Molecular design and prepn process of recombinant polyepitope chimeric peptide CP22 antigen of human chorionic gonadotropin
A chorionic gonadotropin, CP22 technology, applied in the field of genetic engineering, can solve the problems of poor performance and undeveloped synthetic peptide vaccines
Inactive Publication Date: 2006-08-02
SHANGHAI INST OF PLANNED PARENTHOOD RES +1
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But needless to say, the actual human clinical application also needs to overcome the problem of coupling with DT, the need for special adjuvants (to reduce the cost of the vaccine and facilitate the production of preparations), the specificity of the vaccine but the poor neutralization performance of the produced antibody against hCG, and how to improve and improving the immune response rate and therapeutic efficacy of tumor patients with different genetic backgrounds after vaccination
[0003] On the other hand, the development of chemically synthesized peptide vaccines has always been expected to contribute to the prevention and treatment of human diseases. However, in the past forty years, a synthetic peptide vaccine that can be practically used has not been developed, although it has many such as Obvious advantages such as constant and relatively cheap rare antigens can be obtained
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[0046] Materials and methods
[0047] 1. Restriction enzymes EcoR I, Sal I, Nde I, and BamH I are products of Boehringer Mannheim in the United States, T4 DNA ligase is a product of British Biolabs, T4 polynucleotide kinase is a product of New England Biolabs in the United States, and Taq I DNA polymerase is a product of Products of the State Key Laboratory of Genetic Engineering of Fudan University, IPTG is a product of Promega in the United States, protein low molecular weight standards and alkaline phosphatase-linked goat anti-mouse secondary antibody (IgG / HRP) are products of Huamei Bioengineering Company, 5-bromo-4-chloro -3-Indole-β-D-galactoside (X-gal) and isopropylthio-β-D-galactoside (IPTG) are products of Sigma Company in the United States, which recognize hCGβ 133-139 The sequenced monoclonal antibody OT3A was a kind gift from Organon Technika, The Netherlands.
[0048] 2. Cloning and sequencing The vector pBluescript KS (PBS) was purchased from Stratagene, and th...
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The present invention provides recombinant polyepitope chimeric peptide CP22 of human chorionic gonadotropin, which consists of basically epitope peptide HBsAg19-33, epitope peptide HBcAg85-140 and epitope peptide TT580-599, as well as beta-5 epitope peptide, beta-8 epitope peptid and beta-9 epitope peptid of two human chorionic gonadotropins. The present invention also provides the nucleic acid sequence coding the chimeric peptide CP22, expression vector and host cell containing the sequence, and the preparation process of the chimeric peptide CP22. The chimeric peptide CP22 may be applied in preparing vaccine composition for treating tumor and / or controlling conception.
Description
technical field [0001] The invention relates to the technical field of genetic engineering, more specifically, the invention relates to human chorionic gonadotropin recombinant multi-epitope chimeric peptide CP22 antigen and a preparation method thereof. Background technique [0002] Human chorionic gonadotropin (hCG), also known as pregnancy hormone, is a glycoprotein hormone that maintains early pregnancy in humans. It consists of two subunits, α and β. In addition, it has also been identified as a carcinoembryonic antigen, which is expressed in many malignant tumors, for example, hCG can be detected in cancers of the pancreas, lung, colorectum, cervix, ovary, liver, stomach, etc. The existence of its subunits or fragments (Triozzi P and StevensVC. Human chorionic gonadotropin as a target for cancer vaccines. Oncol Rep 6: 7-17, 1999). Therefore, hCG has been considered as an important target antigen for the development of contraceptive and / or tumor therapeutic vaccines. ...
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IPC IPC(8): C07K14/59C12N15/12C12N15/63A61K39/00A61K38/24A61P35/00A61P15/18
Inventor 徐万祥谢毅廖矛川季朝能何亚萍顾少华洪爱真应康王键孙志达
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES