12 amino acid analog epi-position of human b cell specificity membrane molecule CD20 and polypeptide epi-position vaccine configurated by said analog epi-position

A technology of mimetic epitope and specificity, applied in the field of medicine and biology, can solve the problem of low homology, and achieve the effect of reducing the side effects of monoclonal antibodies and reducing the burden on patients
CN1786021AInactive Publication Date: 2006-06-14FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
FOURTH MILITARY MEDICAL UNIVERSITY
Publication Date
2006-06-14
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The present invention discloses a simulation epitope of 12 amino acids of human cell B specificity expression membrane molecule CD 20 and its polypeptide epitope vaccine constructed by using said simulation spitope. Said invention also provides a method for screening simulation epitope of CD 20 molecule by using Rituximab as ligand, and provides its amino acid sequence, Gln-Asp-Lys-Leu-Th-Gln-Try-Pro-Lys-Try-Leu-Glu. Said invention also provides a method for chemically-synthesizing simulation epitope of 12 amino acids and making it be chemically-coupled with keyhole limpet hemo cyanin (KLH) to obtain the successfully-constructed vaccine. Besides, said invention also provides the concrete application of said vaccine.
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Description

technical field

[0001] The invention belongs to the field of medical biotechnology, and specifically relates to screening of phage randomly presented peptide libraries, affinity detection and sequencing of screened phages, synthesis and coupling of polypeptides, animal immunization experiments, in vitro activity detection of induced antiserum and the like. It further relates to a 12-amino acid mimic epitope of human B cell-specific membrane molecule CD20 and a polypeptide epitope vaccine constructed by using the mimic epitope and capable of inducing autoantibodies against CD20 molecules in vivo. Background technique

[0002] 1. Research on CD20 molecule and its monoclonal antibody

[0003] 1.1 CD20 molecule

[0004] The CD20 molecule is a marker molecule expressed on the surface of more than 95% of normal or deteriorated B cells, and is an ideal target antigen for the treatment of B cell lymphoma. The gene encoding CD20 is located in the t(11...

Claims

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