Recombinant adenovirus capable of expressing tomour specific apoptosis peptide, and its preparing method and use

A technology of recombinant adenovirus and recombinant virus, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of high production cost of synthetic peptides, increased treatment costs, and limited wide application

Inactive Publication Date: 2006-05-24
DOMINO MEDICINE INST BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0026] However, since various peptides are mainly prepared by synthetic methods at present, the yield is in milligrams, which is difficult to reach the level of industrial production; and the production cost of synthetic peptides is very high; there are a large number of peptidases in the circulating blood and cells of the human body. It is often rapidly degraded in the body, and the half-life in the body is shortened. Clinically, multiple daily injections of biological peptides are required to treat diseases, which increases the cost of treatment.
The above reasons limit the wide application of peptides as therapeutic drugs in clinical practice.

Method used

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  • Recombinant adenovirus capable of expressing tomour specific apoptosis peptide, and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Preparation of recombinant adenovirus capable of expressing tumor-specific apoptosis peptide

[0092] 1. Cell bank

[0093] The packaging cells are human embryonic kidney cells transduced with adenovirus packaging protein (purchased from ATCC, USA). , 20 vials of liquid nitrogen cryopreservation, as the original cell bank (primary seed cell bank), mark the parameters such as freezing time and algebra.

[0094] 1.1 Seed cell bank

[0095] It is stored in a special liquid nitrogen storage container, and a special person is responsible for it. Resuscitate one tube every two months, proliferate for another 2 generations, freeze 10 tubes, and mark the frozen storage time, generation number and other parameters. Every 10 generations, it has to go through FLC, morphological observation and identification of value-added ability. By analogy, the main cell bank is subcultured to 50 passages, terminated, and repurchased again. The identification method and monitoring...

Embodiment 2

[0131] Preparation and molding of embodiment 2 recombinant virus injection

[0132] Preparation and molding of recombinant virus injection

[0133] Dilute the purified recombinant virus into a 0.85% NaCl solution containing 2% mannitol to a final concentration of 2 × 10 8 pfu, filled to obtain the recombinant virus injection.

Embodiment 3

[0134] Preparation and molding of embodiment 3 recombinant virus injection

[0135] Preparation and molding of recombinant virus sterile powder

[0136] Dilute the purified recombinant virus into a 0.85% NaCl solution containing 2% mannitol to a final concentration of 2 × 10 8 pfu, filled and freeze-dried to obtain sterile powder of the recombinant virus.

[0137] Dosage Forms and Specifications for Clinical Application

[0138] 1. Injection (10 -10 ~10 10 ml / specification)

[0139] 2. Sterile powder for injection (10 -10 ~10 10 mg / specification)

[0140] clinical route of administration

[0141] 1. Subcutaneous injection

[0142] 2. Intradermal injection

[0143] 3. Intramuscular injection

[0144] 4. Intravenous injection

[0145] 5. Intravenous infusion

[0146] 6. In vivo Tumor Injection

[0147] 7. Vascular catheterization, etc.

[0148] Clinical application scope:

[0149] 1. Glioma

[0150] 2. Liver cancer

[0151] 3. Lung cancer

[0152] 4. Breast ca...

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Abstract

The invention discloses rebuild adenovirus that could express tumor distinguished apoptosis peptide and the method to manufacture. It rebuilds the adenovirus carrier and inducing the material that make the tumor apoptosis to achieve the aim of cure tumor.

Description

technical field [0001] The invention relates to a recombinant adenovirus capable of expressing tumor-specific apoptosis peptide and a preparation method thereof. Background technique [0002] Tumor is a disease that seriously threatens human health, and its harm to human health is self-evident. Scientists and governments all over the world have invested huge manpower and material resources in decades-long research. Some valuable research results have been obtained, but it can be said that so far, no breakthroughs have been made. Surgical resection, radiotherapy and chemotherapy are still the "three magic weapons" for tumor treatment. Immunotherapy was once considered the fourth magic weapon, but immunological interventions including immune regulation, tumor-specific monoclonal antibodies, and LAK cells have not achieved the expected results. [0003] In recent years, with the gradual deepening of people's understanding of tumor biology and tumorigenesis, some biological ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K39/235A61P35/00C12N15/12C12N15/861
Inventor 杨广孝李求是
Owner DOMINO MEDICINE INST BEIJING
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