sample stage is placed correctly, turn on the instrument for observation, select the appropriate multiple to take pictures and save the pictures.
[0044] The result is as figure 1 As shown, the results show that all control samples...
Embodiment 3
[0058] Example 3. Properties and functions of regenerated silk fibroin / ε-polylysine composite hydrogel
[0059] 1) Biodegradability of RSF / ε-PLL composite hydrogels
[0060] The hydrogel sample prepared in Example 1 was lyophilized and placed in PBS or an enzyme solution for in vitro degradation test, and the results were as follows: Image 6 shown. The results showed that all samples degraded the fastest in the first two days. After that, except for the samples in PBS, which remained basically stable, the rest of the samples soaked in the enzyme solution would still degrade slowly ( Image 6 middle A). The samples were degraded significantly faster in enzyme solution than in PBS, especially in collagenase and papain, indicating that the prepared RSF / ε-PLL composite hydrogel could be effectively degraded by various proteases. Image 6 The middle B is the microscopic morphology of the cross-section of the sample hydrolyzed in the collagenase solution for 10 days. The results...
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