Aqueous extract of leonurus heterophyllus and its application in preparing pharmaceutical composition
A technology of motherwort and water extract, applied in Chinese herbal medicine motherwort water extract and its application field in preparing pharmaceutical composition
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Embodiment 1
[0023] Motherwort water decoction is filtered and concentrated, and then checked by high-performance liquid chromatography-mass spectrometry, using photodiode array detector (DAD) and electrospray (ESI) mass spectrometry, which contains stachydrine (Stachydrine), mountain Naphthalene (Kaempferol) and quercetin (quercetin) and other main components.
Embodiment 2
[0025] Take motherwort water extract, add appropriate amount of microcrystalline cellulose, starch or other excipients, and make capsules, tablets, powders, granules, pills and other oral dosage forms according to conventional methods.
Embodiment 3
[0027] The in vitro antioxidant effect of the motherwort water extract of the present invention was tested.
[0028] ①Observation of hypochlorous acid (HOCl) and peroxynitroso (ONOO) in water extract of motherwort by using pyrogallol red bleaching experiment - ) scavenging effect, the results showed. ② use ABTS + (2,2'-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid)) free radical scavenging experiment to calculate TEAC (Trolox-equivalent antioxidant capacity) value. The average TEAC values of the aqueous extract of Motherwort were 562% and 588% (with antioxidants ascorbic acid and Trolox as standards, respectively). ③Inhibition of ascorbic acid-ferric ion-induced lipid peroxidation experiment, the results confirmed that the motherwort water extract has a strong effect of scavenging hypochlorous acid, compared with the control antioxidant ascorbic acid, p<0.05.
[0029] The water extract of motherwort has the effect of scavenging peroxynitroso, and there is no statistica...
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