Attenuated live vaccine of vibrion from eel

Abstract

The invention provides an attenuated live vaccine of Vibrio anguillarum. Compared with the wild strain MVM425, the vaccine has obvious low toxicity and immune protection rate, and does not contain any antibiotic resistance markers. Tests have proved that the live attenuated vaccine can effectively protect susceptible fish from the infection of the wild strain of vibrio anguillarum with strong vibriosis pathogenicity.

Description

technical field

[0001] The invention relates to an attenuated live vaccine for aquaculture fish, in particular to an attenuated live vaccine of Vibiro anguillarum. Background technique

[0002] With the gradual development of the marine aquaculture industry, a large-scale outbreak of various diseases followed, and the disease problem became increasingly prominent, restricting the further development of the marine aquaculture industry. The prevention and control of diseases has become the primary problem that mariculture should solve.

[0003] Vibrio eelium is an important bacterial pathogen that seriously harms aquaculture fish in my country and the world. It mainly causes vibriosis such as hemorrhagic septicemia in farmed fish. The main virulence factor contained in Vibrio eelium is An iron uptake system encoded by a pJM1-like plasmid (Crosa, J.H., Walsh, C.T. Genetics and assembly line enzymology of siderophore biosynthesis in bacteria. Microbiol ...