Prepn of microbial colony sludge for efficient treatment of sewage
A technology of microbial flora and sludge, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of governance failure, large equipment investment, and insignificant effect, and achieve the effect of stable structure and wide range of functions.
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Embodiment 1
[0021] Embodiment 1: Collection of engineered strains: The engineered strains of the present invention are collected from different sewage or sludge, preferably collected in an environment requiring treatment. The collection of sludge is to collect 50-100 grams of samples from the sludge and place them in sterile containers; the collection of water samples is to collect water samples from the hydrostatic layer in wide-mouth sterile bottles, and all samples should be tested within 24 hours, or 4 ℃ sealed storage.
Embodiment 2
[0022] Embodiment two, engineering original strain cultivation: according to the basic principle of microbial growth, the nitrogen source content of microbial culture medium is 1-10%, carbon source is 2-20%, inorganic ion 0.05-1%, organic matter 2-10% , growth-promoting factors such as vitamins and trace elements are 0.01-0.5%, and the balance is distilled water.
Embodiment 3
[0023] Embodiment three: photosynthetic bacteria separation and enrichment culture method: (1) photosynthetic bacteria culture medium formula is (percentage by weight): ammonium chloride 0.05-0.2, sodium acetate 0.1-0.2, magnesium chloride 0.01-0.02, calcium chloride 0.01- 0.02, calcium dihydrogen phosphate 0.02-0.05, dipotassium hydrogen phosphate 0.02-0.05, yeast extract 0.02-0.05, distilled water 99.0-99.5. (2) The separation and enrichment method is as follows: adjust the pH value of the culture medium to 7.0, and then sterilize at 115°C for 20 minutes. After cooling, add 3-5% of sewage or sludge to be separated, and heat Cultivate at 30°C under anaerobic light for 5-7 days, the culture solution changes from colorless to red, and then transfer culture for 3 times to obtain dark red liquid as photosynthetic bacteria liquid.
[0024] In the above examples, the preferred formulation of the photosynthetic bacteria culture medium is (percentage by weight): 0.1 ammonium chloride...
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