Method for quickly detecting hepatitis B virus drug-resistant gene variation

A technology of hepatitis B virus and drug-resistant genes, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of mixed mutation detection, cumbersome experiments, and restrictions on wide application, so as to facilitate monitoring Efficacy, adjustment of treatment plan, effect of high amplification efficiency
CN1737164AInactive Publication Date: 2006-02-22SHANGHAI FUDAN YUEDA BIOTECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI FUDAN YUEDA BIOTECH
Publication Date
2006-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention, blonging to plant biotechnology field, relates a method of checking the virus of B hepatitis genovariation. The invention utilize the fluorescent light definite quantity PCR MGB Taqman probe technique, according to the YMDD nucleic acid sequence of B hepatitis virus, designing the genovariation of primer and probe checking HBV YMDD region. The invention adopts PCR reaction mixture A, B, Tap enzymes, HBV DNA extractant, and prepares diagnosis agent case after optimization and improvement with ddH20. The method can rapidly and exactly check the gene mutation situation of privileged site, quantitate the ratio of hybrid mutational virogene, quckly check the clinical hepatitis B drug therapia drug tolerance mutation. The method and agent case are not confined by medical equipment, so it is very easy to popularize.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a method for detecting hepatitis B virus related gene variation, in particular to a method for detecting hepatitis B virus lamivudine resistance related gene variation and a rapid diagnostic kit thereof. Background technique

[0002] In the reports on the incidence of statutory Class A and B infectious diseases over the years, hepatitis B has always ranked first, seriously threatening human health, and it is estimated that 2 billion people around the world are infected. According to the latest figures released by the Ministry of Health in August 2003, by the end of 2002, there were 130 million hepatitis B virus carriers nationwide, and more than 20 million chronic hepatitis B patients, accounting for 1.57% of the total population. 50% to 75% of them have active virus replication and liver inflammation changes, and some chronic hepatitis can progress to liver cirrhosis, liver fa...

Claims

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