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Antisebum and antioxidant composion

A composition and anti-sebum technology, applied in skin care preparations, cosmetics, plant raw materials, etc., can solve the problem of no disclosed antioxidant activity, no disclosed preparation method, use and activity of alcohol fraction or low molecular weight fraction, no disclosure Disclosure of issues such as anti-sebum activity of guggal to reduce the appearance of wrinkles and aging skin, improve radiance, and improve oil control

Inactive Publication Date: 2000-03-29
UNILEVER NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above documents disclose neither the anti-sebum activity nor the antioxidant activity of guggal or guggal or its fractions
In addition, as far as the fraction of gugulipid is concerned, the above-mentioned documents only disclose the preparation method and use of the fraction rich in gugusterone (ethyl acetate), but do not disclose the preparation method of the alcohol fraction or the low molecular weight fraction , use and activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064]This example illustrates the procedure for the fractionation of gugulipid into fractions of increased polarity (procedure A) and for the preparation of a low molecular weight fraction of gugulipid (procedure B). Procedure A Materials: Gugulipid (Lot No. 42941, Lipo Chemicals Inc. Paterson NJ) medium pressure column (5 cm internal diameter X 62 cm long) silica (Gel, Merck), Aldrich, Cat no. 22, 719-6, Grade9385, 230, 400, mesh 60A. Thin Layer Chromatography Plate, LHP-kk 20 x 10cc Lot # 004966, Cat # 4805-711 Whatman. Hexane, HPLC Grade (Fisher) Ethyl Ethanol, HPLC Grade (Fisher) Methanol, HPLC Grade (Fisher) Chloroform, HPLC Grade (Fisher) Phosphoric Acid (Fisher) Copper Sulfate CuSO 4 (Fisher) petroleum ether (Fisher) 24 beakers (400ml) scintillation tube capillary (5μl) 2 measuring cylinders (2000ml) 1 developing dish 1 oven (Napco type 420) 1 heating / stirring plate 2 round bottom flasks ( 500ml) rotary evaporator water (Milli-Q water) method:

[0065] 1. Weigh 5.06...

Embodiment 2

[0112] This example reports the in vitro analysis of the sebum suppressing effect of gugulipid and its various fractions.

[0113] In vitro test of fat formation by sebocytes

[0114] Human sebaceous glands were isolated from the nose of a male (60 years old) and cultured using the soak tissue culture technique (Bajor et al, J. Invest Dermatol. 102:1994, p. 564). These sebocytes aggregate the intracellular lipid droplets characteristic of mature human sebum.

[0115] Add the harvested and passaged sebocytes to each well of a 48-well tissue culture dish and incubate in 7.5% CO 2 and cultured at 37°C for 10 days. On the day of the experiment, the growth medium was removed and the sebocytes were washed three times with phosphate-buffered saline (PBS). 0.5 ml of fresh PBS was added to each well at various concentrations shown in Table 1, and 10 μl of the reagent to be tested was added. Three wells use the same sample. Control samples consisted of phosphate-buffe...

Embodiment 4

[0129] This example reports chemical tests and in vitro analyzes of the antioxidant activity of gugulipid and its various fractions. Chemical test:

[0130] The chemical assay measures the antioxidant activity of the various test compounds indicated in Table 3 (all tested at 0.08% except for the low molecular weight fractions which were tested at 1.67% and 0.17%). Solubilize 2,2'-azino-bis-(3-ethylbenzothiazoline sulfonate) (6.1 μmol / l) and intermyoglobin ( metmyoglobin) (610 μmol / l). The test substances are then added and the absorbance at 734 nm is measured before and after addition of the substrate, hydrogen peroxide (250 μmol / l). The initial absorbance was subtracted from the absorbance containing the matrix. This prevents absorbance differences caused by the test compound itself. Absorbance changes over time, so multiple times were examined for absorbance. Results are expressed as percent oxidation relative to a control sample (100% oxidation) containing all test com...

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PUM

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Abstract

Gugulipid (a lipophilic ethyl acetate extract from C. mukul or C. wightii) and an alcoholic fraction of gugulipid as antisebum and / or antioxidant active in cosmetic skin care compositions and methods is disclosed. Gugulipid from C. mukul and its alcoholic fraction are capable of delivering dual benefit to the skin: controlling or preventing sebum secretion (oily skin conditions) and protecting the skin from free radical damage.

Description

field of invention [0001] The present invention relates to methods and compositions for controlling or preventing sebum secretion by sebocytes, controlling or preventing oily skin conditions and protecting the skin from free radical activity. Background of the invention [0002] Sebum is skin oil produced by sebocytes (the cells of the sebaceous glands in the skin), which are then secreted onto the skin's surface. Excess sebum on the surface of the skin results in a condition known as "oily skin". Oily skin has a shiny, undesired appearance and an unpleasant feel. There are many existing methods and compositions which have attempted to control excess sebum secretion, but none of them provide fully satisfactory results. [0003] The formation of free radicals in the skin does not appear to be related to sebum production. Low levels of free radicals are formed in the skin as part of natural metabolic pathways. Increased levels of free radicals are affected by UV exposure a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/00A61K8/92A61K8/96A61K8/97A61K36/328A61Q5/00A61Q19/00
CPCA61K2800/522A61Q19/00A61K8/922A61Q19/008
Inventor J·P·麦库克J·M·科雷伊P·L·多罗吉J·S·巴约尔H·E·克纳格斯B·A·朗格E·夏佩M·塔尔曼
Owner UNILEVER NV
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