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Method for modifying plant morphology, biochemistry and physiology including expression plant fission agent oxidase

A cytokinin, plant technology, applied in the fields of morphology, biochemistry and physiology of modified plants including the expression of phytokinin oxidase, which can solve problems such as limitations

Inactive Publication Date: 2005-08-03
托马斯·施穆林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the main problem with methods relying on increased auxin synthesis is the problem of limitation, i.e., limiting the action of auxin to the root
It is not possible to overcome the problem of limitation by using tissue-specific promoters: auxins are transported in plants, so their action is not limited to the site of synthesis
Until now, it has not been possible to answer the opposite question: what are the consequences for plant growth and development if the concentration of endogenous cytokinins is reduced? Plants with reduced cytokinin content are expected to yield more accurate information about processes that are cytokinin-limited and thus potentially regulated

Method used

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  • Method for modifying plant morphology, biochemistry and physiology including expression plant fission agent oxidase
  • Method for modifying plant morphology, biochemistry and physiology including expression plant fission agent oxidase
  • Method for modifying plant morphology, biochemistry and physiology including expression plant fission agent oxidase

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Effect test

Embodiment approach

[0084] According to a first embodiment, the present invention relates to a method for stimulating root growth and / or increasing the formation of lateral roots and / or adventitious roots and / or changing root geotropism, which method includes the expression of plant cytokinin oxidase or includes reducing plants or plants The expression of another protein at the level of active cytokinin in the part.

[0085] In another embodiment, the present invention relates to increasing plant seed size and / or weight by increasing the activity or level of cytokinin oxidase in the plant or by reducing the expression of another protein that reduces the level of active cytokinin in the plant or plant part Methods. Preferably, the increased level or activity of cytokinin oxidase or the expression of another protein that reduces the level of active cytokinin in the plant or plant part is localized in different tissues or cell types of the seed, including the seed.

[0086] In another embodiment, the pr...

Embodiment 2

[0339] Example 2. Compilation of candidate cytokinin oxidases from Arabidopsis thaliana Identification of coding genes

[0340] Identification of Arabidopsis thaliana (Morris et al., Biochem Biophys Res Comm 255: 328-333, 1999; Houda-Herin et al. Plant J 17: 615-626; WO99 / 06571) having sequence similarity to the maize cytokinin oxidase gene (Morris et al., Biochem Biophys Res Comm 255:328-333, 1999; Arabidopsis thaliana) 6 different genes. Using the tblastn program, these genes were found by screening 6-frame translations of the nucleotide sequence of the public genome database using corn protein sequences. This sequence was named Arabidopsis thaliana cytokinin oxidase-like gene or AtCKX. They are randomly numbered as AtCKX1 to AtCKX6. The following list summarizes the information about these genes. The predicted ORF boundary and protein sequence are indicated to approximate the protein sequence differences between Arabidopsis and corn cytokinin oxidase, and between different Ar...

Embodiment 3

[0437] Example 3. Transgenic plants overexpressing AtCKX1 show increased cytokinin oxidation Enzyme activity and altered plant morphology

[0438] 1. Description of the cloning method

[0439] Use the following primers to amplify the AtCKX1 gene from Arabidopsis thaliana with the login name Columbia PCR (the non-homologous sequences used for cloning are in lowercase letters),

[0440] Sequence of 5'primer: cggtcgacATGGGATTGACCTCATCCTTACG (SEQ ID NO: 13)

[0441] Sequence of 3'primer: gcgtcgacTTATACAGTTCTAGGTTTCGGCAGTAT (SEQ ID NO: 14)

[0442] The 2235-bp PCR fragment amplified by these primers was inserted into the SalI site of pUC19. The insert was sequenced, and it was confirmed that the PCR amplified product did not contain any mutations. In the binary vector pBinHyg-Tx (Gatz et al., 1992) modified CaMV 35S promoter (with 3 tetracycline operator sequence) downstream SalI site subcloned the vector SalI / SalI fragment. Through Agrobacterium-mediated transformation, using standa...

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Abstract

Provided are methods for increasing seed size and / or weight, embryo size and / or weight, and cotyledon size and / or weight. The methods comprise expression of a plant cytokinin oxydase.

Description

Technical field [0001] Generally, the present invention relates to methods for modifying plant morphological, biochemical and physiological properties or characteristics, such as one or more developmental processes and / or environmental adaptation processes, including but not limited to the initiation, stimulation or Increase, and / or adventitious root formation, and / or lateral root formation, and / or root geotropism, and / or shoot growth, and / or apical dominance, and / or branching, and / or timing of senescence, and / or flowering Timing, and / or flower formation, and / or seed development, and / or modification of seed yield. Methods of increasing seed size and / or weight, increasing embryo size and / or weight, and increasing cotyledon size and / or weight are also provided. The method includes expressing in plants a cytokinin degradation control protein that is operably under the control of an adjustable promoter sequence, such as a cell-specific promoter, a tissue-specific promoter, or an organ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H5/00C07K16/40C12N1/15C12N1/19C12N1/21C12N5/10C12N9/02C12N9/06C12N15/09C12N15/29C12N15/53C12N15/82C12Q1/26G01N33/15G01N33/50G01N33/566
CPCC12N15/8295C12N9/0032
Inventor 托马斯·施穆林托马斯·维尔纳
Owner 托马斯·施穆林
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