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Biologic material for medical use and its preparing process and usage

A technology of biomedical materials and raw materials, applied in the field of biomedical materials, can solve the problems of fast degradation, yellowing and unsuitability of target materials, and achieve strong resistance to enzymatic degradation, good blood compatibility, and good air permeability Effect

Inactive Publication Date: 2004-11-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is to use natural biomaterials, such as collagen, chitosan, sodium diatomate and other modified biomaterials as wound dressings, hemostatic sponges (fibers), etc. Although these materials have clinical applications, they are due to the shortage of raw materials. , the extraction rate of collagen is low, the price of the target product is high, and the degradation rate is too fast, so it is not suitable for tissue engineering scaffold materials, and there is no large-scale production
However, the amount of glutaraldehyde is difficult to control. When it is too much, it will easily cause the target material to turn yellow and produce toxicity, which is not conducive to the culture and growth of tissue cells; when the amount is small, it will not achieve the purpose of improving collagen performance defects.
(4) Some experts pointed out that although the artificial skin has been applied clinically, it still does not have the function of a complete skin
These methods still do not address the shortage of raw material sources, expensive and insufficient crosslinking with glutaraldehyde, and the negative impact of synthetic materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of raw materials

[0045] (1) Preparation of skin collagen or skin gelatin:

[0046] Take 100g of pig skin and put it into a container, wash it with deionized water, add 2 times of deionized water, heat to 40°C, add 2.0g of soda ash, 2g of edible oil cleaning agent, 0.4g of 2709 protease, and 0.2g of lipase , keep warm and stir for 90 minutes, remove hair, skin and grease, etc., and then wash with deionized water; grind the above-mentioned treated skin, or mash it into a slurry, take 100g of the skin pulp, add 1 times the deionized water, and heat Heat to 80°C, keep warm for 90 minutes, cool down to 40°C, adjust the pH value to 6.5-7.0, add 0.3g of trypsin, keep warm and stir for 3 hours, heat to 75°C, keep warm for 60 minutes, cool down to 45-50°C, and centrifugally filter , concentrating the supernatant under reduced pressure to 8-10% effective matter content, molecular weight 9.0×10 4 ~1.8×10 5 , stored at low temperature for later use;

[0047] (2...

Embodiment 2

[0056] 1. Preparation of raw materials:

[0057] (1) Preparation of skin collagen or skin gelatin

[0058] Take 100g of calfskin and put it into a container, wash it with deionized water, add 1 times of deionized water, raise the temperature to 45°C, add 0.5g of soda ash, 2g of edible oil cleaning agent, 0.2g of AS1.398 protease, and 0.4g of lipase , heat preservation and stirring for 60 minutes, remove hair, skin and grease, etc., and then wash with deionized water; twist or mash the above-mentioned treated skin, take 100g of skin pulp, add 2 times deionized water, and heat up to 90 ℃, keep warm for 60 minutes, cool down to 45°C, adjust the pH value to 6.5-7.0, add 0.1g of trypsin, keep stirring for 9 hours, heat to 60°C, keep warm for 120 minutes, cool down to 45-50°C, centrifugally filter, and The supernatant liquid is concentrated under reduced pressure to an effective content of 8-10%, and a molecular weight of 1.6-2.5×10 5 , stored at low temperature for later use;

...

Embodiment 3

[0065] 1. Preparation of raw materials:

[0066] (1) Preparation of skin collagen or skin gelatin

[0067] Take 100g of calfskin and put it into a container, wash it with deionized water, add 1 times of deionized water, heat to 43°C, add 1.5g of soda ash, 2g of edible oil cleaning agent, 0.2g of 2709 protease, 0.3g of lipase, Insulate and stir for 70 minutes, remove hair, skin and grease, etc., and then wash with deionized water; grind or mash the above-mentioned treated skin, take 100g of skin pulp, add 1 times deionized water, and heat up to 85°C , keep warm for 75 minutes, lower the temperature to 43°C, adjust the pH value to 6.5-7.0, add 0.2g of trypsin, keep warm and stir for 6 hours, heat to 70°C, keep warm for 90 minutes, cool down to 45-50°C, centrifuge and filter the clear The liquid is concentrated under reduced pressure to an effective substance content of 8-10%, and a molecular weight of 6.0×10 4 ~1.4×10 5 , stored at low temperature for later use;

[0068] (2)...

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PUM

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Abstract

A biological material for medical purpose, and method for preparation and use, the preparing method includes taking collagen or gelatin 30-70 parts, gluglucosan 20-40 parts, chondroitin sulfate 0-40 parts and chitosan 0-40 parts. The preparing process comprises the steps of stirring homogeneously, decompressing, de-aerating, drying, steeping, deionized water washing, drying, split charging, packaging, and radiation sterilization treatment.

Description

1. Technical field [0001] The invention relates to a biomedical material, a preparation method and application thereof, and belongs to the field of biomedical materials. 2. Background technology [0002] Medical biomaterials play a very important role in clinical treatment and medical scientific research, especially in the research and application of tissue engineering. It is the key to the industrialization of tissue engineering. There are two main categories of medical biomaterials that have been developed so far. One is chemically synthesized, mainly polylactic acid (PLA) and polyglycolic acid (PGA). Many problems have not been solved, and its fatal flaw is that it can cause sterile inflammation of the tissue surrounding the material. Bi Hong and others solved this problem by synthesizing a new material, a copolymer of α-hydroxy acid and α-amino acid, "new tissue engineering scaffold material", "Journal of Biology", 2001, 18 (1). The second is to use natural biomaterial...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L27/50A61L27/60
Inventor 王坤余王碧
Owner SICHUAN UNIV
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