New strains of drechslera monoceras and control compositions containing the same

A technology of Dess mold and composition, which is applied in the field of weed control composition, can solve the problems such as reduction, and achieve the effect of reducing environmental pollution and increasing herbicidal activity

Inactive Publication Date: 2004-08-11
MITSUI CHEM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem that the present invention seeks to solve is to reduce the disadvantages of conventional weed control methods in which synt

Method used

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  • New strains of drechslera monoceras and control compositions containing the same
  • New strains of drechslera monoceras and control compositions containing the same

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Embodiment 1: Isolation of Desseria monoceratum of the present invention

[0032] Naturally infected barnyardgrass is collected, and the pathogen is isolated from diseased tissues of the blades and sheaths of the leaves. Specifically, cut off a tissue part of 1 square centimeter with damage in the center, soak the cut part in 70% ethanol aqueous solution for 1-2 seconds, and then soak it in an aqueous solution of sodium hypochlorite with an effective chlorine concentration of about 2% for 10 seconds. minutes to sterilize the surface of the excised part. The sterilized diseased tissue portion was washed three times with sterile water, placed on an agar medium supplemented with antibiotics, and then incubated statically in an incubator. The top of the growing reticular mycelium was picked under a light microscope and transferred to nutrient medium for pure culture.

Example

[0033] Test Example 1: Examination of Selective Herbicidal Activity and Identification of Isolated Mold

[0034] (1) Test of selective herbicidal activity

[0035] Different species of barnyard weeds and rice crops were cultured in test tubes under aseptic conditions to prepare test materials. Specifically, seeds of E. crus-galli var. crus-galli and rice crops, namely Nipponbare Koshihikari and Sasanishiki, were sterilized in the same manner as described above. Seeds of these crops were sown in test tubes filled with sterile distilled water and allowed to grow.

[0036] On the other hand, at 25°C, the completely isolated mold was cultured on a plate containing potato-dextrose agar medium (Difco) for 1 week, and then the outer edge of the obtained colony was excised using a cork borer to obtain Mecerial dishes for inoculation.

[0037] The mecerial discs were inoculated into the test tubes in which barnyard weeds or rice crops had been grown, and then the plants were grown...

Example

[0044] Test Example 2: Differences between Desseria monoceratum strains according to the present invention and known bacterial strains

[0045] Desseria unicacerata MH-1901 and MH-2001 strains and known strains of Dessia unicacerata MH-9011 according to the present invention (according to the Chordapest Treaty for the International Recognition of Deposits of Microorganisms for Patent Processes, deposited as No. FERM BP-3416 deposited in the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology) with DNA amplified by arbitrary primers using the RAPD method (Arbitrarily Amplified Polymorphic DNA, Williams, J.G.K, etc. (1990) Polymorphic forms can be used as group markers, Nucleic Acid Research, Vol.18) The electrophoretic patterns of PCR products obtained were compared.

[0046] DNA was extracted and purified from MH-1901, MH-2001 and MH-9011 according to conventional methods. The purified DNA and a commercially available PCR reagen...

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Abstract

Cells of a novel strain of Drechslera monoceras having practical weed controlling activity against barnyard grass (Echinochloa spp.) at temperatures ranging from 26 DEG C. to 35 DEG C., and exhibiting no pathogenicity to major crops, including rice, are used as a herbicidal component.

Description

technical field [0001] The present invention relates to a Drechslera monoceras strain having herbicide activity against barnyard barnyardgrass in the temperature range of 26°C to 35°C and a weed control composition comprising the same as an active ingredient. Background technique [0002] Environmental pollution caused by synthetic agricultural chemicals has become a modern environmental and social problem. Therefore, new herbicides utilizing natural microorganisms are being tested as a new type of agricultural chemicals that will not pollute the environment. For example, DeVine (active ingredient: Phytophthora palmivola) is available in the U.S. as a weed control composition against vine, romoaceae weeds, College (active ingredient: Colletotrichum Gloeosporioides), Biomal (active ingredient, malvae species in Pleurotus sp.) and Luboa II (active ingredient: cuscutae species within P. spinosa) as weed control agents for G. chinensis, a leguminous wee...

Claims

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Application Information

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IPC IPC(8): A01N63/30C12N1/14
CPCA01N63/04C12N1/14A01N63/30
Inventor 山口健一石原一人深井俊吾平濑寒月中村武史西田诚
Owner MITSUI CHEM INC
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