Preparationj process of high pureness phosphatidyl inositol

A phosphatidylinositol, high-purity technology is applied in the field of preparation of high-purity phosphatidylinositol, and can solve the problems of high solvent consumption, limited industrial-scale production, and low PI purity.

Inactive Publication Date: 2005-01-05
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the existing extraction method, the purity of PI prepared is not high, and often contains phosphatidylethanolamine (PE), phosphatidic acid (PA)
The combination of solvent extraction and column chromatography can improve the purity of PI, but the solvent consumption is large, the product is very little, the yield is very low, and the industrial scale production is limited. Because of this, the final product is very expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: 10g powdery soybean lecithin (PI content is 18%), joins in 40 ℃, in the alkaline ethanol (dehydrated alcohol: ammoniacal liquor=100:3, V / V) extract of five times volume, Stir and extract for one hour, centrifuge the extract, mix the residue with alkaline ethanol, repeat the same operation twice, and dry the insoluble matter in vacuum at 60°C to obtain 4.28g of powdered crude phosphatidylinositol, which contains phosphatidylinositol Inositol 41.5%, phosphatidic acid 39.4%. Weigh 1 g of the crude phosphatidylinositol, dissolve it in 60 ml of n-hexane, fully dissolve it and put it into a separatory funnel with 100 ml of 55% ethanol containing 0.325 g of anhydrous sodium acetate, shake it at room temperature for 1 minute, and let it stand overnight. The aqueous ethanol layer was separated, 0.03 g of calcium chloride was added, stirred for 15 minutes, and left to stand overnight at 4°C. The precipitate was separated by centrifugation and vacuum-dried to obtai...

Embodiment 2

[0018] Example 2: 20 g of DEAE-cellulose was weighed, suspended in 50 ml of 1N sodium acetate (pH 7.0), and allowed to stand overnight. After filtration, add water and stir slowly, filter, remove the filtrate, add methanol to the cellulose, stir slowly, filter and further wash with chloroform-methanol (1:4, V / V) to complete the activation of cellulose. Repeat the operation in Example 1 to obtain a high-purity phosphatidylinositol sample, weigh 1g and add it to 100ml of chloroform-methanol (1:4, V / V) mixed solution containing activated cellulose, and stir at room temperature for 1 hour , filtered, washed with 200ml of chloroform-methanol (1:4, V / V) mixture, then eluted with 400ml of chloroform (1:4, V / V) containing 0.1% sodium acetate (W / V), and collected The eluate was concentrated in vacuo at 30°C, and 10 times the volume of acetone was added, the separated precipitate was dissolved in a small amount of chloroform, and five times the volume of 30% alcohol was added to soak fo...

Embodiment 3

[0019] Example 3: Add 10 g of powdered soybean lecithin to 40° C. and five times the volume of the ethanol extract containing 0.01% NaOH, stir and extract for one hour, centrifuge the extract, mix the residue with alkaline ethanol, Repeat the same operation twice, and the insoluble matter is vacuum-dried at 60° C. to obtain 4.46 g of powdered crude product of phosphatidylinositol, which contains 37.5 percent of phosphatidylinositol, 36.4 percent of phosphatidic acid, 1.7 percent of phosphatidylethanolamine, and phosphatidylinositol Alkali 0.6%. Weigh 1g of crude phosphatidylinositol and dissolve it in 60ml of n-hexane. After fully dissolving, put into a separatory funnel together with 100 ml of 55% aqueous ethanol dissolved with 0.325 g of anhydrous sodium acetate, shake at room temperature for 1 minute, and stand overnight. The aqueous ethanol layer was separated, 0.026 g of calcium chloride was added, stirred for 15 minutes, and left standing overnight at 4°C. The precipit...

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PUM

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Abstract

The invention relates to a high-purity PI preparing method. The solubility of PE is ov higher in basic alcohol than alcohol, using the basic alcohol to extract can effectively eliminate PC and PE from soybean phospholipids; dissolving the obtained PI crude product in a nonpolar solvent, adding in basic substance-containing hydrous polar solvent for liquid-liquid extraction, extracting PI into the hydrous polar solvent, and then adding in metallic salt for purifying, able to obtain high-purity PI.

Description

(1) Technical field [0001] The invention relates to a preparation method of high-purity phosphatidylinositol. (2) Background technology [0002] Phospholipids are widely distributed in organisms such as animals, plants, microorganisms, and algae, and are mainly used as constituents of cell membranes and play various important roles. Natural phospholipids are usually a mixture of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidic acid (PA) and sphingomyelin. Soybean lecithin, PC content 19-31%, PE content 18-20%, PI content 18-21%, PS content 1%, PA content 15-20%. [0003] Inositol lipid is a messenger system discovered in the 1980s that plays an important role in the transmembrane transmission of cell biological information, and is closely related to cell growth regulation. PI is one of the components of the inositol lipid system, accounting for about 5-10% of the total cell phospholipids. It widely ex...

Claims

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Application Information

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IPC IPC(8): C07F9/117
Inventor 刘代成安立国陶务端朱令仁
Owner SHANDONG NORMAL UNIV
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