Chemical synthetic process of D-fluorescein and device therefor
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
- Publication Date
- 2006-04-05
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
[Affiliated technical field]
[0001] The invention belongs to the field of chemical synthesis, and relates to a chemical synthesis method and a reaction device of D-fluorescein. [Background technique]
[0002] D-luciferin is the substrate of ATP-dependent bioluminescent reaction, and the principle of bioluminescence is that luciferase in Mg 2++ , O 2 In the presence of ATP, D-luciferin is catalyzed to form oxidized luciferin, an enzymatic reaction process in which photons are emitted during the reaction, and the reaction formula is expressed as:
[0003] <chemistry num="001"> <chem file="200410051705_cml001.xml" / > < / chemistry>
[0004] Because the ATP in the reaction is the energy source of all organisms, under certain conditions, the intensity of the emitted light is proportional to the amount of ATP in the reaction system, so the bioluminescence method can be widely used in the detection of biological materials, such as the quantification of ultra-mic...