Renaturation of reconstituted human bone protein-1 and making method of its preparation

An osteogenic protein and preparation technology, applied in the field of biotechnology and pharmacy, can solve the problems of high cost, limited use, low yield and the like, and achieve the effect of simple and easy renaturation process
CN1786017AInactive Publication Date: 2006-06-14SHANDONG MEDICAL BIO TECH RES CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG MEDICAL BIO TECH RES CENT
Publication Date
2006-06-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a recombinant human osteoplastic protein-1 renaturation and its preparation preparation method. It utilizes chromatography to obtain rh OP-1 monomer protein whose purity is above 95%. Said invention also provides the concrete steps of said preparation method, including: in 2M-6M urea or 1M-4M guanidine hydrochloride buffer solution making dialysis and renaturation, pH value of renaturation buffer solution is 8.0-10, renaturation temperature is room temperature or 4deg.C and renaturation time is 24-96 hr, then further making chromatographic purification, dialysis, sterilization, packaging and freeze-drying so as to obtain the renaturation preparation of recombinant human osteoplastic protein-1. Besides, said invention also provides the application method of said renaturation preparation of recombinant human osteoplastic protein-1.
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Description

Technical field:

[0001] The invention relates to a renaturation of osteogenic protein-1 and a preparation method thereof, in particular to a renaturation of recombinant human osteogenic protein-1 (rhOP-1) and a preparation method thereof. It uses genetic engineering means to express and mass-produce recombinant human osteogenic protein-1 mature peptide in Escherichia coli to refold recombinant human osteogenic protein-1 and prepare its preparations. After sterilization, the preparation prepared by the invention can be clinically used for repairing and orthopedic bone defects, and for maintaining and recovering alveolar bone in oral cavity tooth extraction wounds and the like. It belongs to the field of biotechnology and pharmaceuticals. Background technique:

[0002] With the rapid development of genetic engineering technology, more and more people provide some protein polypeptide products for clinical and industrial production through heterologous expression of proteins. ...

Claims

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