Water gel fast forming process based on bionic process

A forming process, hydrogel technology, applied in biomanufacturing and bionic manufacturing, tissue engineering material manufacturing and rapid prototyping, manufacturing science and other interdisciplinary fields, can solve the problem of difficult operation of hydrogel materials, achieve structural stability, Excellent biocompatibility and high porosity

Inactive Publication Date: 2006-08-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to solve the problem that hydrogel materials are difficult to operate, and provide a new hydrogel rapid prototyping process to realize the formation of three-dimensional hyd

Method used

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  • Water gel fast forming process based on bionic process

Examples

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Effect test

Embodiment 1

[0027] Example 1: Fibrin-gelatin three-dimensional structure produced by this process. The mixture of fibrinogen and gelatin is prepared into component I aqueous solution, the concentration is 0.050g / ml, wherein the mass ratio of fibrinogen and gelatin is 2: 3, then make certain calcium chloride, thrombin solution as component II, the concentration of thrombin is 5000U / ml, the concentration of calcium chloride is 0.05g / ml, and it is sterilized for use; the material is pre-cooled at low temperature first, and in an environment of 4°C, according to the pre-designed structure and defined planning path, By means of extrusion, component I is sprayed into the aseptic forming chamber of component II filled with atomization; after spraying to the designated position, component 1 undergoes a sol-gel transition to form a hydrogel; layer by layer accumulation is obtained Three-dimensional structure of fibrinogen gelatin.

Embodiment 2

[0028] Example 2: The fibrin-gelatin three-dimensional structure produced by this process. The mixture of fibrinogen and gelatin is prepared as component I aqueous solution, the concentration is 0.200g / ml, wherein the mass ratio of fibrinogen and gelatin is 1:3, and then a certain amount of thrombin and coagulation factor solution is used as component II , the concentration of thrombin is 2U / ml, the concentration of coagulation factor is 32U / ml, and it is sterilized for later use; the material is pre-cooled at low temperature, and in an environment of 4°C, according to the pre-designed structure and defined path, it is extruded The method, the component I is sprayed into the liquid component II environment; after spraying to the designated position, the component I undergoes a sol-gel transition to form a hydrogel; the fibrinogen-gelatin three-dimensional structure is obtained layer by layer.

Embodiment 3

[0029] Example 3: The three-dimensional structure of fibrin-agarose produced by this process. The mixture of fibrinogen and agarose is dissolved in calcium chloride solution to prepare component I aqueous solution, the concentration is 0.050g / ml, and it is sterilized for later use, wherein the mass ratio of fibrinogen and agarose is 3: 2, and then Thrombin and coagulation factors are embedded and fixed on the inner surface of the nozzle cavity; at 37°C, according to the pre-designed structure and defined path, component I is immobilized when it is sprayed through the nozzle by extrusion. The sol-gel transformation occurs to form a hydrogel through the action of the enzyme, and then the three-dimensional structure of fibrinogen-agarose is obtained through layer-by-layer accumulation.

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Abstract

The fast bionic water gel forming process simulates human blood coagulation process to produce fibrin gel. Fibrinogen and medical water soluble polymer material, as well as thrombin, Ca ion and blood coagulation factor are prepared into two multiple-component water solutions; and the two water solutions are made to mix and accumulate in certain spatial position by means of digital micro dropping and jetting technology while producing enzyme reaction to form water gel with fibrin as matrix, so as to make 3D water gel structure with complicated spatial shape and porous structure. The coagulation process has complicated and effective regulating and controlling mechanism, and the water gel structure has excellent mechanical and biological performance, stable structure and effectively regulated degradation speed. The present invention is suitable for forming tissue engineering sample, medicine slow releasing carrier, etc.

Description

technical field [0001] The invention relates to a hydrogel rapid prototyping process based on a bionic process, which belongs to the interdisciplinary technical fields of biomanufacturing and bionic manufacturing, manufacturing science and other disciplines. The invention belongs to the technical field of tissue engineering material manufacturing and rapid prototyping. Background technique [0002] Rapid prototyping technology is a comprehensive technology based on multiple technologies such as CAD, numerical control, laser and new materials. It is a general term for rapid manufacturing of complex-shaped three-dimensional physical entities directly driven by CAD models. Due to its characteristics, rapid prototyping has been widely used in many fields, and it has played a very important role in the medical field, especially in the scaffold construction of tissue engineering. In the study of tissue engineering, a very important aspect is the construction of tissue engineering...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/40
Inventor 熊卓颜永年李生杰王小红吴任东林峰张人佶卢清萍刘海霞
Owner TSINGHUA UNIV
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