Gibberella gene engineering bacterium and its preparation and application

A technology of genetically engineered bacteria and Gibberella, which is applied in the field of genetic engineering, can solve the problems of time-consuming and laborious results, unclear goals, and low potential, and achieve the effects of high efficiency, clear breeding goals, and strong capabilities
CN1654630AInactive Publication Date: 2005-08-17ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2005-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides one kind of genetically engineered bakanae fungus. The genetically engineered bakanae fungus is prepared through the process of: PCR amplification of rate-limiting enzyme gene cps / ks synthesized with gibberellin and powerful promoter Pgpd or PtrpC, cloning them to corresponding sites of the target plasmid capable of expressing in mycelial fungus to obtain super-expressing cps / ks gene vector with driving powerful promoter, transforming the super-expressing cps / ks gene vector into bakanae fungus to obtain the genetically engineered bakanae fungus. The present invention improves bakanae fungus by means of genetic engineering technology, and has determined fungus culturing target and high efficiency. The obtained engineered bakanae fungus has powerful gibberellin synthesizing ability, 40 % raised from the initial bakanae fungus strain.
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Description

(1) Technical field

[0001] The invention provides a gibberellin gene engineering bacterium capable of producing gibberellin and its preparation and application, belonging to the field of genetic engineering. (2) Background technology

[0002] Gibberellins (Gibberellins, GAs) is a plant growth regulator, which plays a very important role in plant growth and development. It can promote the internode elongation of plants, relieve the dormancy of seeds, tubers and buds, promote flowering of plants, induce parthenocarpy and inhibit senescence, etc. Gibberellins are widely used in fruits, vegetables, rice and other crops. Gibberellins can also be used in the beer production industry. In recent years, with the promotion and use of gibberellin, the market demand has been increasing.

[0003] Gibberellin is mainly produced by microbial fermentation, and the producing bacterium is the rice bakanae pathogen Gibberella fujikuroi, and gibberellin is the isoprene secondary metabolite o...

Claims

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