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Method for preparing water aqua for coutrolling cucumber downy mildew using tomato stem-leaf extract

A cucumber downy mildew, extract technology, applied in the field of pesticides, can solve the problem of relatively high cost, achieve good preventive effect, easy artificial cultivation, and simple extraction process

Inactive Publication Date: 2006-05-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After searching the prior art documents, it was found that Liu Rongchang mentioned in his patent (patent No. CN02113257.7) "a botanical fungicide for preventing and treating plant diseases and its preparation method" that tripterygium wilfordii, thunder pill, ginger, A green plant fungicide composed of garlic and wild cotton has a good control effect on cucumber downy mildew and powdery mildew. Its main active ingredient is tripterine B, and it also contains garlic antibiotic 402 and matrine relatively high cost
However, there is no research report on the use of tomato stem and leaf extracts in the control of cucumber downy mildew.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The percentage by weight of the water agent for controlling downy mildew of cucumber: 0.1% of tomato stem and leaf extract, and 99.9% of distilled water.

[0016] Weigh 40g of tomato stem and leaf powder (20 mesh particles), soak and extract twice with 25% ethanol at room temperature in 200ml, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 3.6g of dry matter; Suspend, extract with equal volumes of petroleum ether, ethyl acetate, and n-butanol successively for 5 times, concentrate the n-butanol phase extract to obtain 0.5 g of the extract, dissolve the extract with 100 ml of deionized water and filter, and prepare a concentration of 0.1% water agent; the indoor preventive test on cucumber downy mildew was carried out by using the leaf disk floating method, and the control effect on cucumber downy mildew reached 96.0%.

Embodiment 2

[0018] The weight percent of the water agent for controlling cucumber downy mildew: 1% of tomato stem and leaf extract, and 99% of distilled water.

[0019] Weigh 40g of tomato stem and leaf powder (20 mesh particles), soak and extract with 400ml of 75% ethanol at room temperature for 3 times, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 4.3g of dry matter; Suspend, extract with equal volume of petroleum ether, ethyl acetate, and n-butanol successively for 5 times, and concentrate the n-butanol phase extract to obtain 0.6 g of extract, dissolve the extract with 50 ml of distilled water, filter, and prepare a concentration of 1% Water agent; the indoor pot test is used to carry out the preventive test on cucumber downy mildew, and its control effect reaches 51.4%.

Embodiment 3

[0021] The weight percentage of water agent for controlling cucumber downy mildew: 3% of tomato stem and leaf extract, 97% of deionized water.

[0022] Weigh 200g of tomato stem and leaf powder (20 mesh particles), immerse and extract 4 times with 4000ml of absolute ethanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 16.6g of dry matter; use ultrasonic waves to suspend the dry matter with 500ml of water , an equal volume of petroleum ether, ethyl acetate, and n-butanol were extracted 5 times successively, and the n-butanol phase extract was concentrated to obtain 2.2 g of the extract. The extract was dissolved in 50 ml of deionized water, filtered, and the concentration was prepared to be 3%. The water agent; the field test was used to prevent cucumber downy mildew, and its control effect reached 48.88%. The control effect is significantly different from that of the blank control (spraying water).

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Abstract

The present invention relates to a method for preparing water solution agent for controlling downy mildew of cucumber by using tomato stem and leaf extract. Said water solution agent composition includes (by wt%) 0.1-3% of tomato stem and leaf extract and the rest is water. Said invented preparation method uses fresh tomato stem and leaf as raw material, and adopts the following several main steps: drying and pulverizing raw material, extracting by using ethyl alcohol as solvent, reduced pressure concentrating to obtain pasty extract, adding distilled water and utilizing ultrasonic wave generator to make ultrasonic treatment to make dried substance be suspended, then successively using petroleum ether, ethyl acetate and n-butyl alcohol to make extraction so as to obtain the invented product.

Description

technical field [0001] The invention relates to a preparation method in the technical field of pesticides, in particular to a method for preparing an aqueous agent for controlling cucumber downy mildew by using tomato stem and leaf extracts. Background technique [0002] Cucumber downy mildew is an important fungal disease that has plagued cucumber cultivation, especially protected cultivation in recent years. It is a worldwide disease that occurs in various parts of my country. Under suitable conditions, the epidemic speed is very fast, and it can be cured within two weeks. All leaves except the top young leaves die, and the yield reduction is as high as 30%-50%. At present, chemical pesticides are generally used to control such diseases, and it has become a consensus on the harm caused by chemical pesticides to the environment and human health, and the resistance of the controlled objects. The purpose of the invention of this patent is to control the occurrence of this dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/38A01P1/00
Inventor 代光辉赵杰金素心张志远
Owner SHANGHAI JIAO TONG UNIV
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