New strains of drechslera monoceras and control compositions containing the same
A technology of Dess mold and composition, which is applied in the field of weed control composition, can solve the problems such as reduction, and achieve the effect of reducing environmental pollution and increasing herbicidal activity
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[0031] Embodiment 1: Isolation of Desseria monoceratum of the present invention
[0032] Naturally infected barnyardgrass is collected, and the pathogen is isolated from diseased tissues of the blades and sheaths of the leaves. Specifically, cut off a tissue part of 1 square centimeter with damage in the center, soak the cut part in 70% ethanol aqueous solution for 1-2 seconds, and then soak it in an aqueous solution of sodium hypochlorite with an effective chlorine concentration of about 2% for 10 seconds. minutes to sterilize the surface of the excised part. The sterilized diseased tissue portion was washed three times with sterile water, placed on an agar medium supplemented with antibiotics, and then incubated statically in an incubator. The top of the growing reticular mycelium was picked under a light microscope and transferred to nutrient medium for pure culture.
Example
[0033] Test Example 1: Examination of Selective Herbicidal Activity and Identification of Isolated Mold
[0034] (1) Test of selective herbicidal activity
[0035] Different species of barnyard weeds and rice crops were cultured in test tubes under aseptic conditions to prepare test materials. Specifically, seeds of E. crus-galli var. crus-galli and rice crops, namely Nipponbare Koshihikari and Sasanishiki, were sterilized in the same manner as described above. Seeds of these crops were sown in test tubes filled with sterile distilled water and allowed to grow.
[0036] On the other hand, at 25°C, the completely isolated mold was cultured on a plate containing potato-dextrose agar medium (Difco) for 1 week, and then the outer edge of the obtained colony was excised using a cork borer to obtain Mecerial dishes for inoculation.
[0037] The mecerial discs were inoculated into the test tubes in which barnyard weeds or rice crops had been grown, and then the plants were grown...
Example
[0044] Test Example 2: Differences between Desseria monoceratum strains according to the present invention and known bacterial strains
[0045] Desseria unicacerata MH-1901 and MH-2001 strains and known strains of Dessia unicacerata MH-9011 according to the present invention (according to the Chordapest Treaty for the International Recognition of Deposits of Microorganisms for Patent Processes, deposited as No. FERM BP-3416 deposited in the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology) with DNA amplified by arbitrary primers using the RAPD method (Arbitrarily Amplified Polymorphic DNA, Williams, J.G.K, etc. (1990) Polymorphic forms can be used as group markers, Nucleic Acid Research, Vol.18) The electrophoretic patterns of PCR products obtained were compared.
[0046] DNA was extracted and purified from MH-1901, MH-2001 and MH-9011 according to conventional methods. The purified DNA and a commercially available PCR reagen...
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