Process for preparing macroporous cellulose sodium sulfate-polydimethyl diallyl ammonium chloride biolgoical micro capsule

A technology of polydimethyldiallylammonium chloride and cellulose sulfate, which is applied in the direction of microcapsule preparation and microsphere preparation, can solve the problems of pore-forming technology that have not yet been seen, and achieve easy application and promotion, biophase Good capacitive effect under mild conditions

Inactive Publication Date: 2005-03-02
ZHEJIANG UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, improving the structure of the capsule membrane and improving the transfer performance of the capsule membrane is a key factor for the microcapsule te

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of macroporous microcapsules

[0017] Prepare starch and sodium cellulose sulfate into a mixed solution of 0.7% to 1.5% starch and 0.5% to 5% sodium cellulose sulfate. The mixture is the polyanion solution of the system, and this solution is dropped into 1% to 8% of In the solution of polydimethyldiallylammonium chloride, the anion and cation interfaces react to form capsules, and the reaction time is 40 to 120 minutes. After the capsules are washed, they are transferred to 1 to 2% amylase solution and placed for 1 to 10 minutes. After 24 hours, wash again. Sodium cellulose sulfate NaCS is made by the Bioengineering Institute of Zhejiang University, and the specific preparation method can refer to literature [2, 3, 4].

Embodiment 2

[0018] Example 2: Soluble starch is used as porogen to prepare macroporous microcapsules

[0019] Prepare soluble starch and sodium cellulose sulfate into a mixed solution of 0.7% to 1.5% soluble starch and 3.5% to 5% sodium cellulose sulfate. The mixture is the polyanion solution of the system. % polydimethyldiallyl ammonium chloride solution, the anion-cation interface reacts to form capsules, and the reaction time is 40-60 minutes. After the capsules are washed, they are transferred to 1-2% amylase solution and placed After 18-24 hours, wash again.

Embodiment 3

[0020] Example 3: Soluble starch is used as porogen to prepare macroporous microcapsules

[0021] Prepare an aqueous solution of 1% soluble starch, add 4% sodium cellulose, stir for 3 hours, drop this solution into 8% polydimethyldiallylammonium chloride through a syringe, and react with cations and anions for 40 minutes to obtain capsules , after washing with deionized water, transfer to 1-2% amylase, no starch in the capsule can be detected after 24 hours (detection method: iodine solution and starch solution are blue), take out the capsule, wash with deionized water again, Store in 0.9% NaCl solution. The starch in the capsule is degraded due to the entry of amylase. The molecular weight of the amylase is 50,000. It is confirmed by the molecular weight cut-off experiment that the molecular weight cut-off of the capsule membrane is above 50,000.

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Abstract

The preparation process of macroporous sodium cellulose sulfate-polydimethyl diallyl ammonium chloride biological microcapsule includes: compounding starch and sodium cellulose sulfate into mixed solution with starch in 0.7-1.5 wt% and sodium cellulose sulfate in 0.5-5 wt% as the anion polymer solution for the system; dropping the mixed solution into polydimethyl diallyl ammonium chloride solution to form microcapsule through the interface reaction between anion and cation for 40-120 min; washing the microcapsule; transferring to amylase solution of 1-2 % concentration, setting for 1-24 hr and re-washing. The present invention has independently regulated membrane permeability, microcapsule membrane characteristic altered via changing the sort and amount of starch, good biocompatibility of the materials, simple preparation process, and mild condition.

Description

technical field [0001] The invention relates to a preparation method of macroporous sodium cellulose sulfate-polydimethyldiallylammonium chloride biological microcapsules. Background technique [0002] Cell microencapsulation technology is to encapsulate living cells in spherical microcapsules to provide cells with an ideal living state. At the same time, the nutrients in the medium and cell secretion products can freely pass through the semi-permeable membrane, thereby achieving catalysis and high density. The purpose of culture, immune isolation. At present, this technology has been used in high-yield continuous culture of microorganisms, plants, and animal cells and artificial cell transplantation. At present, the main cell microencapsulation systems are alginic acid, chitosan, polyacrylate, agar and so on. The cellulose sodium-polydimethyldiallyl ammonium chloride system invented by Dauzenberg et al. [1] has become a cell-based cell because of...

Claims

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Application Information

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IPC IPC(8): B01J13/02
Inventor 姚善泾张俊关怡新林东强
Owner ZHEJIANG UNIV
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