Method for preparing phosphatidylserine without organic solvent

A technology of phosphatidylserine and organic solvents, which is applied in the field of phospholipids, can solve the problems of high cost, difficulty in recycling and separating organic solvents, residues of solvents and plasticizers in products, etc., and achieves the effect of simple process and high conversion rate

Pending Publication Date: 2022-08-05
WENGYUAN GUANGYE QINGYI FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult and costly to recover and separate organic solvent

Method used

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  • Method for preparing phosphatidylserine without organic solvent
  • Method for preparing phosphatidylserine without organic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare 540mL of enzyme-containing aqueous solution, the enzyme activity of phospholipase D in the enzyme solution is 5.0U / mL, and the pH is adjusted to 6.7;

[0023] Add 100g soybean lecithin raw material (phosphatidylcholine content is 57%) and 80g L-serine into the enzyme-containing aqueous solution, homogenize and stir evenly, and react at 42°C for 14 hours;

[0024] After the reaction was completed, 540 mL of water was added to the reaction solution, the serine was dissolved by stirring, and the aqueous phase was centrifuged to obtain 180 g of centrifugal precipitation;

[0025] Wash the centrifugal precipitation with 900 mL of pure water, separate the water phase by centrifugation, filter and concentrate the water phase of about 1980 mL of the two centrifugal separation membranes (reverse osmosis membrane) to 300 mL, and obtain the L-serine aqueous solution for the next batch of reactions. The second centrifugal precipitation is 180 g ;

[0026] The two centrifug...

Embodiment 2

[0028] Prepare 540mL of enzyme-containing aqueous solution, the enzyme activity of phospholipase D in the enzyme solution is 5.0U / mL, and the pH is adjusted to 6.5;

[0029] Add 100 g of soybean lecithin raw material (the content of phosphatidylcholine is 57%) and 80 g of L-serine into the enzyme-containing aqueous solution, homogenize and stir evenly, and react at 42° C. for 16 hours;

[0030] After the reaction was completed, 540 mL of water was added to the reaction solution, the serine was dissolved by stirring, and the aqueous phase was centrifuged to obtain 180 g of centrifugal precipitation;

[0031] The centrifugal precipitate was washed with 940 mL of pure water, the water phase was separated by centrifugation, and the 2020 mL of the water phase separated by the two centrifugal separations was concentrated to 300 mL by membrane filtration to obtain an aqueous L-serine solution for the next batch of reactions, and the second centrifugal precipitation was 180 g;

[0032...

Embodiment 3

[0034] Prepare 540mL of enzyme-containing aqueous solution, the enzyme activity of phospholipase D in the enzyme solution is 5.5U / mL, and the pH is adjusted to 6.5;

[0035] Add 100g soybean lecithin raw material (phosphatidylcholine content is 55%) and 80g L-serine into the enzyme-containing aqueous solution, homogenize and stir evenly, and react at 42° C. for 16 hours;

[0036] After the reaction was completed, 540 mL of water was added to the reaction solution, the serine was dissolved by stirring, and the aqueous phase was centrifuged to obtain 186 g of centrifugal precipitate;

[0037] The centrifugal precipitate was washed with 930 mL of pure water, the water phase was centrifuged, and the water phase separated by two centrifugations was concentrated to 300 mL by membrane filtration of about 2010 mL, to obtain an aqueous L-serine solution for the next batch of reactions, and the second centrifugal precipitation was 180 g;

[0038] Vacuum freeze-drying to obtain 90 g of a...

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Abstract

The invention discloses a method for preparing phosphatidylserine without an organic solvent, which comprises the following steps: adding an enzyme-containing aqueous solution into soya bean lecithin and L-serine serving as raw materials, homogenizing and mixing for reaction, adding water into the reaction solution after the reaction is ended to dissolve the L-serine, centrifugally separating to obtain a water phase and a precipitate, washing the precipitate with water, centrifugally separating to obtain a water phase and a precipitate, and drying to obtain the phosphatidylserine. Combining water phases, concentrating through membrane filtration to recover L-serine, combining precipitates, and freeze-drying in vacuum to obtain a phosphatidylserine product. According to the method, phosphatidylserine is synthesized from soybean lecithin through an enzyme synthesis method, no organic solvent is used, the process is simple, energy consumption is low, and industrial production is easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, in particular to a method for preparing phosphatidylserine without organic solvent. Background technique [0002] Phosphatidylserine (PS), also known as serine phospholipid, diacylglycerol phosphoserine, PS for short, is a kind of ubiquitous phospholipid, usually located in the inner layer of the cell membrane, and is one of the important components of the brain cell membrane. Brain function, improve brain cognition, repair brain damage function. PS synthesis methods include chemical synthesis method and enzymatic synthesis method. The chemical synthesis method disclosed in the patent document CN103351403 has many side reactions, complex products, and difficult separation; It has the advantages of high efficiency and simplicity, so it has become the mainstream of industrial production. Soybean lecithin is dissolved in an organic solvent, L-serine is dissolved in water, and then the two-ph...

Claims

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Application Information

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IPC IPC(8): C12P7/6481C12P13/06
CPCC12P7/6481C12P13/06Y02P20/10
Inventor 李令星李富坚车日辉张俊斌吴寅
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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