Monoclonal antibody for anti pig growth hormone, preparation process and application

A monoclonal antibody, growth hormone technology, applied in biochemical equipment and methods, botanical equipment and methods, anti-hormone immunoglobulin and other directions, can solve the problems of accumulation residues, human health hazards, etc. Market application prospects, good social and economic benefits
CN1687135AInactive Publication Date: 2005-10-26JILIN AGRICULTURAL UNIV +1
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN AGRICULTURAL UNIV
Publication Date
2005-10-26
Estimated Expiration
Not applicable Β· inactive patent
Patent Text Reader

Abstract

The present invention relates to a monoclonal antibody for resisting swine growth hormone, its preparation method and application. It includes heavy chain variable region and light chain variable region, every variable region has respective amino acid sequence. Said invention utilizes the screening process to obtain the hybridoma strain with stable secretion swine growth hormone monoclonal antibody, and can prepare monoclonal antibody with secretion specificity for resisting swine growth hormone, so that it can lay the foundation for further creating quick, flexible and specific swine growth hormone detection method and developing new-type growth promoter.
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Description

technical field

[0001] The present invention relates to the field of biotechnology. The invention utilizes technologies such as animal immunization, cell fusion and cloning to construct a hybridoma strain stably secreting a mouse anti-pig growth hormone monoclonal antibody, and prepare a specific monoclonal antibody secreting the porcine growth hormone. The present invention also relates to the preparation method of the monoclonal antibody and the application of the monoclonal antibody. Background technique

[0002] Growth hormone (GH) is an important hormone that promotes animal growth and regulates metabolism. It can promote glucose absorption, nucleic acid and protein synthesis, and fat breakdown. Porcine growth hormone is a single-chain polypeptide hormone composed of 190 amino acids secreted by the anterior lobe of the pig pituitary gland, with a molecular weight of 22kD. Hormone drugs were once widely used in modern animal production. However, its use has been banne...

Claims

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