Sensing method of protein chip and detection system therefor

Abstract

The invention relates to a sensing method of a protein array chip and its detecting system in the field of biology technique. The method comprises the following steps: letting the light reflected by the protein array chip to get through a one-dimensional magnifying lens array and inject into a time are phase modulator, changing the voltage to let the ejected s light and p light to get through the polarizing prism to generate intervention, changing the image into the electric signal by CCD after imaging lens and inputting it into the computer; when changing a phase of p light, it collects an image; using the collected five images as a circulation to obtain the time phase changing information aroused by the reaction of each unit of the array chip.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to high-precision, high-throughput, real-time sensing of protein-protein, protein-DNA, protein-effector, antigen-antibody, ligand-receptor, drug-target and other biological The method of molecular interaction and its protein chip detection system. Background technique [0002] Life science research has developed from gene sequence determination to elucidating the relationship between gene structure and function, that is, from genomics to proteomics. A gene is a kind of genetic material, composed of four bases, A, T, C, and G, which are strictly paired and have a relatively stable structure. The gene chip based on the principle of hybridization has become an important technical platform for genomics. Protein is the product of gene expression and the basic form of life activities. It has space-time characteristics, that is, different spatial structures have different functio...

AI Technical Summary

Problems solved by technology

However, this method has two disadvantages: one is that fluorescent or isotope labeling is required, which will affect the activity or binding site of the protein, thereby affecting the detection accuracy; the other is that it cannot quantify the detection of protein-protein interactions, which is precisely the Important content of omics research

Undoubtedly, both of these require high-precision, high-throughput, real-time sensing methods for detecting biomolecular interactions, and obviously none of the above four methods can meet the requirements

Images