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Gene recombination spider's thread protein high polymer organizational engineering porous stent material

A porous scaffold and gene recombination technology, applied in the field of biomedical materials, can solve the problems of collagen variability, insufficient mechanical strength, and strong antigenicity of collagen, and achieve the effects of simple processing technology, improved mechanical properties, and low cost

Inactive Publication Date: 2006-03-29
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to obtain ideal natural scaffold materials, and there are also some problems, such as strong antigenicity of collagen, insufficient mechanical strength, and easy denaturation of collagen during processing; although artificially synthesized substitutes are also biodegradable Features, such as US FDA-approved internal implant materials, have been made into absorbable sutures, splints, screws and dressings, etc., but these materials have poor biocompatibility, physical and chemical properties, degradation rate control and slow release needs to be improved

Method used

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  • Gene recombination spider's thread protein high polymer organizational engineering porous stent material
  • Gene recombination spider's thread protein high polymer organizational engineering porous stent material
  • Gene recombination spider's thread protein high polymer organizational engineering porous stent material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of scaffold preparation solution: Dissolve 2.25g gene recombinant spidroin protein powder in 7.5ml 98% formic acid to obtain 30% (w / v) protein solution, then add 0.45g polyvinyl alcohol, mix well, and obtain gene recombination Scaffold preparation solution of spidroin and polyvinyl alcohol.

[0027] 2. Add porogen: add 1.5g porogen sodium chloride and mix well.

[0028] 3. Mold pouring and heating: Pour the stent preparation solution added with porogen into a cylindrical mold (R=2cm), and put it in an oven at 70°C for 30 minutes.

[0029] 4. Denaturation and immersion in distilled water: soak the stent in ethanol for 15 hours for denaturation and distilled water for 10 hours.

[0030] 5. Freeze-drying: The denatured scaffold is frozen at -70°C for 2 hours to obtain a porous scaffold material.

Embodiment 2

[0032] 1. Preparation of scaffold preparation solution: Dissolve 2.5g of recombinant spidroin protein powder in 10ml98% formic acid to obtain a 25% (w / v) protein solution, add 1.25g of chitosan, and mix well to obtain recombinant spidroin protein containing gene and chitosan scaffold preparation solution.

[0033] 2. Add porogen: Add 2.0g porogen sodium chloride and mix well.

[0034] 3. Molding and heating: Pour the stent preparation solution added with the porogen into a rectangular mold (4cm×3cm×3cm), and put it in an oven at 60°C for 20 minutes.

[0035] 4. Denaturation and immersion in distilled water: soak the stent in ethanol for 10 hours for denaturation, and soak in distilled water for 8 hours.

[0036] 5. Freeze-drying: The denatured scaffold is frozen at -70°C for 4 hours to obtain a porous scaffold material.

Embodiment 3

[0038] 1. Preparation of scaffold preparation solution: get 2g gene recombinant spidroin protein powder and dissolve it in 6ml98% formic acid to obtain 33% (w / v) protein solution, then add 1.0g chitosan and 0.25g polyvinyl alcohol, mix well to obtain Scaffold preparation solution containing gene recombinant spidroin, chitosan and polyvinyl alcohol.

[0039] 2. Add porogen: Add 1.0g porogen sodium chloride and mix well.

[0040] 3. Mold pouring and heating: Pour the stent preparation solution added with the porogen into a rectangular mold (4cm×3cm×3cm), and put it in an oven at 65°C for 30 minutes.

[0041] 4. Denaturation and immersion in distilled water: soak the stent in ethanol for 13 hours and in distilled water for 12 hours.

[0042] 5. Freeze-drying: The denatured scaffold is frozen at -70°C for 3 hours to obtain a porous scaffold material.

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Abstract

A porous scaffold material for tissue engineering is prepared from the genetically recombinant arachnoid protein (55-95 mass %) and the biodegradable high polymer (5-45) through mixing. Its advantages are high biocompatibility, biodegradability and mechanical performance.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a tissue engineering scaffold material, especially a recombinant spidroin-high polymer porous scaffold material. Background technique [0002] The failure and damage of tissues and organs are the most important clinical medical problems, and the current treatment methods are mainly organ transplantation, surgical repair, and artificial replacement. Although these methods can play a certain role, they all have one or another. Insufficient, such as organ transplantation is a method of "healing wounds with wounds" at the expense of healthy tissues; existing artificial substitutes have biocompatibility problems. Until the 1980s, American scholars proposed tissue engineering regenerative medicine, which is to use the principles and methods of life science and engineering science to research and develop substitutes for replacing part or all of the functions of tissues or...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L27/26A61L27/56
Inventor 李敏陈登龙涂桂云黄曦周志华
Owner FUJIAN NORMAL UNIV