Special lactobacillus inoculum for silage and its making method

A technology of lactic acid bacteria and inoculants, applied in the preservation method, bacteria, and application of animal feed raw materials, can solve the problems of high loss rate, high commodity cost, and high cost, and achieve the effect of improving quality

Inactive Publication Date: 2003-04-16
王建华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most widely used lactic acid bacteria abroad include Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus xylosus, Pediococcus pentosaceus, Pediococcus acidilactici, Propionibacterium shermanii, etc. The carbon source of the main components of the fermentation medium is generally glucose, lactose, soluble starch and sucrose, and the nitrogen source is generally whey powder, yeast culture and hydrolyzed animal protein. The fermentation products of batch fermentation are generally separated by filtration, ultrafiltration or centrifugation. Concentration; the isolated high-purity bacteria are either spray-dried (high loss rate) or freeze-dried (high cost), the latter requires a corresponding freeze-drying protective agent, except that the freeze-drying equipment mainly comes from a one-time investment The protectiv

Method used

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  • Special lactobacillus inoculum for silage and its making method
  • Special lactobacillus inoculum for silage and its making method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0054] Low temperature storage is beneficial to prolong the shelf life. In particular, the preservation effect is best at -20°C, followed by 4°C and room temperature; at -20°C and 4°C, the number of viable bacteria during the 90-day storage period of PP bacteria does not decrease at all; the inactivation of viable bacteria at room temperature only 16% inactivated. Under the same conditions, the preservation effect of LP bacteria is obviously much worse. The inactivation rate is 78% at room temperature for 110 days, 0% at -20°C, and <5% at 4°C. Therefore, cryopreservation and vacuum packaging are necessary to prolong the shelf life of this product and ensure product quality. Embodiment 2: product Lactobacillus plantarum of the present invention neutralizes Pediococcus pentosaceae count

[0055] 1. Medium

[0056] (1) 1% sterile peptone buffer solution: Accurately weigh 5 g of peptone and dissolve in 500 ml of deionized water, dispense into conical flasks, place in a steriliz...

Embodiment 3

[0069] Embodiment 3, the influence of lactic acid bacteria inoculant of the present invention on corn silage quality and dairy cow production performance 1. Silage inoculant of the present invention

[0070] The amount of silage used per ton is 10 grams, and the dry powdered inoculant is completely dissolved in water according to the ratio of 5 grams per liter, and then the SIB-HA inoculant is sprayed into the silo with a motorized knapsack sprayer. On the crushed silage layer, after each layer of silage is laid, the inoculant solution is sprayed once, compacted back and forth with a crawler tractor, and other links are treated according to conventional silage measures, which will be used for the feeding test of dairy cows in the next year. 2. Silage quality assessment

[0071] Fresh whole-plant corn (spring corn variety Zhongyu No. 4) was chopped with a cutting machine at a dairy farm in the northern suburbs of Beijing, and the length was less than 50 mm. SIB-HA was inoculate...

Embodiment 4

[0115] On the other hand, the acetic acid levels in the silage of the treatment groups were lower than those of the control group (except on days 5 and 15), with a difference ranging from 5-8% to 29.8% (day 25). Clearly, this is the result of inhibited heterolactic fermentation. With the extension of silage time, the pH value of silage decreased. Moreover, the pH value of the treatment group silage corn was lower than that of the control group at most of the observed time points. The pH in corn silage has a close relationship with the content of lactic acid, but has little relationship with the content of volatile fatty acids. This is consistent with the difference in acidifying ability of these two types of organic acids. Example 4 Test of Dairy Cow Production Performance

[0116] During the first 30 days of the experiment, the silage of the SIB-HA treatment group and the control silage had no effect on the milk production of dairy cows. After 30 days, the effects of trea...

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Abstract

An inoculating lactic ferment for silage is prepared from two kinds of dried living bacteria powder, cellulase, semi-cellulase, carbohydrate, manganese sulfate and diatomite through medium-temp. pressure-reduction drying and gelatin-starch dual-coating or freeze-drying. Its advantages are great number of living bacteria 90 billions CFU/g, long storage period, and high effect (increasing milk by 283 kg).

Description

Technical field: [0001] The invention belongs to the field of feed processing, in particular to a special lactic acid bacteria inoculant for silage and a manufacturing method thereof. technical background: [0002] The dairy cattle industry in my country has developed rapidly. The output has increased by 5 times in the past 5 years. By 2000, it will reach 4.89 million heads (including adult fine breed cattle, improved cattle, young cattle and dual-purpose cattle for milk and meat production). The total milk output is 8.27 million tons. The average annual milk consumption per person is actually nearly 7 kilograms. If the average daily milk consumption per person is only 0.25 kilograms, the average annual milk consumption per person is nearly 81 kilograms. There is still room for development of more than 10 times the current basic level. It can be predicted that my country's dairy cows will develop in It will maintain a strong momentum for quite a long time, but what is not comp...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/163A23K20/189A23K20/20A23K30/15A23K30/18A23K50/10C12N1/20
Inventor 王建华时建忠刁其玉张帆刘立恒滕达吴子林李桂杰
Owner 王建华
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