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Electrogenerated chemiluminescence sensor, preparation method and application of electrogenerated chemiluminescence sensor in glucose detection

An electrochemistry and sensor technology, applied in the field of electrochemiluminescence sensors and preparation, can solve the problems of high cost, cumbersome operation steps, and reduced sensitivity, and achieve the effects of signal stability, ECL signal enhancement, and good stability

Pending Publication Date: 2022-12-02
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult for GOD to exist stably on the electrode, so it is often necessary to introduce additional materials to assist the fixation of GOD on the electrode, which often reduces the sensitivity, and also makes the operation steps cumbersome and high in cost.

Method used

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  • Electrogenerated chemiluminescence sensor, preparation method and application of electrogenerated chemiluminescence sensor in glucose detection
  • Electrogenerated chemiluminescence sensor, preparation method and application of electrogenerated chemiluminescence sensor in glucose detection
  • Electrogenerated chemiluminescence sensor, preparation method and application of electrogenerated chemiluminescence sensor in glucose detection

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Embodiment approach

[0039] The third embodiment of the present invention provides a method for preparing an electrochemiluminescence sensor, including the method for preparing the above-mentioned electrochemiluminescence sensing platform, using the obtained electrochemiluminescence sensing platform as a working electrode, and working Potassium persulfate was added to the solution.

[0040] The fourth embodiment of the present invention provides an application of the above-mentioned electrochemiluminescence sensor in detecting glucose.

[0041] The fifth embodiment of the present invention provides a glucose detection kit, including an electrochemiluminescence sensing platform, potassium persulfate and a buffer solution, and the electrochemiluminescence sensing platform is obtained by the above-mentioned preparation method.

[0042] Specifically, the buffer solution is phosphate (PBS) buffer.

[0043] In order to enable those skilled in the art to understand the technical solution of the present ...

Embodiment 1

[0044] Example 1CPNs PFBT - Preparation of COOH / GCE

[0045] CPNs PFBT -Preparation of COOH: Prepare PFBT and PSMA mother solution (1mg / mL) with THF, sonicate each for 15min, take 1mL PFBT and 200μL PSMA, dilute it to 2mL with THF, sonicate for 20min, and mix 2mL immediately under ultrasound The solution was poured into 10mL ultrapure water, ultrasonicated again for 15min, and N 2 Through a 1000μL pipette tip, slowly pass it under the liquid surface of a small beaker until all THF is blown out, and then filter with a 0.22μm filter tip to obtain CPNs PFBT -COOH.

[0046] figure 1 For the CPNs prepared in Example 1 PFBT - Visual observation of COOH under sunlight and UV light. It is clear from the figure that the CPNs PFBT -COOH is a good yellow emitter.

[0047] figure 2 For the CPNs prepared in Example 1 PFBT The topography of -COOH under TEM shows that the prepared CPNs PFBT -COOH is a spherical structure with relatively uniform dispersion below 100 nm.

[0048] ...

Embodiment 2

[0049] Embodiment 2CPNs PFBT -Build of COOH / GCE

[0050] Before modifying the electrode, the GCE is pretreated, polished and cleaned. Firstly, three Al particles with different particle sizes of 1.0 μm, 0.3 μm and 0.05 μm were sequentially used on the suede. 2 o 3 The powder is used to scratch "8" on the surface of GCE and grind and polish, each particle size of Al 2 o 3 The powders were ground 100 times until the GCE surface was bright. The residual Al on the GCE was then rinsed with ultrapure water 2 o 3 powder, and then put it in a centrifuge tube filled with ultrapure water and ultrasonically clean it three times, each time for 3 min. After cleaning with ultrapure water, transfer them to 1:1 HNO 3 , Ultrasound in absolute ethanol for 3 min. After the pickling and alcohol washing were completed, ultrasonic cleaning was performed three times in a centrifuge tube filled with ultrapure water, each time for 3 minutes. After the above treatment, the GCE is like a mirro...

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Abstract

The invention belongs to the technical field of electrogenerated chemiluminescence sensing, and relates to an electrogenerated chemiluminescence sensor, a preparation method and application of the electrogenerated chemiluminescence sensor in glucose detection. The electrochemiluminescence sensor comprises an electrochemiluminescence sensing platform and potassium persulfate, and a preparation method of the electrochemiluminescence sensing platform comprises the following steps: carrying out a co-precipitation reaction on poly [(9, 9-dioctylfluorenyl-2, 7-diyl)-co-(1, 4-benzo-thiadiazole)] (PFBT) and a polystyrene-maleic anhydride copolymer (PSMA) to obtain carboxyl functionalized CPNsPFBT-COOH, and carrying out a reaction on the carboxyl functionalized CPNsPFBT-COOH and potassium persulfate to obtain the electrochemiluminescence sensing platform. And the surface of the electrode is modified by adopting CPNsPFBT-COOH, so that the electrode is obtained. The electrogenerated chemiluminescence sensor provided by the invention can simplify glucose detection steps, reduce cost and realize convenient detection of glucose.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence sensing, and relates to an electrochemiluminescence sensor and its preparation method and its application in detecting glucose. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or in any form to imply that the information constitutes the prior art already known to those skilled in the art. [0003] The inventors have found that the detection of glucose by electrochemiluminescence (ECL) sensing requires firstly modifying the luminescent material on the electrode, and then modifying the glucose oxidase (GOD) on the electrode to catalyze the oxidation of glucose. The interaction with the luminescent material or co-reactant affects the ECL signal intensity of the platform, thereby realizing the detecti...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/26G01N27/30G01N27/327
CPCG01N21/76G01N27/26G01N27/30G01N27/3271
Inventor 张献侯鹏冲刘强卢倩乔从德
Owner QILU UNIV OF TECH
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